Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The effect of ionization modifiers added to the mobile phase of nanoflow liquid chromatography-tandem mass spectrometry (nLC-ESI-MS³) on the simultaneous analysis of all phospholipid (PL) classes in negative ion mode has been investigated. While MS analysis of most PL classes is carried out in negative ion mode, analysis of neutral polar (polar but electrically neutral) lipids like phosphatidylcholine (PC) and sphingomyelin (SM) is highly efficient in positive ion mode. Therefore, analysis of PL mixture samples often requires two separate runs in both positive and negative ion mode. In order to establish run conditions to carry out a single nLC-ESI-MS-MS for all PLs, the ionization efficiency of 13 different types of PL molecules in nLC-ESI-MS has been evaluated in negative ion mode by varying the modifiers and their concentrations. Experiments demonstrated that a mixture of 0.05% ammonium hydroxide and 1 mM ammonium formate added to the mobile phase provided effective ionization for all classes of PLs. The optimized conditions were applied to the analysis of a phospholipid mixture extracted from a human urine sample, yielding the identification of a total of 85 PL species. Analysis of the same sample with dual nLC-ESI-MS² runs in both positive and negative ion mode confirmed that nLC-ESI-MS³ with the mixed modifier run only in negative ion mode gave comparable results.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.chroma.2012.03.073 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Isolation and characterization of β-sitosterol from Terminalia arjuna fruit and its growth-promoting effect on beneficial fungi.
Cell Mol Biol (Noisy-le-grand)
September 2025
IQRAA Centre for Research and Development, IQRAA International Hospital and Research Centre, Kozhikode, Kerala, India.
Terminalia arjuna, an important medicinal plant in traditional Indian systems, has been extensively studied for its cardioprotective bark. However, limited attention has been given to its fruit, which contains several biologically active phytochemicals with potential antioxidant, anti-inflammatory, and immunomodulatory properties. This study aimed to isolate and partially purify phytoactive compounds from the fruit of T.
View Article and Find Full Text PDFJanus MXene Fiber Constructed via Flake Orientation Engineering.
Adv Mater
September 2025
Center for Renewable Energy and Storage Technologies (CREST), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.
The orientation of MXene flakes has received increasing research attention as it plays a critical role in determining the performance of MXene-based assemblies. Engineering MXene flakes into horizontal or vertical orientations can offer distinct advantages such as higher electrical conductivity, higher mechanical strength, and more efficient ion/molecule transport across the flakes. However, the benefits of horizontal and vertical orientations are mutually exclusive, and both of them possess structural symmetry that restricts their ability for stimuli-responsive deformation.
View Article and Find Full Text PDFUntargeted metabolomics reveals the metabolic characteristics and biomarkers of obstetric antiphospholipid syndrome and undifferentiated connective tissue disease.
Front Mol Biosci
August 2025
Department of Rheumatology and Immunology, Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Mianyang, Sichuan, China.
Background: The clinical differentiation between obstetric antiphospholipid syndrome (OAPS) and undifferentiated connective tissue disease (UCTD) presents significant diagnostic challenges. This study employs metabolomics to investigate metabolic reprogramming patterns in OAPS and UCTD, aiming to identify potential biomarkers for early diagnosis.
Methods: Using LC-MS-based metabolomics, we analyzed serum profiles from 40 OAPS patients (B1), 30 OAPS + UCTD patients (B2), 27 UCTD patients (B3), and 30 healthy controls (A1).
Dehydrocostus Lactone Effectively Alleviates Inflammatory Diseases by Covalently and Irreversibly Targeting NLRP3.
MedComm (2020)
September 2025
The activation of nucleotide oligomerization domain-like receptor (NLR) family, pyrin domain-containing protein 3 (NLRP3) inflammasome is implicated in the pathogenesis of various inflammatory diseases. The natural product oridonin possesses a novel mechanism for NLRP3 inhibition and a unique binding mode with NLRP3, but its poor anti-inflammatory activity limits further application. After virtual screening of diverse natural product libraries, dehydrocostus lactone (DCL) was considered as a potential NLRP3 inhibitor.
View Article and Find Full Text PDFmonitoring of polarons in a mixed conducting polymer using ultrafast transient absorption spectroelectrochemistry.
Chem Sci
August 2025
Department of Chemistry and Biochemistry, Auburn University Auburn Alabama 36849 USA
Organic mixed ionic-electronic conducting polymers remain at the forefront of materials development for bioelectronic device applications. During electrochemical operation, structural dynamics and variations in electrostatic interactions in the polymer occur, which affect dual transport of the ions and electronic charge carriers. Such effects remain unclear due to a lack of spectroscopic methods capable of capturing these dynamics, which hinders the rational design of higher-performance polymers.
View Article and Find Full Text PDF