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Recycling counter-current chromatography (CCC) together with step-gradient CCC and medium-pressure liquid chromatography (MPLC) was employed to separate nine anthraquinone compounds from Cassia obtusifolia L. in this study. The results showed that recycling CCC is a powerful tool for compounds that are difficult to separate with common elution mode. CCC was the better option for crude material while MPLC had advantage for the final tuning. The combination of recycling CCC and MPLC could simplify the method exploring process in the separation process. The structures of these compounds were identified according to their mass spectra, by (1)H-NMR and compared with standard compounds.
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http://dx.doi.org/10.1002/jssc.201100535 | DOI Listing |
Microbiol Spectr
September 2025
Faculty of Medicine and Medical Sciences, University of Balamand, Tripoli, Lebanon.
In Gram-negative bacteria, resistance-nodulation-division (RND)-type efflux pumps, particularly AcrAB-TolC, play a critical role in mediating resistance to antimicrobial agents and toxic metabolites, contributing to multidrug resistance. is an entomopathogenic bacterium that has garnered significant interest due to its production of bioactive specialized metabolites with anti-inflammatory, antimicrobial, and scavenger deterrent properties. In previous work, we demonstrated that AcrAB confers self-resistance to stilbenes in TT01.
View Article and Find Full Text PDFCarbohydr Polym
November 2025
Engineering Research Center for Biomedical Materials, Anhui Key Laboratory of Modern Biomanufacturing, School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui Province 230601, PR China. Electronic address:
The primary objective of this study was to develop a nanosuspension based on orthoester compounds (OE) and carboxymethyl chitosan (CMCS) for the combined treatment of tumors. Initially, OE was synthesized as a liquid pharmaceutical excipient. Subsequently, nanoparticles were formulated using CMCS and loaded with mitoxantrone (MIT).
View Article and Find Full Text PDFCurr Pharm Des
August 2025
Department of Food Science, National Taiwan Ocean University, Keelung 202, Taiwan.
Introduction: Traditional Chinese Medicine (TCM) employs various processing methods to enhance the bioactivity of herbs. Rheum palmatum (R. palmatum) is commonly processed to optimize its medicinal properties, yet its antibacterial activity under different processing techniques remains unclear.
View Article and Find Full Text PDFPhytother Res
September 2025
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
MicroRNAs (miRNAs) have emerged as critical regulators in the pathogenesis of diabetes mellitus (DM) and its associated complications via orchestrating key cellular processes. Natural bioactive components such as flavonoids, polysaccharides, saponins, alkaloids, terpenoids, as well as anthraquinones derived from berries, herbs, vegetables, and fishes have been extensively demonstrated to treat or prevent DM and related complications through targeting miRNAs-mediated signaling pathways. Mechanistically, these compounds treat/prevent DM and its complications principally by modulating processes including glucose homeostasis, inflammatory response, oxidative stress, apoptosis, and autophagy, neovascularization, and fibrosis.
View Article and Find Full Text PDFJ Ethnopharmacol
August 2025
Department of Biochemistry, Landmark University, PMB 1001, Omu-Aran, Nigeria; Department of Pathology and Laboratory Medicine, Tulane University School of Medicine, New Orleans, LA, United States.
Ethnopharmacological Relevance: The increase in incidence of male fertility concerns have prompted investigation into Borassus aethiopum (Mart.), a medicinal plant with claimed male sex enhancing property with a bid to scientifically prove or disprove the claim.
Aim Of The Study: This study therefore evaluated the androgenic potential of ethylacetate fraction of Borassus aethiopum hypocotyl (EFBAH) in male animal models using HPLC analysis (with saponin standard) and molecular docking studies.