Large-scale Phenotypic Profiling of Gene Deletion Mutants in .

Bio Protoc

Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.

Published: July 2015


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Here, we describe a method enabling the phenotypic profiling of genome-scale deletion collections of fungal mutants to detect phenotypes for various stress conditions. These stress conditions include among many others antifungal drug susceptibility, temperature-induced and osmotic as well as heavy metal or oxidative stress. The protocol was extensively used to phenotype a collection of gene deletion mutants in the human fungal pathogen () (Schwarzmüller , 2014).

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5074359PMC
http://dx.doi.org/10.21769/BioProtoc.1530DOI Listing

Publication Analysis

Top Keywords

phenotypic profiling
8
gene deletion
8
deletion mutants
8
stress conditions
8
large-scale phenotypic
4
profiling gene
4
mutants describe
4
describe method
4
method enabling
4
enabling phenotypic
4

Similar Publications

Epidemiology, resistance profiles, and risk factors of multidrug- and carbapenem-resistant Serratia marcescens infections: a retrospective study of 242 cases.

BMC Infect Dis

September 2025

Department of Laboratory Medicine, Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, China.

Background: Serratia marcescens is an opportunistic pathogen increasingly associated with healthcare-associated infections and rising antimicrobial resistance. The emergence of multidrug-resistant (MDR) and carbapenem-resistant S. marcescens (CRSM) presents significant therapeutic challenges.

View Article and Find Full Text PDF

Mechanisms and treatment of cancer therapy-induced peripheral and central neurotoxicity.

Nat Rev Cancer

September 2025

Department of Neurology, Division of Neuro-Oncology, Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA, USA.

Neurotoxicity is a common and potentially severe adverse effect from conventional and novel cancer therapy. The mechanisms that underlie clinical symptoms of central and peripheral nervous system injury remain incompletely understood. For conventional cytotoxic chemotherapy or radiotherapy, direct toxicities to brain structures and neurovascular damage may result in myelin degradation and impaired neurogenesis, which eventually translates into delayed neurodegeneration accompanied by cognitive symptoms.

View Article and Find Full Text PDF

Frailty, often linked to sarcopenia, involves reduced muscle strength and mass. While sarcopenia has multiple causes, impaired muscle protein synthesis may contribute. Leucine and resistance training (RT) are anabolic stimuli, but the long-term effects of leucine combined with RT in pre/frail older women remain unclear.

View Article and Find Full Text PDF

CD4 T follicular helper (T) cells support tailored B cell responses against multiple classes of pathogens. To reveal how diverse T phenotypes are established, we profiled mouse T cells in response to viral, helminth and bacterial infection. We identified a core T signature that is distinct from CD4 T follicular regulatory and effector cells and identified pathogen-specific transcriptional modules that shape T function.

View Article and Find Full Text PDF

Suaeda salsa(S.salsa) is a promising halophytic species for vegetation restoration in highly saline-alkali soils. Carboxylated single-walled carbon nanotubes (COOH-SWCNTs) have emerged as potential agents for modulating plant responses to abiotic stress.

View Article and Find Full Text PDF