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The objective of this work was to determine some of the indicators associated to shelf life, hygiene, process and storage conditions for some of custard apple, mango and passion fruit pulps distributed by the main supermarket chains of the Metropolitan Area of San José, Costa Rica, as well as to examine the potential presence of Listeria monocytogenes in them. Sixty fruit pulp samples were analyzed. Tests included pH determination, total aerobic plate count, yeasts and mold count, lactic bacteria count, total and fecal most probable number and the presence/absence of Listeria monocytogenes in 25 g of the product. Fruit pulp's pH ranged between 3,1 and 3,9, and the microbiological counts obtained were relatively low except for one industry. None of the samples analyzed presented total or fecal coliforms. The presence of Listeria monocytogenes was confirmed in three samples, all of them coming from industry C. Low microbiological counts obtained may be due to the addition of preserving substances and to the pasteurization of some of the products; lack of these two elements may allow the presence of dangerous bacteria such as Listeria monocytogenes.
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Crit Rev Immunol
January 2025
Department of Pharmacy, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Dist. Medchal,500078, Telangana State, India.
Caseinolytic protease P (ClpP) is a highly conserved serine protease that plays a pivotal role in protein homeostasis and quality control in bacteria, mitochondria of mammalian cells, and plant chloroplasts. As the proteolytic core of the ATP-dependent Clp protease complex, ClpP partners with regulatory ATPases (e.g.
View Article and Find Full Text PDFJ Appl Microbiol
September 2025
Graduate Institute of Medical Sciences, National Defense Medical University, Taipei City 114201, Taiwan (R.O.C.).
Aims: This study aims to develop and evaluate a rapid and high-multiplex pathogen detection method for clinical and food specimens to address the ongoing public health threat of foodborne infections and the limitations of conventional culture-based diagnostics.
Methods And Results: The foodborne bacteria (FBB) assay integrates multiplex PCR, T7 exonuclease hydrolysis, and a suspension bead array to simultaneously detect 16 genes from 13 major foodborne bacteria. Analytical performance was evaluated using reference strains, while diagnostic performance was assessed using clinical and food samples.
Food Sci Biotechnol
October 2025
Department of Food Science & Technology, Chungnam National University, Daejeon, 34134 Republic of Korea.
poses significant challenges in the food industry due to its resistance in harsh environments and its ability to form biofilms. Endolysins represent a promising solution for controlling in the food industry, with potential applications during both production and storage. This review discusses various endolysins that effectively inhibit , emphasizing their optimal conditions and potential uses in food products.
View Article and Find Full Text PDFFood Sci Nutr
September 2025
Chemistry Laboratory, Kouba Higher Normal School, Algiers Kouba Algeria.
The objective of this investigation was to assess the biological properties of the leaf's aqueous extract of (PaAE), which is used in conventional medicine for therapeutic purposes of gastric ulcers and abdominal diseases. The content of phenolic and flavonoidic compounds was quantitatively estimated using colorimetric methods. The phenolic component profile was also evaluated using LC-MS/MS.
View Article and Find Full Text PDFEnviron Microbiol
September 2025
Listeria: Biology and Infection Research Group (LisBio), Valencia, Spain.
Listeria monocytogenes is a saprophytic bacterium and a foodborne pathogen of humans and animals. Little is known about its distribution and genetic diversity across different environments within the same geographical region. We conducted a large-scale longitudinal study in southeastern Spain monitoring Listeria spp.
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