Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Reconstructing Evolutionary Adaptive Paths (REAP) is one of several methods to improve enzyme -functionality. This approach incorporates computational and theoretical aspects of protein engineering to create a focused library of protein variety with a high degree of functionality. In contrast to other -techniques like DNA shuffling, REAP allows a library to have diverse functionality among relatively few variants. REAP is a low-throughput method which takes advantage of natural selection and uses ancestral protein sequences to direct gene mutations, thereby creating a library with a high density of viable proteins. These proteins must then be assayed to characterize their functionality to identify which variants have the desired traits such as acid stability or thermostability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-62703-293-3_8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evolution and structural diversity of the MotAB stator: insights into the origins of bacterial flagellar motility.
mBio
September 2025
School of Biological Sciences, University of Auckland, Auckland, New Zealand.
The rotation of the bacterial flagellum is powered by the MotAB stator complex, which converts ion flux into torque. Despite its central role in flagellar function, the evolutionary origin and structural diversity of this system remain poorly understood. Here, we present the first comprehensive phylogenetic and structural characterization of MotAB and its closest non-flagellar homologs.
View Article and Find Full Text PDFHuman-like malformations in anole lizards: Potential cases of "hopeful monsters" resembling chameleon morphology.
J Anat
September 2025
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland.
Vertebrates exhibit remarkable morphological diversity, with the head representing an exceptionally complex anatomical structure shaped by adaptations to feeding ecology, brain size, and sensory organ specialization. Proper fusion of facial prominences and the coordinated growth of the skull and brain are essential for normal craniofacial development in vertebrates, including humans. Disruptions in these processes, whether due to gene mutations or external factors, can result in craniofacial malformations.
View Article and Find Full Text PDFConvergent evolution of -facilitated root nodule symbiosis.
Proc Natl Acad Sci U S A
September 2025
Florida Museum of Natural History, University of Florida, Gainesville, FL 32611.
The origin and phylogenetic distribution of symbiotic associations between nodulating angiosperms and nitrogen-fixing bacteria have long intrigued biologists. Recent comparative evolutionary analyses have yielded alternative hypotheses: a multistep pathway of independent gains and losses of root nodule symbiosis vs. a single gain followed by numerous losses.
View Article and Find Full Text PDFTaxonomy, phylogeny, and biogeography of the Oriental subfamily Perittopinae China & Usinger, 1949 (Hemiptera: Heteroptera: Veliidae).
Zool Res
September 2025
Institute of Entomology, College of Life Sciences, Nankai University, Tianjin 300071, China. E-mail:
The collision of the Indian and Eurasian plates during the Eocene represents a major tectonic shift that significantly altered biotic dynamics and promoted species diversification across the Oriental region. To explain the diversification of taxa from the Indian subcontinent into Southeast Asia, two principal hypotheses have been proposed: the "Biotic-ferry" and "Step-stone" models. The subfamily Perittopinae, a lineage of semi-aquatic bugs comprising a single genus and 20 extant species, provides an ideal system for testing these hypotheses due to its disjunct distribution spanning the Indian subcontinent and Southeast Asia.
View Article and Find Full Text PDFTaxonomic revision of the critically endangered big-headed turtles (Reptilia: Testudines: Platysternidae Gray, 1869), with description of a new species.
Zool Res
September 2025
College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China. E-mail:
The big-headed turtle ( ), currently the only extant member of the genus and the family Platysternidae, has undergone severe population declines driven by poaching, illegal trade, and habitat loss, leading to its classification as Critically Endangered (CR) by the International Union for Conservation of Nature (IUCN). Despite its conservation status, persistent taxonomic ambiguities and unresolved phylogenetic relationships have hindered effective protection and management. This study integrated evidence from genome-wide single nucleotide polymorphisms (SNPs), mitochondrial DNA sequences ( , ), and morphological data to reconstruct the phylogeny and phylogeography of and revise its taxonomy.
View Article and Find Full Text PDF