Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Glycosylation plays an important role in ensuring the proper structure and function of most biotherapeutic proteins. Even small changes in glycan composition, structure, or location can have a drastic impact on drug safety and efficacy. Recently, glycosylation has become the subject of increased focus as biopharmaceutical companies rush to create not only biosimilars, but also biobetters based on existing biotherapeutic proteins. Against this backdrop of ongoing biopharmaceutical innovation, updated methods for accurate and detailed analysis of protein glycosylation are critical for biopharmaceutical companies and government regulatory agencies alike. This review summarizes current methods of characterizing biopharmaceutical glycosylation, including compositional mass profiling, isomer-specific profiling and structural elucidation by MS and hyphenated techniques.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4155/bio.16.20 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ni-Catalyzed and Pentafluorobenzoate-Leaving-Group-Enabled Stereoselective -Aryl Glycosylation.
Org Lett
September 2025
College of Chemistry and Chemical Engineering and Luoyang Key Laboratory of Green Synthesis and Photofunctional Materials, Luoyang Normal University, Luoyang, Henan 471934, China.
Inspired by the excellent stereoinduction of palladium catalytic glycosylation with glycals via an inner-sphere pathway, a nickel-catalyzed, stereoselective -aryl glycosylation has been developed for glucals bearing a pentafluorobenzoate (PFB) group at the C3 position. The extremely electron-deficient nature of PFB not only endows stronger activity compared to the traditional leaving groups but also functions as an orientation group, presumably through the strong π-π interactions with the bipyridine ligand coordinated to the nickel center, thereby enabling the β-selective formation of a -aryl glycosidic bond with aryl iodides as glycosyl acceptors under mild conditions. This method features a broad substrate scope, high efficiency, and scalability, providing a general solution to the synthesis of challenging β--glycosides.
View Article and Find Full Text PDFCyanidin 3-O-glucoside and other anthocyanins affect enniatins production in Fusarium avenaceum.
Fungal Biol
October 2025
University of Tuscia, Department of Agriculture and Forest Sciences (DAFNE), Via San Camillo de Lellis SNC, Viterbo, Italy.
Fusarium Head Blight (FHB), caused by various Fusarium species, is a major threat to global cereal production. F. avenaceum is an important FHB pathogen producing enniatin mycotoxins.
View Article and Find Full Text PDFMultiple roles and mechanisms of MUC6 in cancer (Review).
Int J Mol Med
November 2025
Department of General Practice, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
Mucin 6 (MUC6), primarily expressed in the gastrointestinal (GI) epithelium, is a member of the mucin family characterized by a protein backbone with extensive glycosylation, playing a crucial role in preserving epithelial barrier integrity. Accumulating evidence indicates that MUC6 glycosylation contributes significantly to cancer development, diagnosis, therapy and prognosis‑particularly in GI malignancies such as gastric, pancreatic and colorectal cancers. In the present review, current findings on the multifaceted roles of MUC6 across various cancers were comprehensively summarize.
View Article and Find Full Text PDFCorrelation between thyroid sensitivity indices and bone metabolism in newly diagnosed middle aged and elderly patients with type 2 diabetes mellitus with normal thyroid function.
BMC Endocr Disord
September 2025
Department of Endocrinology and Metabolism, Jiangxi Medical College, The Second Affiliated Hospital, Nanchang University, Nanchang City, 330006, Jiangxi Province, China.
Objective: To investigate the association between thyroid hormone sensitivity indices and bone metabolism markers in newly diagnosed middle-aged and elderly type 2 diabetes mellitus (T2DM) patients with normal thyroid function.
Method: We retrospectively analyzed 350 newly diagnosed T2DM patients (≥ 45 years), stratified by bone mineral density into Group A (normal bone density group) and Group B (low bone mass and osteoporosis group). General data and clinical biochemical parameters were collected: free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), 25-hydroxyvitamin D (25(OH)D), parathyroid hormone (PTH), osteocalcin (OC), bone-specific alkaline phosphatase (BALP), serum calcium (Ca), serum phosphorus (P), fasting plasma glucose (FPG), glycosylated hemoglobinA1c (HbA1c), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), serum creatinine (SCr), serum uric acid (SUA), and estimated Glomerular Filtration Rate (eGFR).
CRISPR/Cas9-Mediated Knockouts of the ALG3 and GNTI in N. benthamiana and Their Application to Pharmaceutical Production.
Plant Biotechnol J
September 2025
International Center for Biotechnology, University of Osaka, Osaka, Japan.
N-Glycosylation critically influences the efficacy, safety and pharmacokinetic properties of biopharmaceuticals. Plant expression platforms offer multiple advantages for the production of N-glycosylated proteins, but their use is impeded by the presence of plant-specific N-glycan epitopes, which raise concerns of possible immunogenicity to humans. In this study, N-glycoengineered Nicotiana benthamiana plants that produce more homogeneous N-glycans without plant-specific epitopes were generated using multiplex CRISPR/Cas9 genome editing.
View Article and Find Full Text PDF