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[This corrects the article DOI: 10.1371/journal.pone.0107349.].
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J Vis Exp
September 2025
Department of Biochemistry & Molecular Biology, Dalhousie University; Department of Chemistry, Dalhousie University; School of Biomedical Engineering, Dalhousie University;
This corrects the article 10.3791/68714.
View Article and Find Full Text PDFNAR Genom Bioinform
September 2025
DNA Repair and Recombination Laboratory, St Vincent's Institute of Medical Research, Fitzroy VIC 3065, Australia.
Meiotic crossovers promote correct chromosome segregation and the shuffling of genetic diversity. However, the measurement of crossovers remains challenging, impeding our ability to decipher the molecular mechanisms that are necessary for their formation and regulation. Here we demonstrate a novel repurposing of the single-nucleus Assay for Transposase Accessible Chromatin with sequencing (snATAC-seq) as a simple and high-throughput method to identify and characterize meiotic crossovers from haploid testis nuclei.
View Article and Find Full Text PDFBioresour Technol
September 2025
State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; International Joint Laboratory on Fo
Recombinant proteins have been widely applied in the food, biomedical, and scientific fields. Prokaryotic expression systems are preferred platforms for recombinant protein production due to their rapid growth and high protein yields. Nevertheless, disparities between recombinant expression environment and native physiological conditions frequently result in protein misfolding, leading to aggregation into non-functional inclusion bodies or proteolytic degradation.
View Article and Find Full Text PDFNeurology
September 2025
Department of Physical Medicine and Rehabilitation, Spaulding Rehabilitation Hospital, Charlestown, MA.
Background And Objectives: Moderate-to-severe traumatic brain injury (TBI) can result in disability across physical, social, and cognitive domains. The Glasgow Outcome Scale-Extended (GOSE), which evaluates global function, is the most frequently used TBI outcome measure. However, the GOSE may not capture all domains of recovery.
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