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Lipopeptide biosurfactants are surface active biomolecules that are produced by a variety of microorganisms. Microbial lipopeptides have gained the interest of microbiologists, chemists and biochemists for their high biodiversity as well as efficient action, low toxicity and good biodegradability in comparison to synthetic counterparts. In this report, we review methods for the production, isolation and screening, purification and structural characterization of microbial lipopeptides. Several techniques are currently available for each step, and we describe the most commonly utilized and recently developed techniques in this review. Investigations on lipopeptide biosurfactants in natural products require efficient isolation techniques for the characterization and evaluation of chemical and biological properties. A combination of chromatographic and spectroscopic techniques offer opportunities for a better characterization of lipopeptide structures, which in turn can lead to the application of lipopeptides in food, pharmaceutical, cosmetics, agricultural and bioremediation industries.
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http://dx.doi.org/10.3109/07388551.2016.1163324 | DOI Listing |
J Colloid Interface Sci
August 2025
Faculty of Medicine, Pharmacy and Prevention, Department of Life Sciences and Biotechnology, University of Ferrara, Via L. Borsari 46, 44121 Ferrara, Italy. Electronic address:
Titanium dioxide nanoparticles (TiO NPs) are well suited for cosmetics and polymer films because they efficiently absorb UV light while remaining transparent to visible light. Their widespread use requires strategies for managing potential human and environmental risks. Implementing the Safe and Sustainable by Design (SSbD) methodology to advanced chemicals and materials is a major global challenge and a concept that is included in several EU research projects.
View Article and Find Full Text PDFNaunyn Schmiedebergs Arch Pharmacol
September 2025
Department of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.
Biosurfactants, amphiphilic molecules produced by microorganisms, are emerging as promising next-generation antimicrobials due to their diverse structures and mechanisms of action. This review explores the potential of biosurfactants in combating antimicrobial resistance (AMR) and their applications across various fields. We discuss the classification of biosurfactants, including glycolipids, lipopeptides, and phospholipids, highlighting their unique structural features and microbial origins.
View Article and Find Full Text PDFJ Microbiol Biotechnol
August 2025
College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, P.R. China.
Biosurfactants, amphiphilic secondary metabolites mainly produced by microorganisms, can be categorized into five groups according to their chemical structure and source: glycolipids, lipopeptides, phospholipids, polymeric biosurfactants, and particulate surfactants. The hydrophobic segments of biosurfactants typically contain fatty acids of varying chain lengths, while their hydrophilic portions display a wide range of diversity. Diverse biosurfactants have distinct metabolic pathways.
View Article and Find Full Text PDFBiochimie
August 2025
Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia; Center of Molecular and Cellular Biology, Moscow, Russia. Electronic address:
The biosurfactant surfactin is a cyclic lipopeptide produced by different representatives of the genus Bacillus. It has a large surface activity and is considered as a prospective biodegradable detergent. In the present study, we investigated the impact of non-coding 6S-1 RNA - a global transcription regulator in bacteria - on surfactin biosynthesis in the undomesticated B.
View Article and Find Full Text PDFJ Hazard Mater
September 2025
Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.
Isolating microorganisms from oil spill-contaminated environments is essential for advancing bioremediation strategies and discovering novel bioprocesses for hydrocarbon degradation. In this study, we report the isolation of a novel strain, Rhodococcus erythropolis LP27217, from Pertusillo Lake (Italy) on february 2017. Water samples were collected during an oil spill event and microbial community was previously characterized using 16S rRNA gene-targeted metagenomic analysis and functional prediction.
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