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A single particle nanofabrication technique was successfully applied to the fabrication of homogeneous poly(N-isopropylacrylamide) (PNIPAAm) 1D nanogels over a large area, using N,N'-methylene-bis-acrylamide (MBAAm) as a cross-linker. The PNIPAAm 1D nanogels with high aspect ratio over 130 were formed uniformly on the substrate, and the mechanical strength and the length of the 1D nanogels can be easily controlled by adjusting the MBAAm content. The 1D nanogels were transformed from the non-aggregated to aggregated forms over a lower critical solution temperature (LCST) of approximately 32 °C in water. Precise trace of the temperature induced change in the size of the 1D nanogel was well interpreted by the coil-to-globule transition of PNIPAAm, which was clearly visualized in the present study. This is the first report of uniform shape change for a 1D nanogel by external stimulus over a large area.
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http://dx.doi.org/10.1021/am3014086 | DOI Listing |
Acta Crystallogr D Struct Biol
October 2025
Turkish Accelerator and Radiation Laboratory, 06830 Ankara, Türkiye.
Membrane-protein quality control in Escherichia coli involves coordinated actions of the AAA+ protease FtsH, the insertase YidC and the regulatory complex HflKC. These systems maintain proteostasis by facilitating membrane-protein insertion, folding and degradation. To gain structural insights into a putative complex formed by FtsH and YidC, we performed single-particle cryogenic electron microscopy on detergent-solubilized membrane samples, from which FtsH and YidC were purified using Ni-NTA affinity and size-exclusion chromatography.
View Article and Find Full Text PDFJ Appl Clin Med Phys
September 2025
Department of Radiation Oncology, Virginia Commonwealth University, Richmond, Virginia, USA.
Purpose: Real‑time magnetic resonance-guided radiation therapy (MRgRT) integrates MRI with a linear accelerator (Linac) for gating and adaptive radiotherapy, which requires robust image‑quality assurance over a large field of view (FOV). Specialized phantoms capable of accommodating this extensive FOV are therefore essential. This study compares the performance of four commercial MRI phantoms on a 0.
View Article and Find Full Text PDFJ Immunol
September 2025
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Qidong-Fudan Innovative Institution of Medical Sciences, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Hepatitis B virus (HBV) exclusively infects hepatocytes and produces large quantities of subviral particles containing its surface antigen (HBsAg). T cells play a central role in controlling HBV infection but can also mediate liver injury and contribute to disease progression. However, the mechanisms that regulate T-cell responses to eliminate the virus without causing immunopathology during acute HBV infection remain poorly defined.
View Article and Find Full Text PDFJ R Soc Interface
September 2025
Institute of Intelligent Systems and Robotics, Sorbonne Université, Paris, Île-de-France, France.
A number of techniques have been developed to measure the three-dimensional trajectories of protists, which require special experimental set-ups, such as a pair of orthogonal cameras. On the other hand, machine learning techniques have been used to estimate the vertical position of spherical particles from the defocus pattern, but they require the acquisition of a labelled dataset with finely spaced vertical positions. Here, we describe a simple way to make a dataset of images labelled with vertical position from a single 5 min movie, based on a tilted slide set-up.
View Article and Find Full Text PDFJ Colloid Interface Sci
September 2025
Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650093, China.
Encapsulation of non-noble bimetallic nanoparticles within a zeolite framework can improve the stability and accessibility of active sites, but the single microporous structure and poor metal stability decreased the catalytic performance of the catalyst. Here, 3D hierarchical ZSM-5 zeolite encapsulated NiCo nanoparticles (NiCo@3DHZ5) were synthesized by Bottom-up confined steam-assisted crystallization (SAC) one-pot hydrothermal method and applied to the hydrodeoxygenation of vanillin. A series of characterizations showed that highly stable alloyed NiCo nanoparticles were encapsulated in a framework of 3DHZ5, the strong metal-zeolite interactions resulted in highly dispersed NiCo nano-alloys facilitated hydrogen adsorption and spillover of active hydrogen atoms, and the 3D hierarchical structure promoted oxygenated substrate diffusion, the synergy interaction between the alloy particles confined in the 3DHZ5 pores and the acidic sites on the zeolite surface promoted the selective conversion of vanillin.
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