Separation of anthraquinone compounds from the seed of Cassia obtusifolia L. using recycling counter-current chromatography.

J Sep Sci

State Key Laboratory of Biotherapy, West China Hospital and College of Pharmacy, Sichuan University, Chengdu, Sichuan, P. R. China; State Key Laboratory Breeding Base of Systematic Research Development of Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, S

Published: January 2012


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Recycling counter-current chromatography (CCC) together with step-gradient CCC and medium-pressure liquid chromatography (MPLC) was employed to separate nine anthraquinone compounds from Cassia obtusifolia L. in this study. The results showed that recycling CCC is a powerful tool for compounds that are difficult to separate with common elution mode. CCC was the better option for crude material while MPLC had advantage for the final tuning. The combination of recycling CCC and MPLC could simplify the method exploring process in the separation process. The structures of these compounds were identified according to their mass spectra, by (1)H-NMR and compared with standard compounds.

Download full-text PDF

Source
http://dx.doi.org/10.1002/jssc.201100535DOI Listing

Publication Analysis

Top Keywords

anthraquinone compounds
8
cassia obtusifolia
8
recycling counter-current
8
counter-current chromatography
8
recycling ccc
8
compounds
5
ccc
5
separation anthraquinone
4
compounds seed
4
seed cassia
4

Similar Publications

In Gram-negative bacteria, resistance-nodulation-division (RND)-type efflux pumps, particularly AcrAB-TolC, play a critical role in mediating resistance to antimicrobial agents and toxic metabolites, contributing to multidrug resistance. is an entomopathogenic bacterium that has garnered significant interest due to its production of bioactive specialized metabolites with anti-inflammatory, antimicrobial, and scavenger deterrent properties. In previous work, we demonstrated that AcrAB confers self-resistance to stilbenes in TT01.

View Article and Find Full Text PDF

An injectable nanosuspension based on orthoester and biomimetic carboxymethyl chitosan nanoparticles for chemo/thermo-synergistic tumor therapy.

Carbohydr Polym

November 2025

Engineering Research Center for Biomedical Materials, Anhui Key Laboratory of Modern Biomanufacturing, School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui Province 230601, PR China. Electronic address:

The primary objective of this study was to develop a nanosuspension based on orthoester compounds (OE) and carboxymethyl chitosan (CMCS) for the combined treatment of tumors. Initially, OE was synthesized as a liquid pharmaceutical excipient. Subsequently, nanoparticles were formulated using CMCS and loaded with mitoxantrone (MIT).

View Article and Find Full Text PDF

Introduction: Traditional Chinese Medicine (TCM) employs various processing methods to enhance the bioactivity of herbs. Rheum palmatum (R. palmatum) is commonly processed to optimize its medicinal properties, yet its antibacterial activity under different processing techniques remains unclear.

View Article and Find Full Text PDF

MicroRNAs for Diabetes Management: From Natural Bioactive Compounds to Nucleotide Networks.

Phytother Res

September 2025

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

MicroRNAs (miRNAs) have emerged as critical regulators in the pathogenesis of diabetes mellitus (DM) and its associated complications via orchestrating key cellular processes. Natural bioactive components such as flavonoids, polysaccharides, saponins, alkaloids, terpenoids, as well as anthraquinones derived from berries, herbs, vegetables, and fishes have been extensively demonstrated to treat or prevent DM and related complications through targeting miRNAs-mediated signaling pathways. Mechanistically, these compounds treat/prevent DM and its complications principally by modulating processes including glucose homeostasis, inflammatory response, oxidative stress, apoptosis, and autophagy, neovascularization, and fibrosis.

View Article and Find Full Text PDF

Molecular docking, HPLC phytochemical profiling and androgenic property of ethylacetate fraction of Borassusaethiopum (Mart.) hypocotyl in pre-clinical models.

J Ethnopharmacol

August 2025

Department of Biochemistry, Landmark University, PMB 1001, Omu-Aran, Nigeria; Department of Pathology and Laboratory Medicine, Tulane University School of Medicine, New Orleans, LA, United States.

Ethnopharmacological Relevance: The increase in incidence of male fertility concerns have prompted investigation into Borassus aethiopum (Mart.), a medicinal plant with claimed male sex enhancing property with a bid to scientifically prove or disprove the claim.

Aim Of The Study: This study therefore evaluated the androgenic potential of ethylacetate fraction of Borassus aethiopum hypocotyl (EFBAH) in male animal models using HPLC analysis (with saponin standard) and molecular docking studies.

View Article and Find Full Text PDF