Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The mRNA closed-loop, formed through interactions between the cap structure, poly(A) tail, eIF4E, eIF4G and PAB, features centrally in models of eukaryotic translation initiation, although direct support for its existence in vivo is not well established. Here, we investigated the closed-loop using a combination of mRNP isolation from rapidly cross-linked cells and high-throughput qPCR. Using the interaction between these factors and the opposing ends of mRNAs as a proxy for the closed-loop, we provide evidence that it is prevalent for eIF4E/4G-bound but unexpectedly sparse for PAB1-bound mRNAs, suggesting it primarily occurs during a distinct phase of polysome assembly. We observed mRNA-specific variation in the extent of closed-loop formation, consistent with a role for polysome topology in the control of gene expression.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615164 | PMC |
| http://dx.doi.org/10.1080/15476286.2015.1017242 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dual-Functional Microneedles for In Situ Diagnosis and Biofilm-Targeted Therapy of Diabetic Periodontitis via Biomarker-Responsive Probes and Photothermal NO Nanomotors.
Anal Chem
September 2025
Marshall Laboratory of Biomedical Engineering, Shenzhen Key Laboratory for Nano-Biosensing Technology, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Shenzhen, Guangdong 518060, China.
Chronic periodontitis, a frequent complication of diabetes, is exacerbated by bacterial biofilms that drive progressive periodontal tissue destruction and systemic inflammation. Conventional treatments, utilizing mechanical debridement and systemic antibiotics, often fail to eradicate bacterial biofilms, promote antibiotic resistance, and lack real-time monitoring, leading to suboptimal therapeutic outcomes. Herein, we report a separable bilayer microneedle (MN) patch that enables localized, antibiotic-free, biofilm-targeted therapy and in situ biomarker-based monitoring for the integrated management of chronic periodontitis.
View Article and Find Full Text PDFWe established a theoretical model for the ladder-type EIT laser frequency stabilization method based on the analysis of the system response. By using the method of deriving the density matrix equations, we obtained the density matrix elements of the three-level system and subsequently derived the system's response function. Based on this, we revealed the complex interference mechanism of the modulated probe light in the atomic medium.
View Article and Find Full Text PDFAdvances in the near-infrared Ⅱ for in vivo fluorescence imaging applications: A review.
Talanta
August 2025
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China. Electronic address:
Second near-infrared (NIR-II) fluorescence imaging technology utilizes the wavelength range of 1000-1700 nm, significantly reducing tissue absorption and scattering, and combining high spatial resolution and extremely low spontaneous fluorescence, bringing a breakthrough to biomedical imaging. This review systematically explores the fundamental principles and intrinsic advantages of NIR-II optical imaging. Crucially, it delineates three strategies for probe design: achieving red-shifted emission wavelengths, modulating donor-acceptor electronic structures, and implementing synergistic metal coordination coupled with surface engineering.
View Article and Find Full Text PDFBeyond Structure: Next-Generation Electrophysiological Platforms for Functional Brain Organoids.
Int J Stem Cells
August 2025
Department of Convergence Medicine, College of Medicine, Korea University, Seoul, Korea.
Brain organoids have emerged as transformative models for studying human neurodevelopment, neurological disorders, and personalized therapeutics. Central to their utility is the ability to monitor neural activity with high spatial and temporal resolution. Traditional electrophysiological tools-such as planar microelectrode arrays and patch-clamp techniques-offer limited access to the three-dimensional and dynamic nature of organoid neural networks.
View Article and Find Full Text PDFBrain-computer interfaces as a causal probe for scientific inquiry.
Trends Cogn Sci
July 2025
Department of Electrical and Computer Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA; Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA; Center for the Neural Basis of Cognition, Carnegie Mellon University and University of Pittsburgh, Pi
Establishing causal relationships between neural activity and brain function requires experimental perturbations of neural activity. Many existing perturbation methods modify activity by directly applying external signals to the brain. We review an alternative approach where brain-computer interfaces (BCIs) leverage volitional control of neural activity to manipulate and causally perturb it.
View Article and Find Full Text PDF