Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Anterior gradient-2 (AGR2) promotes tumor growth, cell migration and cellular transformation and its enhanced expression is almost completely restricted to malignant tissues, thus making AGR2 an interesting target for the development of immunotherapeutic strategies. We investigated whether the AGR2 molecule comprises human leukocyte antigen (HLA)-A*0201-binding epitopes recognized by human cytotoxic T lymphocytes (CTLs), which could be targeted in dendritic cell (DC)-based cancer immunotherapy against colorectal cancer (CRC). We reviewed the sequence of AGR2 for peptides that could potentially bind to HLA-A*0201 with the aid of a computer-based program. Five candidate peptides with different binding scores were synthesized and tested. These peptides were then assessed for their immunogenicity to elicit specific immune responses mediated by CTLs in vitro by means of enzyme-linked immunospot assays and CTL assays. AGR2 was highly expressed in several CRC cell lines, including DK01, DLD1, KM12C, HCT-8 and HT-29. DCs pulsed with AGR2-P2 (aa 11-19; LLVALSYTL) or AGR2-P4 (aa 127-135; RIMFVDPSL) generated potent CTLs that could lyse T2 cells pulsed with AGR2-P2 or AGR2-P4 and HLA-A0201(+) AGR2-positive CRC cell lines in a strong dose-dependent and HLA-A*0201-restricted manner. In conclusion, these novel epitopes derived from AGR2 protein may be attractive candidates for DC-based immunotherapy for CRC.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002806 | PMC |
| http://dx.doi.org/10.1038/cmi.2011.52 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Using MUC2 mucin producing tumorigenic human goblet-like cells to uncover functional properties of the mucus barrier.
Gut Microbes
December 2025
Departments of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada.
Goblet cells are the guardians that produce MUC2 mucin that forms the protective mucus barrier as the first line of innate host defense against pathogen invasion while sustaining a healthy gut microbiota. Using an unbiased screen, we cloned a high LS174T colonic adenocarcinoma MUC2 mucin-producing goblet-like cell that revealed by whole genome sequencing, mutations that upregulated the expression of tumor suppressor , anterior gradient 2 () and mitogen-activated protein kinase kinases ( and ) genes that enhanced MUC2 biosynthesis and secretion. Hypersecretory () cells exhibited a phenotype characterized by high constitutive mRNA, upregulation of glycosyltransferases, and hypersecretion of sialylated and fucosylated MUC2 mucus enumerated by glycomics analysis.
View Article and Find Full Text PDFFlavoxanthin Binds to AGR2 to Mediate Fatty Acid Oxidation and Reinforce Anoikis in Lung Adenocarcinoma.
Drug Dev Res
August 2025
Interventional Department, Jinhua Hospital affiliated to Wenzhou Medical University, Jinhua, China.
Traditional Chinese medicine (TCM) can not only alleviate symptoms in cancer patients and improve their life quality but also serve as an adjuvant therapy to reduce the toxic side effects of chemotherapy and radiotherapy, making it a hot topic in anti-tumor drug development in recent years. This project was designed to probe into the small molecular Chinese medicine components with targeted effects on lung adenocarcinoma (LUAD) and further reveal their mechanisms. Based on The Cancer Genome Atlas dataset analysis of anterior gradient-2 (AGR2) expression in LUAD, Autodock docking and scoring were employed to screen small molecular drugs that bound to AGR2.
View Article and Find Full Text PDFBackground: Neuroimaging studies of functional neurological disorder (FND), a condition at the intersection of psychiatry and neurology, often rely on discrete connections or parcellations that may obscure the brain's functional network architecture. This study applied a gradient-based approach to examine macroscale cortical organization in FND.
Methods: We analyzed resting-state functional magnetic resonance imaging (fMRI) data from 64 patients with mixed FND (FND-mixed), 61 age- and sex-matched healthy controls (HCs), and 62 psychiatric controls (PCs) matched on age, sex, depression, anxiety, and post-traumatic stress disorder (PTSD) severity.
Agr2 in cancer and beyond: unraveling its role during protein synthesis, ER stress, and as a predictive biomarker.
Mol Cell Biochem
June 2025
Department of Biological Sciences, North Dakota State University, Fargo, ND, USA.
Protein folding is an essential component of protein biosynthesis, allowing for post-translational modifications that ensure proper protein structure and function to support cellular physiology. The presence of unfolded proteins triggers cellular mechanisms to either remove the unfolded proteins or reduce protein synthesis. However, the accumulation of improperly folded proteins may lead to diseases, including neurological disorders and cancers.
View Article and Find Full Text PDFHigh-Throughput Drug Screening of Clear Cell Ovarian Cancer Organoids Reveals Vulnerability to Proteasome Inhibitors and Dinaciclib and Identifies AGR2 as a Therapeutic Target.
Cancer Res Commun
June 2025
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
Unlabelled: There are currently no effective treatments available for clear cell ovarian cancer (CCC). In this study, we aimed to identify effective drugs for CCC through high-throughput drug screening (HTDS) using ovarian cancer organoids and determine novel therapeutic targets based on the biological characteristics of CCC through omics analysis. An ovarian cancer organoid biobank was established, and HTDS was conducted using CCC organoids based on libraries of 361 and 4,560 compounds.
View Article and Find Full Text PDF