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Objective: To screen optimal Inonotus obliquus strains that is suitable to cultivation, extracting effective components on industrialized production, genetics and breeding.
Methods: Inonotus obliquus mycelia from different countries were used. High quality strain was screened by comparing the antagonism between Inonotus obliquus and penicillium. The contents of crude fat, crude ash, crude protein and crude fiber were analyzed.
Results: It was showed that antagonism ability of Finnish birch was the strongest, its mycelia diameter was up to 53. 27 mm. It was also showed that nutritional ingredient was abundant in artificial cultured Inonotus obliquus strains. The contents of crude fat and crude protein in Finnish birch were higher, which was 1. 93% and 20. 23%, respectively. The contents of crude fat and crude protein in NBRC8681 strains were lower, which was 1. 55% and 19. 85%, respectively. The content of crude ash of JL04 strains was higher(8. 76%). The content of crude ash of NBRC8681 strains was lower(6. 8%). Crude fiber content was higher in HLJ01 strains, its content reached to 45. 09%. The least content appeared in MAFF420101 strains, whose crude fiber content was only 38. 27%.
Conclusion: The strains of Finnish birch, HLJ01 and JL04 are suitable to exploitation.
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Bioorg Chem
August 2025
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Faculty of Medicine, Tianjin University, Tianjin 300072, PR China. Electronic address:
Inonotus obliquus is a medicinal fungus that has been valued for its anti-diabetic properties in China for thousands of years. Inotodiol is a characteristic triterpenoid isolated from Inonotus obliquus. The inactivation effect and mechanism of α-glucosidase induced by inotodiol were investigated using fluorescence, FTIR, CD spectroscopy, and molecular dynamics simulation, and the glycemic regulation of inotodiol was studied on insulin-resistant HepG2 cells.
View Article and Find Full Text PDFApoptosis
August 2025
Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute-Cairo University, Cairo, Egypt.
Chaga mushroom (Inonotus obliquus) exhibits cytotoxic effects against breast cancer cells. Mycosynthesized nanoparticles, owing to their biodegradability, biocompatibility, and low toxicity, present a promising therapeutic approach. This study explored the cytotoxic potential of gold nanoparticles synthesized using Chaga mushroom extract (AuCh-NPs) combined with Light Emitting Diode (LED) irradiation (530 nm) on human breast cancer (MCF-7) cells, aiming to develop a safe and effective sensitizer for photothermal therapy.
View Article and Find Full Text PDFInt J Mol Sci
August 2025
Food Science and Technology Program, Department of Life Sciences, Beijing Normal-Hong Kong Baptist University, Zhuhai 519087, China.
This study aimed to explore the molecular mechanisms of phytochemicals from Chaga mushroom () against colorectal cancer (CRC) using a combination of network pharmacology, molecular docking, and bioinformatics. Active components and targets of Chaga mushroom and CRC were collected from databases. A drug-compound-target-disease network was constructed, and protein-protein interaction (PPI) analysis was performed to identify core targets.
View Article and Find Full Text PDFPlanta Med
August 2025
National Centre for Natural Products, The University of Mississippi, University, United States.
Inonotus obliquus is widely recognized as the Chaga mushroom. Chaga contains various bioactive compounds, including polysaccharides, triterpenoids, polyphenols, and melanin. To address the characterization and quantitative analysis of triterpenoids and phenolics in Chaga, a multi-analytical approach combining LC-PDA-ELSD and LC-DAD-QToF has been developed.
View Article and Find Full Text PDFPolymers (Basel)
August 2025
Drug Research Center, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Romania.
, a medicinal mushroom valued for its bioactive compounds, has not been previously characterized from Romanian sources. This study presents the first comprehensive chemical and biological screening of , introducing novel polymer-based encapsulation systems to enhance the stability and bioavailability of its bioactive constituents. Two distinct delivery systems were designed to enhance the functionality of extracts: (i) microencapsulation in maltodextrin (MIO) and (ii) a sequential approach involving preparation of silver nanoparticle-loaded (IO-AgNPs), followed by microencapsulation to yield the hybrid MIO-AgNP system.
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