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is a biotrophic smut fungus infecting sp. It is closely related to the barley-infecting smut and related to the well-studied, gall-inducing model pathogen Upon flowering, the spikelets of infected plants are filled with black fungal spores. While it is possible to directly use this spore material to infect seeds, in many cases it is more useful to isolate individual strains of for a genetically homogenous population This protocol describes how to collect and germinate the spores of on plate in order to obtain strains derived from a single cell.
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http://dx.doi.org/10.21769/BioProtoc.2392 | DOI Listing |
Int J Syst Evol Microbiol
September 2025
School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland.
Two yeast strains, PYCC 10015 and PYCC 10016, were isolated from soil from an Irish forest. Sequence analysis of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rRNA gene repeat, and the D1/D2 domain of the LSU rRNA gene, showed that they belong to the and genera of the order , but they did not exactly match any known species.
View Article and Find Full Text PDFProbiotics Antimicrob Proteins
September 2025
Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India.
Ethnic fermented foods represent a significant repository for discovering novel probiotic entities. These fermented foods, entrenched in indigenous practices, have conserved a distinct microbiota through generations. Exploration of these fermented foods could yield microbial consortia capable of transforming human health.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
September 2025
School of Plant Sciences, The University of Arizona, 1140 E South Campus Drive, Forbes 303, Tucson, AZ, 85721, USA.
Fungal endophytes and epiphytes associated with plant leaves can play important ecological roles through the production of specialized metabolites encoded by biosynthetic gene clusters (BGCs). However, their functional capacity, especially in crops like lettuce (Lactuca sativa L.), remains poorly understood.
View Article and Find Full Text PDFArch Microbiol
September 2025
División de Ciencias Naturales y Exactas, Departamento de Biología, Universidad de Guanajuato, Zip Code 36050, Guanajuato, Mexico.
Plasmids are fundamental to molecular biology and biotechnology, playing a crucial role in bacterial evolution. Some plasmids are linked to complex cellular dynamics, including pathogenicity islands, antibiotic resistance, and gene mobilization. This study reports the isolation and sequencing of two cryptic plasmids with different electrophoretic mobilities from the Escherichia coli clinical isolate O55.
View Article and Find Full Text PDFCurr Microbiol
September 2025
Microbiology Laboratory, Department of Life Science, Kyonggi University, Suwon, Gyeonggi-Do, Republic of Korea.
A yellow-pigmented, non-motile, rod-shaped, and Gram-stain-negative bacterium was isolated from the soil of Yeongheung Island, Korea. The novel isolate, strain N803, was strictly aerobic, grew optimally at 30-35 °C, at pH 6.5, and in the presence of 0-2% NaCl.
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