Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The intraocular route of drug administration enables the delivery of high concentrations of therapeutic drugs, while minimizing their systemic absorption. Several drugs are administered into the anterior chamber or vitreous, and the intraocular injection has been effective in curing various intraocular diseases. Rabbit eyes have been widely used for ophthalmic research, as the animal is easy to handle and economical compared to other mammals, and the size of a rabbit eye is similar to that of a human eye. Using a 30 G needle, drugs can be injected into the intracameral and intravitreal spaces of rabbit eyes. The eyeballs are then frozen until analysis, and can be divided into the aqueous humor, vitreous, and retina/choroid. The vitreous and retina/choroid samples can be homogenized and solubilized before analysis. Then, immunoassays can be performed to measure the concentrations of intraocular drugs in each compartment. Appropriate pharmacokinetic models can be used to calculate several parameters, such as the half-life and maximum concentration of the drug. Rabbit eyes can be a good model for pharmacokinetic studies of intraocular drugs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091672 | PMC |
| http://dx.doi.org/10.3791/53878 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of Growth and Development on Aqueous Humor Dynamics in Male Dutch Belted Rabbits.
Invest Ophthalmol Vis Sci
September 2025
Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, Nebraska, United States.
Purpose: This study reports the changes in aqueous humor dynamics (AHD) and biometric parameters associated with growth and maturation in male Dutch-belted rabbits.
Methods: In this prospective observational study, 20 male Dutch-belted rabbits were studied at 4-weekly intervals starting at 10 weeks through 38 weeks of age. Data collected included intraocular pressure (IOP), aqueous flow, outflow facility by tonography and fluorophotometry (acetazolamide method), episcleral venomanometry, central corneal thickness, corneal diameter, anterior chamber depth, and testicular volume.
Characterization of hydrolase activity in ocular tissues of humans and preclinical species.
Drug Metab Dispos
August 2025
Department of Drug Metabolism and Pharmacokinetics, Genentech, Inc, South San Francisco, California. Electronic address:
Hydrolases in the eye play an important role in the metabolism of ophthalmic drugs, especially those administered locally to the eyes. With the growing interest in peptide-based therapeutics for treating eye disease, it has become increasingly important to characterize the enzymatic activities of ocular tissues against both small molecules and peptides to better understand their ocular metabolism. In this study, we characterized the activities of hydrolases, including carboxylesterase 1 and 2, arylacetamide deacetylase, paraoxonases, cytidine deaminase, fatty-acid amide hydrolase, and peptidases by incubating probe substrates in whole eye homogenates and vitreous humors from human donors and 3 preclinical species, including New Zealand White rabbits, Gottingen minipigs, and Cynomolgus monkeys.
View Article and Find Full Text PDFSuprachoroidal Injection of Topotecan for Retinoblastoma: A Preclinical Study.
Ophthalmol Sci
July 2025
Vanderbilt University Medical Center, Nashville, Tennessee.
Purpose: To assess whether levels of topotecan that are expected to be therapeutic against retinoblastoma tumors can be achieved within the retina and choroid by suprachoroidal injection (SCI) and to assess toxicity and safety in vivo.
Design: Pharmacokinetics and dose escalation toxicity study.
Subjects: New Zealand white rabbits.
Effect of Periplaneta americana Extract on Corneal Fibrosis and Epithelial Healing After Rabbit Lamellar Keratectomy.
Transl Vis Sci Technol
September 2025
Ineye Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Purpose: To evaluate Periplaneta americana extract (PAE) effects on corneal epithelial healing and fibrosis after superficial lamellar keratectomy (SLK) in rabbits.
Methods: SLK was performed on the right eyes of 48 New Zealand White rabbits, randomized into three treatment groups (n = 16/group): normal saline (NS), Tobradex eye drops (TE), and PAE group. Corneal opacity and epithelial defect area were quantified using slit-lamp imaging at postoperative days 3, 7, 14, and 28 (D3, D7, D14, and D28) and scored via the grading system.
An in vitro pre-screening model to evaluate the corneal anti-inflammatory effect of human blood-derived products and amniotic membrane extracts incorporated into gelatine-based hydrogels.
Exp Eye Res
September 2025
Department of Cell Biology and Histology, School of Medicine and Nursing, University of the Basque Country UPV/EHU, 48940, Leioa, Spain; BEGIKER Ophthalmology Research Group, Biobizkaia Health Research Institute, 48903, Barakaldo, Spain. Electronic address:
The objective of this study was to develop a reliable, cost-effective, and rapid in vitro model employing real-time PCR to assess inflammatory responses in hydrogel-based systems, and to comparatively evaluate the anti-inflammatory efficacy of human serum (HS), serum derived from plasma rich in growth factors (sPRGF), and human amniotic membrane extracts (HAMe) incorporated into gelatin-based hydrogels. An in vitro model of corneal inflammation was established by quantifying IL-1β expression via qPCR in TNFα-stimulated SV-40 immortalised human corneal epithelial (HCE) cells. Hydrogels functionalised with HS, sPRGF, or HAMe sourced from proximal, medial, distal, or pooled amniotic regions were evaluated for their anti-inflammatory potential.
View Article and Find Full Text PDF