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To enhance the marker density in the "QTL-hotspot" region, harboring several QTLs for drought tolerance-related traits identified on linkage group 04 (CaLG04) in chickpea recombinant inbred line (RIL) mapping population ICC 4958 × ICC 1882, a genotyping-by-sequencing approach was adopted. In total, 6.24 Gb data from ICC 4958, 5.65 Gb data from ICC 1882 and 59.03 Gb data from RILs were generated, which identified 828 novel single-nucleotide polymorphisms (SNPs) for genetic mapping. Together with these new markers, a high-density intra-specific genetic map was developed that comprised 1,007 marker loci spanning a distance of 727.29 cM. QTL analysis using the extended genetic map along with precise phenotyping data for 20 traits collected over one to seven seasons identified 49 SNP markers in the "QTL-hotspot" region. These efforts have refined the "QTL-hotspot" region to 14 cM. In total, 164 main-effect QTLs including 24 novel QTLs were identified. In addition, 49 SNPs integrated in the "QTL-hotspot" region were converted into cleaved amplified polymorphic sequence (CAPS) and derived CAPS (dCAPS) markers which can be used in marker-assisted breeding.
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http://dx.doi.org/10.1007/s00438-014-0932-3 | DOI Listing |
Sci Rep
July 2025
Department of Biology, Faculty of Mathematics and Natural Science, IPB University, Kampus IPB Dramaga, Bogor, 16680, Indonesia.
Rice is a staple crop worldwide, with seed traits such as size, shape, weight, and color playing crucial roles in agricultural productivity and consumer preferences. Despite significant progress, the genetic basis underlying the variation in hulled and unhulled seed grain traits remains partially unexplored. This study presents a comprehensive analysis combining GWAS and QTL mapping to dissect the genetic architecture of hulled and unhulled seed characteristics in rice.
View Article and Find Full Text PDFPlant Biotechnol J
September 2025
WA State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, Food Futures Institute, Murdoch University, Murdoch, Western Australia, Australia.
Chickpea (Cicer arietinum L.) is an important legume crop that has been subjected to intensive breeding, resulting in limited genetic diversity. Australia is the world's second largest producer and the leading exporter of chickpea; the genomic architecture of its cultivars remains largely unexplored.
View Article and Find Full Text PDFPlants (Basel)
April 2025
Henan Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences, Henan Key Laboratory of Wheat Biology, National Engineering Laboratory of Wheat Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Zhengzhou 450002, China.
Nickel (Ni) pollution poses significant threats to human health and crop development through the food chain. This study aimed to identify the novel genomic regions and superior alleles associated with Ni accumulation in wheat ( L.) grains using genome-wide association analysis (GWAS) with a diversity panel of 207 bread wheat varieties.
View Article and Find Full Text PDFFront Plant Sci
January 2025
International Rice Research Institute, Los Baños, Philippines.
Water scarcity and labor shortage pose significant challenges in rice farming. Direct-seeded rice (DSR) is an efficient method that conserves water, reduces labor costs, and allows for full mechanization of cultivation. However, variable planting depth in undulated field leading to deep/shallow sowing of rice seeds during mechanical sowing presents a major hurdle, as existing varieties lack tolerance to deep sowing.
View Article and Find Full Text PDFBlood
February 2025
Department of Pathology, Carter Immunology Center, University of Virginia School of Medicine, Charlottesville, VA.
Red blood cell (RBC) metabolism regulates hemolysis during aging in vivo and in the blood bank. However, the genetic underpinnings of RBC metabolic heterogeneity and extravascular hemolysis at population scale are incompletely understood. On the basis of the breeding of 8 founder strains with extreme genetic diversity, the Jackson Laboratory diversity outbred population can capture the impact of genetic heterogeneity in like manner to population-based studies.
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