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In this paper, we propose a novel, magnetically driven microrobot equipped with a frame structure to measure the effects of stimulating aquatic microorganisms. The design and fabrication of the force-sensing structure with a displacement magnification mechanism based on beam deformation are described. The microrobot is composed of a Si-Ni hybrid structure constructed using micro-electro-mechanical system (MEMS) technologies. The microrobots with 5 μm-wide force sensors are actuated in a microfluidic chip by permanent magnets so that they can locally stimulate the microorganisms with the desired force within the stable environment of the closed microchip. They afford centimetre-order mobility (untethered drive) and millinewton-order forces (high power) as well as force-sensing. Finally, we apply the developed microrobots for the quantitative evaluation of the stimuation of Pleurosira laevis (P. laevis) and determine the relationship between the applied force and the response of a single cell.
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http://dx.doi.org/10.1039/c2lc41190c | DOI Listing |
Curr Microbiol
September 2025
Laboratory for Structural Analysis of Biomacromolecules, Kazan Scientific Center of Russian Academy of Science, Kazan, Russia.
Phosphorylated structural analogs of Benzalkonium Chloride-diisopropoxyphosphorylmethane (dimethyldodecylammonium) bromide 1 (phosphorylated quaternary ammonium salt) and isopropoxyphosphorylmethane (dimethylalkylammonium) 2 (phosphorylated betaine) were synthesized. The structure of compound 1 was confirmed by single crystal X-ray diffraction study. The antibacterial, antifungal, and ecotoxicological profiles of the synthesized compounds were evaluated against aquatic organisms and flowering plants.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2025
The Biorobotics Institute, Scuola Superiore Sant'Anna, Pisa 56025, Italy.
Aquatic microorganisms typically inhabit a heterogeneous resource landscape, composed of localized and transient patches. To effectively exploit these resources, they have evolved a wide range of feeding strategies that combine chemotactic motility with active feeding flows. However, there is a notable lack of experimental studies that examine how these active flows shape resource fields to optimize feeding.
View Article and Find Full Text PDFAppl Environ Microbiol
September 2025
Department of Environmental Microbiology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, Dübendorf, Switzerland.
Unlabelled: The genus includes opportunistic pathogens inhabiting engineered aquatic ecosystems, where managing their presence and abundance is crucial for public health. In these environments, interact positively or negatively with multiple members of the microbial communities. Here, we identified bacteria and compounds with -antagonistic properties.
View Article and Find Full Text PDFFish Shellfish Immunol
September 2025
Xianghu Laboratory, Hangzhou, Zhejiang 311231, China. Electronic address:
Background: The appearance and cross-border dissemination of numerous pathogenic microorganisms have substantially hindered the sustainable growth of the prawn's farming industry. Nutritional regulation is considered a key strategy for improving disease resistance in aquatic animals.
Aims: A feeding trial was conducted to evaluate the effects of dietary astaxanthin on growth performance, intestinal microbiota and metabolites, antioxidant capacity, immune response, and resistance to decapod iridescent virus 1 (DIV1) in Macrobrachium rosenbergii.
Environ Microbiol Rep
October 2025
Department of Biology, Marine Biology Section, University of Copenhagen, Helsingør, Denmark.
Due to climate change, sea ice more commonly retreats over the shelf breaks in the Arctic Ocean, impacting sea ice-pelagic-benthic coupling in the deeper basins. Nitrogen fixation (the reduction of dinitrogen gas to bioavailable ammonia by microorganisms called diazotrophs) is reported from Arctic shelf sediments but is unknown from the Arctic deep sea. We sampled five locations of deep-sea (900-1500 m) surface sediments in the central ice-covered Arctic Ocean to measure potential nitrogen fixation through long-term (> 280 days) stable-isotope (N) incubations and to study diazotroph community composition through amplicon sequencing of the functional marker gene nifH.
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