98%
921
2 minutes
20
Core Facilities (CF) for advanced light microscopy (ALM) have become indispensable support units for research in the life sciences. Their organizational structure and technical characteristics are quite diverse, although the tasks they pursue and the services they offer are similar. Therefore, throughout Europe, scientists from ALM-CFs are forming networks to promote interactions and discuss best practice models. Here, we present recommendations for ALM-CF operations elaborated by the workgroups of the German network of ALM-CFs, German Bio-Imaging (GerBI). We address technical aspects of CF planning and instrument maintainance, give advice on the organization and management of an ALM-CF, propose a scheme for the training of CF users, and provide an overview of current resources for image processing and analysis. Further, we elaborate on the new challenges and opportunities for professional development and careers created by CFs. While some information specifically refers to the German academic system, most of the content of this article is of general interest for CFs in the life sciences. Microsc. Res. Tech. 79:463-479, 2016. © 2016 THE AUTHORS MICROSCOPY RESEARCH AND TECHNIQUE PUBLISHED BY WILEY PERIODICALS, INC.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5071710 | PMC |
http://dx.doi.org/10.1002/jemt.22648 | DOI Listing |
Curr Opin Neurol
October 2025
Neuromuscular Diseases Unit, Department of Neurology, IR SANT PAU, Hospital de la Santa Creu i Sant Pau, CIBERER, Barcelona, Spain.
Purpose Of Review: Autoimmune nodopathies (AN) are a recognized distinct group of immune-mediated peripheral neuropathies with unique immunopathological features and therapeutic implications. This review synthesizes recent advances in their pathogenesis, diagnosis, and management, which have refined their clinical classification and informed targeted treatment strategies.
Recent Findings: AN are characterized by autoantibodies targeting surface proteins in the nodal-paranodal area (anti-contactin-1, anti-contactin-associated protein 1, anti-neurofascin-155, anti-pan-neurofascin), predominantly of IgG4 subclass.
J Cosmet Dermatol
September 2025
Department of Dermatology, Nantong Third People's Hospital, Affiliated Nantong Hospital 3 of Nantong University, Nantong, China.
Purpose: To evaluate the efficacy and underlying mechanism of advanced optimal pulse technology intense pulsed light (AOPT) in low-energy triple-pulse long-width mode (AOPT-LTL) for melasma treatment.
Methods: An in vivo guinea pig model of melasma was established through progesterone injection and ultraviolet B radiation. Three sessions of AOPT-LTL treatment were performed weekly.
Nanoscale
September 2025
College of Physics, Hebei Normal University, Shijiazhuang 050024, China.
MoSe nanosheet/Si heterojunction photodetectors were fabricated by a mechanical exfoliation method, and their electrical and optical properties at different temperatures were investigated. It was found that the MoSe nanosheet/Si heterojunction device exhibited excellent rectification characteristics at room temperature, and the rectification ratio gradually decreased with the decrease of temperature. The temperature-dependent electrical properties of the MoSe/Si heterojunction device were actually caused by the inhomogeneity of the potential barrier.
View Article and Find Full Text PDFChem Soc Rev
September 2025
Department of Chemistry and Biochemistry, UCSD-CNRS Joint Research Laboratory (IRL3555), University of California, San Diego, La Jolla, CA 92093-0358, USA.
N-Heterocyclic carbenes (NHCs) hold a unique significance in organometallic catalysis and are powerful organocatalysts for a variety of organic transformations involving crucial intermediates such as Breslow intermediates (BIs), deprotonated BIs (BI-s), ketyl radicals (KRs), and acyl azoliums (AAs). To address the remaining challenges facing reactions catalyzed by NHCs, non-classical stable carbenes, namely 1,2,3-triazolylidenes (MICs), cousins of NHCs, have shown great potential. MICs share similar features with typical NHCs but possess unique characteristics, such as enhanced σ-donor ability and absence of dimerization.
View Article and Find Full Text PDFPhys Chem Chem Phys
September 2025
School of Materials Science and Engineering, Changchun University of Science and Technology Changchun, 130022, Jilin, People's Republic of China.
The synergistic effect of various ions with optical properties is an important method to regulate the Er ion upconversion luminescence process. However, the energy processes between them are complicated and difficult to separate, and it is challenging to clarify the results of each process when multiple ions are co-doped. Herein, a series of NaYF:Er were synthesized by the low-temperature combustion method, and the luminescence color of Er ions was modulated by doping Yb ions and Tm ions.
View Article and Find Full Text PDF