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http://dx.doi.org/10.2323/jgam.57.169 | DOI Listing |
Biotechnol Rep (Amst)
September 2025
Technical University of Munich, Germany, TUM Campus Straubing for Biotechnology and Sustainability, Bioprocess Engineering, Uferstraße 53, D-94315 Straubing, Germany.
Exopolysaccharides are biopolymers with wide-ranging industrial applications. To substitute fossil-based by bio-based, biodegradable polymers, exopolysaccharide production needs to become much more efficient. Pullulan, produced by , is popular for its unique properties like film-formation, adhesiveness, biodegradability, etc.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life
The low yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) restricts their industrial application. To overcome this limitation, a single-factor optimization strategy was applied to develop co-culture system involving Weissella confusa P2 and Candida shehatae. This approach resulted in 48.
View Article and Find Full Text PDFBiosci Biotechnol Biochem
September 2025
Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan.
Glycoside hydrolase family 27 (GH27) consists primarily of exo-acting enzymes, including α-galactosidase, α-N-acetylgalactosaminidase, β-L-arabinopyranosidase, and isomalto-dextranase. Here, we examined the gene expression profiles and biochemical characteristics of a multi-domain protein, Fjoh_4436 (named FjGH27A), which contains a GH27 catalytic domain and is encoded upstream of the previously described branched dextran utilization locus in Flavobacterium johnsoniae. Expression of this gene increased 7.
View Article and Find Full Text PDFBiomed Pharmacother
September 2025
APC Microbiome Ireland, University College Cork, T12 YT20 Cork, Ireland; Cancer Research @UCC, College of Medicine and Health, University College Cork, T12 K8AF Cork, Ireland.
Microbial exopolysaccharides (EPS) are emerging as critical effector molecules orchestrating host immunity. Their remarkable structural diversity, driven by variations in monosaccharide composition, charge, and glycosidic linkages, dictates potent immunomodulatory functions through specific interactions with host pattern recognition receptors (CLRs, TLRs), triggering defined cellular responses and cytokine profiles, often with minimal toxicity. Beyond direct immune effects, EPS shape the gut environment by fortifying barrier integrity and acting as prebiotics.
View Article and Find Full Text PDFData Brief
October 2025
Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham, 44150, Thailand.
This study aimed to evaluate the optimal nitrogen source for the production of antioxidant exopolysaccharides (EPSs) by KW8 using cassava pulp carbon source. Various nitrogen sources were initially screened, and yeast extract was identified as the most effective. Further optimization was conducted using Response Surface Methodology (RSM) with a Central Composite Design (CCD) to assess the influence of key parameters, including pH, inoculum size, carbon-to-nitrogen (C/N) ratio, and agitation speed on EPS production.
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