Isolation of Strains from Infected Spikelets through Spore Recovery and Germination.

Bio Protoc

Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria.

Published: July 2017


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Article Abstract

is a biotrophic smut fungus infecting sp. It is closely related to the barley-infecting smut and related to the well-studied, gall-inducing model pathogen Upon flowering, the spikelets of infected plants are filled with black fungal spores. While it is possible to directly use this spore material to infect seeds, in many cases it is more useful to isolate individual strains of for a genetically homogenous population This protocol describes how to collect and germinate the spores of on plate in order to obtain strains derived from a single cell.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540166PMC
http://dx.doi.org/10.21769/BioProtoc.2392DOI Listing

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