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A typical marine bacterial cell in coastal seawater contains only ∼200 molecules of mRNA, each of which lasts only a few minutes before being degraded. Such a surprisingly small and dynamic cellular mRNA reservoir has important implications for understanding the bacterium's responses to environmental signals, as well as for our ability to measure those responses. In this perspective, we review the available data on transcript dynamics in environmental bacteria, and then consider the consequences of a small and transient mRNA inventory for functional metagenomic studies of microbial communities.
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http://dx.doi.org/10.1038/ismej.2012.94 | DOI Listing |
Microb Pathog
August 2025
Department of Biological and Environmental Sciences, Qatar University, Doha, Qatar.
Coral growth anomalies (GA) affect many coral genera across the world, yet the etiology of GAs remains unknown, with limited knowledge of associated bacteria. In this study, we investigated bacterial associations between the growth anomalies (GAs) and healthy (H) portions of coral colonies in Acropora faraonis for two seasons to understand microbial dynamics. Additionally, we examined bacteria in water (W), which could be affecting coral bacterial communities.
View Article and Find Full Text PDFEnviron Sci Ecotechnol
May 2025
Department of Civil, Structural & Environmental Engineering, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland.
Microbial aggregates of different sizes in aerobic granular sludge (AGS) systems have been shown to exhibit distinct microbial community compositions. However, studies comparing the microbial activities of different-sized aggregates in AGS systems remain limited. In this study, genome-resolved metatranscriptomics was used to investigate microbial activity patterns within differently sized aggregates in a full-scale AGS plant.
View Article and Find Full Text PDFISME J
January 2025
Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, United States.
SAR202 bacteria are a diverse group of bacteria in the ocean. The SAR202 lineages dominate the bacterial community and evolve specialized metabolisms for oxidizing recalcitrant organic compounds in the dark ocean. SAR202 bacteria are also present in the euphotic oceans; however, their ecological roles and metabolic potential remain poorly understood.
View Article and Find Full Text PDFViruses
March 2025
Institute for Biosecurity and Microbial Forensics, Oklahoma State University, Stillwater, OK 74078, USA.
is a recently accepted plant virus genus within the family . Species assigned to the genus consist of bi-segmented, negative sense, single-stranded RNA viruses and are transmitted by spp. Currently, there are five recognized species and two unclassified members in the genus Four out of seven-orchid fleck virus (OFV), citrus leprosis virus N, citrus chlorotic spot virus, and citrus bright spot virus-can infect citrus and produce leprosis disease-like symptoms.
View Article and Find Full Text PDFEnviron Sci Technol
April 2025
Department of Civil, Structural & Environmental Engineering, Trinity College Dublin, The University of Dublin, Dublin D2, Ireland.
Understanding the relative influence of immigration and species sorting in wastewater treatment systems is essential, as bacteria in influent wastewater can significantly impact treatment system functionality. This study investigated the contribution of immigration to the community assembly of different-sized microbial aggregates in a full-scale aerobic granular sludge (AGS) system using genome-resolved metatranscriptomics. Our novel analysis revealed that negative-net-growth-rate populations, which persist due to immigration, can exhibit substantial activity and potentially contribute to the AGS system's functionality.
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