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The automated assessment of the sperm quality is an important challenge in the veterinary field. In this paper, we explore how to describe the acrosomes of boar spermatozoa using image analysis so that they can be automatically categorized as intact or damaged. Our proposal aims at characterizing the acrosomes by means of texture features. The texture is described using first order statistics and features derived from the co-occurrence matrix of the image, both computed from the original image and from the coefficients yielded by the Discrete Wavelet Transform. Texture descriptors are evaluated and compared with moments-based descriptors in terms of the classification accuracy they provide. Experimental results with a Multilayer Perceptron and the k-Nearest Neighbours classifiers show that texture descriptors outperform moment-based descriptors, reaching an accuracy of 94.93%, which makes this approach very attractive for the veterinarian community.
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http://dx.doi.org/10.1016/j.cmpb.2012.01.004 | DOI Listing |
J Anim Sci Biotechnol
September 2025
Department of Biotechnology, CRESCO, Centre for Embryology and Healthy Development, University of Inland Norway, Holsetgata 31, 2318, Hamar, Norway.
Background: Boars undergo physiological and biochemical changes in semen composition as they grow from puberty to sexual maturity. However, comprehensive metabolomic profiles of boar semen remain uncharacterised. Understanding metabolic alterations in semen during this period is important for optimising reproductive performance in breeding programs.
View Article and Find Full Text PDFCommun Biol
August 2025
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Succinylation is a recently identified post-translational modification (PTM) that modulates enzyme activity and metabolism. However, how it regulates metabolic reprogramming in sperm remains unclear. In this study, we show that succinylation of pyruvate kinase M2 (PKM2) promotes oxidative phosphorylation (OXPHOS) and linear motility in boar sperm.
View Article and Find Full Text PDFReprod Toxicol
August 2025
Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Republic of Korea; Research Institute for Innovative Animal Science, Kyungpook National University, Sangju 37224, Republic of Korea. Electronic address:
The potential toxicity of abamectin (ABM), a macrocyclic lactone insecticide widely used in agriculture, in non-target organisms has raised concerns. Therefore, this study aimed to investigate the effects of ABM on sperm function, focusing on motility, kinematics, ATP levels, viability, protein kinase A (PKA) activity, and tyrosine phosphorylation. Boar spermatozoa were incubated with various ABM concentrations (0, 1, 5, 10, 15, 25, 50, and 100 µM) under capacitating conditions and assessed through computer-assisted sperm analysis, Hoechst 33258/chlortetracycline staining, ATP and viability assays, and Western blotting.
View Article and Find Full Text PDFAnim Sci J
August 2025
Bio-Innovation Research Center, Tokushima University, Tokushima, Japan.
As zinc is important for the fertilization competency of sperms and oocytes, we investigated the effects of zinc chloride (ZnCl) supplementation during in vitro fertilization (IVF) on porcine oocyte fertilization and development. We evaluated the effects of ZnCl concentration (0, 1, 10, and 20 μg/mL) on the quality of frozen-thawed boar spermatozoa cultured for 5 h and on oocyte fertilization and embryo development after IVF. Spermatozoa from three different boars were additionally tested.
View Article and Find Full Text PDFReprod Domest Anim
August 2025
College of Life Sciences and Agri-forestry, Southwest University of Science and Technology, Mianyang, Sichuan, China.
Semen quality plays a critical role in artificial insemination; however, the accumulation of oxidised substances during semen storage impairs sperm viability and function, ultimately reducing fertility. Sodium phytate is a naturally occurring compound found in plants, known for its potent antioxidant properties. The aim of this study was to evaluate the effect of sodium phytate on the preservation of porcine semen at various storage temperatures (17°C and 4°C).
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