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This paper introduces a nanopipette combined with a quartz tuning fork-atomic force microscope system (nanopipette/QTF-AFM), and describes experimental and theoretical investigations of the nanoscale materials used. The system offers several advantages over conventional cantilever-based AFM and QTF-AFM systems, including simple control of the quality factor based on the contact position of the QTF, easy variation of the effective tip diameter, electrical detection, on-demand delivery and patterning of various solutions, and in situ surface characterization after patterning. This tool enables nanoscale liquid delivery and nanofabrication processes without damaging the apex of the tip in various environments, and also offers force spectroscopy and microscopy capabilities.
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http://dx.doi.org/10.1063/1.4866656 | DOI Listing |
Anal Chem
August 2025
UCAM-SENS, Universidad Católica San Antonio de Murcia, UCAM HiTech, Avda. Andres Hernandez Ros 1, 30107 Murcia, Spain.
Nanopipettes with carbon-coated inner surfaces (carbon nanopipettes, CNPs) have attracted considerable attention due to their exceptional sensitivity and potential in electroanalytical applications. The nanoconfinement of the sample solution within the CNP facilitates a thin-layer electrochemical regime, in which ion and electron transferences are inherently coupled. This feature allows exhaustive oxidation/reduction of certain analytes within typical electroanalytical time scales, offering unprecedented opportunities for nanoscale sensing.
View Article and Find Full Text PDFInt J Biol Macromol
May 2025
Institut Européen des Membranes, UMR5635 University of Montpellier ENSCM CNRS, Place Eugène Bataillon, 34095 Montpellier cedex 5, France. Electronic address:
Parkinson's disease (PD) is a prevalent neurodegenerative disorder characterized by the abnormal aggregation of α-synuclein. This study investigated the impact of Cu ions on α-synuclein aggregation in oligomer distribution using two single-molecule techniques. The aggregation of α-synuclein monomers with and without Cu revealed that Cu accelerated the formation of ThT-positive β-sheet structured aggregates.
View Article and Find Full Text PDFNano Lett
March 2025
Department of Electrical Engineering, Graduate School of Engineering, Nagoya University, Aichi 464-8601, Japan.
Because endogenous extracellular vesicles are involved in important physiological functions, various techniques have been developed for their isolation and evaluation. However, methods for evaluating endogenous vesicles within cells are limited. This study presents a technique for the direct extraction and evaluation of intraluminal vesicles (ILVs).
View Article and Find Full Text PDFBiosensors (Basel)
December 2024
Research Laboratory of Biophysics, National University of Science and Technology "MISIS", 119049 Moscow, Russia.
Nanopipettes, as a class of solid-state nanopores, have evolved into universal tools in biomedicine for the detection of biomarkers and different biological analytes. Nanopipette-based methods combine high sensitivity, selectivity, single-molecule resolution, and multifunctionality. The features have significantly expanded interest in their applications for the biomolecular detection, imaging, and molecular diagnostics of real samples.
View Article and Find Full Text PDFAdv Sci (Weinh)
October 2024
Institut Européen des Membranes, UMR5635 University of Montpellier ENCSM CNRS, Place Eugène Bataillon, Montpellier, 34095, France.
Understanding the mechanisms underlying amyloid-β (Aβ) aggregation is pivotal in the context of Alzheimer's disease. This study aims to elucidate the secondary nucleation process of Aβ42 peptides by combining experimental and computational methods. Using a newly developed nanopipette-based amyloid seeding and translocation assay, confocal fluorescence spectroscopy, and molecular dynamics simulations, the influence of the seed properties on Aβ aggregation is investigated.
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