Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Cancer stem cells or tumor-initiating cells (TICs) are a small subpopulation of cells that have the capacity to self-renew, differentiate and initiate tumors. These cells may function in tumor initiation, aggression and recurrence. Whether spheres derived from canine rhabdomyosarcoma cells have stem cell-like properties is unclear. We induced sphere formation in the canine rhabdomyosarcoma cell lines, CMS-C and CMS-J, and characterized the spheres in vitro and in vivo. Sphere-forming cells were more resistant to vincristine, mitoxantrone and doxorubicin than adherent cells. Xenograft transplantation demonstrated that 1 × 10 sphere-forming cells derived from CMS-C were sufficient for tumor formation. The sphere assay showed that the sphere-forming cells were present in these tumors. These results suggest that the spheres derived from canine rhabdomyosarcoma cells may possess characteristics of TICs. This study provides the foundation for elucidating the contribution of TICs to rhabdomyosarcoma tumorigenesis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559357 | PMC |
| http://dx.doi.org/10.1292/jvms.16-0412 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identifying functional roles and pathways of shared mutations in canine solid tumors by whole-genome sequencing.
PLoS One
May 2025
College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea.
Identifying genetic mutations contributing to solid tumors by altering the biological pathways related to tumor formation and development is essential for the development of targeted therapies. This study aimed to identify commonly mutated genes and altered pathways in canine solid tumors. Four dogs with different types of naturally occurring neoplasias (urothelial carcinoma, adenocarcinoma, rhabdomyosarcoma, and chondrosarcoma) were randomly selected and classified into carcinoma and sarcoma groups based on histopathological findings.
View Article and Find Full Text PDFCutaneous melanoma with heterologous rhabdomyosarcomatous component in a dog.
J Comp Pathol
May 2025
Universidad Autónoma de Nuevo León, 66054, Cd Gral Escobedo, Nuevo León, Mexico. Electronic address:
Melanocyte-derived tumours are frequent in humans and animals and the clinicopathological progression of malignant presentations in dogs is comparable with humans. However, melanocytic tumours are a heterogeneous group of neoplasms with certain morphopathological patterns that may appear completely distant from the expected origin. Biphasic neoplasms with heterologous components representing transdifferentiation are uncommon and represent a challenge in diagnosis.
View Article and Find Full Text PDFCD117 (KIT) in canine soft tissue sarcoma: an immunohistochemical and c-kit gene mutation assessment.
Front Vet Sci
April 2025
Department of Veterinary Medicine and Animal Sciences-DIVAS, University of Milano, Lodi, Italy.
Introduction: Canine soft tissue sarcomas (STSs) are locally aggressive mesenchymal tumors with variable recurrence rates, and often, their therapy is limited to surgical excision. CD117 (KIT) is a tyrosine kinase receptor involved in cell growth and cancer development. c-kit proto-oncogene mutations have been reported to be associated with prognosis and therapy response in human and canine cancers.
View Article and Find Full Text PDFA Case Report on Alveolar Rhabdomyosarcoma in an Indigenous Dog.
Vet Med Sci
November 2024
Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh.
A 12 years-old, male indigenous dog was presented to Shahedul Alam Qaderi Teaching Veterinary Hospital (SAQTVH) in Chattogram, Bangladesh with a large tumour on its right front leg. The histopathological findings of the tumour were highly suggestive of an alveolar rhabdomyosarcoma (ARMS). The tumour was surgically removed from the dog.
View Article and Find Full Text PDFA case series of urinary bladder rhabdomyosarcoma in seven dogs.
Open Vet J
November 2023
Endovet Professional Association, Roma, Italy.
Background: Juvenile urinary bladder rhabdomyosarcoma (ubRMS) is a known entity; however, literature regarding its clinical behavior and endoscopic features is scarce. The aim of this study was to describe clinical and endoscopic features, and outcomes of ubRMS in dogs.
Case Description: Dogs undergoing transurethral endoscopy and with a histological diagnosis of ubRMS were retrospectively collected.