Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
To improve the efficiency of producing cloned pigs, we investigated the influence of the number of transferred embryos, the culturing interval between nuclear transfer (NT) and embryo transfer, and the transfer pattern (single oviduct or double oviduct) on cloning efficiency. The results demonstrated that transfer of either 150-200 or more than 200NT embryos compared to transfer of 100-150 embryos resulted in a significantly higher pregnancy rate (48 ± 16, 50 ± 16 vs. 29 ± 5%, p<0.05) and average litter size (4.1 ± 2.3, 7 ± 3.6 vs. 2.5 ± 0.5). In vitro culture of reconstructed embryos for a longer time (40 h vs. 20 h) resulted in higher (p<0.05) pregnancy rate (44 ± 9 vs. 31 ± 3%) and delivery rate (44 ± 9 vs. 25 ± 9%). Furthermore, double oviductal transfer dramatically increased pregnancy rate (83 ± 6 vs. 27+8%, p<0.05), delivery rate (75 ± 2 vs. 27+8%, p<0.05) and average litter size (6.5 ± 2.8 vs. 2.6 ± 1.2) compared to single oviductal transfer. Our study demonstrated that an improvement in pig cloning efficiency is achieved by adjusting the number and in vitro culture time of reconstructed embryos as well as the embryo transfer pattern.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.anireprosci.2013.10.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Serum progesterone variability on embryo transfer day in hormone replacement therapy cycles using intramuscular injections during frozen embryo transfers.
J Assist Reprod Genet
September 2025
Bahçeci Fulya IVF Center, Infertility Clinic, Istanbul, Turkey.
Purpose: To assess the intra-individual variability of serum progesterone (P) levels on embryo transfer (ET) day, when the same dose of intramuscular progesterone (IM-P) was used in two consecutive hormone replacement therapy (HRT) frozen embryo transfer (FET) cycles.
Methods: A total of 75 patients undergoing two consecutive HRT-FET cycles in one year performed at Bahceci Ankara IVF Center between November 2019 and February 2022 were retrospectively analyzed. Serum P levels were measured at the 117th-119th hours of support by a single laboratory.
Communities of plasmids as strategies for antimicrobial resistance gene survival in wastewater treatment plant effluent.
NPJ Antimicrob Resist
September 2025
Antimicrobial Resistance & Microbiome Research Group, Department of Biology, The Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co, Kildare, Ireland.
Plasmids facilitate antimicrobial resistance (AMR) gene spread via horizontal gene transfer, yet the mobility of genes in wastewater treatment plant (WWTP) resistomes remains unclear. We sequenced 173 circularised plasmids transferred from WWTP effluent into Escherichia coli and characterised their genetic content. Multiple multidrug-resistant plasmids were identified, with a significant number of mega-plasmids (>100 kb).
View Article and Find Full Text PDFComputational fluid dynamics (CFD) modelling of liquid embolic agents (Onyx) used in brain arteriovenous malformation (AVM) treatment to predict the distal penetration behavior.
Med Eng Phys
October 2025
Ansys Inc., Houston, TX 77094, USA.
Introduction: Benchtop and animal models have traditionally been used to study the propagation of Onyx Liquid Embolic Systems (Onyx) used in the treatment of brain arteriovenous malformations (AVM). However, such models are costly, do not provide sufficient detail to elucidate how variations in Onyx viscosity alter flow dynamics, and rely on some trial-and-error, resulting in elongated timelines for product development.
Objectives: The goal of this study was to leverage Computational Fluid Dynamics (CFD) simulations to predict the behavior of different Onyx formulations.
Development and Implementation of a New Process to Improve Safety of Urgent Direct Admissions.
Hosp Pediatr
September 2025
Children's Hospital of Philadelphia, Philadelphia, Pennsylvania.
Background: Direct admission can help reduce emergency department crowding, improve patient satisfaction, and decrease costs, yet there is opportunity to improve standardized processes to do so safely and efficiently. We designed and implemented a new process for urgent direct admission (UDA) at our children's hospital with the SMART (specific, measurable, achievable, relevant, time-bound) aim to increase the number of UDAs between transfer to an intensive care unit (ICU) within 12 hours from direct admission by 50% in 12 months.
Methods: We compared unanticipated ICU transfers within 12 hours of admission (outcome) before and after implementing a standardized UDA process.
Lignin-mediated AgCoOS bimetal oxysulfide catalysts for highly efficient catalytic reduction of organic and Cr(VI) pollutants in the dark.
Int J Biol Macromol
September 2025
College of Materials Engineering, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address:
Lignin, a negatively charged, three-dimensional natural biopolymer, serves as an ideal support for metal catalysts due to its abundant functional groups and tunable chemical properties, which enable strong metal coordination and effective immobilization. Herein, we demonstrate a lignin-mediated Co/O co-doped AgS, symbolized as L-AgCoOS, bimetal oxysulfide catalyst via a facile hydrolysis method for the efficient reduction of toxic phenolic compounds (4-nitrophenol, 4-NP), organic dyes (methyl orange (MO), methylene blue (MB), rhodamine B (RhB), and heavy metal ions Cr(VI)) under dark conditions. Lignin, used to immobilize catalysts, also contributes to increasing the number of active catalytic sites and enhancing catalytic activity.
View Article and Find Full Text PDF