Physicochemical Screening and Shelf Life Evaluation of Prepared using and .

Introduction: (KG) is an Ayurvedic formulation commonly recommended in skin disorders, especially (Acne vulgaris). (stigma of Linn.) is a prime ingredient in the formulation. However, due to high cost and increased adulteration in Kesara, '' ( Linn.) is suggested by Ayurvedic experts as a substitute. is relatively lower in cost and possesses similar therapeutic attributes to that of . Recent studies have established standard manufacturing procedures and clinical efficacies of (with ) (KKG) and (with ) (NKG) in . In spite of wide utility of KG in Ayurvedic practice, no published work on its shelf life is available so far.

Aims And Objectives: To establish the physicochemical profile and evaluate the shelf life of KKG and NKG.

Materials And Methods: Total three batches of KKG and NKG each were prepared in the laboratory by adopting Ayurvedic classical guidelines and the findings were systematically recorded. Comparative differences in organoleptic characters (colour, odour, taste, touch) and values of different physicochemical parameters (Ash value, pH value, Total solid content, Specific gravity, Refractive index, Loss on drying, Acid value, Iodine value, Saponification value, Peroxide value and Rancidity tests) of raw materials [herbal ingredients, , (MG)] as well as finished products (KKG and NKG) were recorded. Long term (real time) shelf life testing was carried out at the intervals i.e., at 0, 3, 6, 9 and 16 months. High performance thin layer chromatography (HPTLC) analysis of samples was also carried out.

Results And Conclusion: The average percentage loss in final product was 10.18% in KKG and 7.58% in NKG. The shelf life of MG, KKG and NKG is 16 months. After 16 months, showed negative rancidity test, while MG, KKG and NKG showed positive rancidity, indicative of onset of deterioration after 16 months. Present findings validate the Ayurvedic principles stipulating medicated to have a shelf life of 16 months. Chromatographic study showed few differences between KKG and NKG samples. Data obtained by present study may be considered as standard for future researches.