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The coimmobilization of nitrobenzene nitroreductase and glucose-6-phosphate dehydrogenase in silica particles enables the continuous conversion of nitrobenzene to hydroxylaminobenzene with NADPH recycling.
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http://dx.doi.org/10.1039/b604689d | DOI Listing |
Cell Rep Phys Sci
July 2025
Center for Cooperative Research in Biomaterials (CIC biomaGUNE) - Basque Research and Technology Alliance (BRTA), Paseo Miramón, 194, 20014 Donostia-San Sebastián, Spain.
Self-sufficient heterogeneous biocatalysts (ssHBs), in which enzymes and cofactors are coimmobilized on the same support, provide cofactor regeneration and reduce operating costs. However, the underlying mechanisms remain poorly understood. Here, we present a theoretical model for ssHBs consisting of NAD(P)H-dependent dehydrogenases immobilized on porous agarose-based materials with cofactors coimmobilized through electrostatic interactions via a cationic polymer coating.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
August 2025
Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Global energy and environmental crises have stimulated increased efforts toward converting CO into valuable chemicals or energy substance. Inspired by natural chloroplasts and mitochondria, we build an innovative polydopamine-armored multiple enzyme microreactor for co-immobilizing glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 3-phosphoglyceric phosphokinase (PGK), formate dehydrogenase (FDH), and ATPase-incorporating proteoliposome, providing spatially confined microenvironments akin to natural systems. Within this microreactor, GAPDH and PGK catalyze the conversion of glyceraldehyde 3-phosphate to 3-phosphoglyceric acid, reducing β-nicotinamide adenine dinucleotide (NAD) to NADH and generating a proton influx that drives ATP synthesis.
View Article and Find Full Text PDFEnzyme Microb Technol
September 2025
Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science and Engineering, Hubei University, Wuhan 430062, China. Electronic address:
The crosslinked enzyme aggregate (CLEA) technique has been developed as an easy and convenient strategy for carrier-free immobilization of enzymes. However, the irregular voids of enzyme aggregates limit the controlled crosslinking process by using regular crosslinkers such as glutaraldehyde. To overcome this limitation, here we have developed a simple strategy for the preparation of hyperbranched polymer-crosslinked laccase aggregates (HPCLEAs).
View Article and Find Full Text PDFInt J Biol Macromol
June 2025
School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, PR China; School of Chemical Engineering and Technology, State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300350, PR China; Tianjin Key Laboratory of Membrane Science and Desalination Technolog
The enzymatic oxidation of amino acids using amino acid oxidases (AAOs) as biocatalysts for α-keto acid synthesis has garnered increasing attention due to its environmental friendliness and exceptional reaction efficiency. However, the by-product HO inevitably causes the decarboxylation of α-keto acids and subsequently deactivates the enzyme. Herein, we used a simple in-situ biomineralization method to immobilize Pt NPs with catalase-like activity and L-amino acid oxidase (LAAO) in to a hydrogen-bonded organic framework (HOF) under a mild condition, and the synthesized LAAO-Pt@HOF cascade catalytic system was applied to synthesis of α-keto acid.
View Article and Find Full Text PDFBiosensors (Basel)
April 2025
Department of Chemical Engineering, Ariel University, Kyriat-ha-Mada, Ariel 4070000, Israel.
Amperometric biosensors (ABSs) and enzymatic biofuel cells (BFCs) share several fundamental principles in their functionality, despite serving different primary purposes. Both devices rely on biorecognition, redox reactions, electron transfer (ET), and advanced electrode materials, including innovative nanomaterials (NMs). ABSs and BFCs, utilizing microbial oxidoreductases in combination with electroactive NMs, are both efficient and cost-effective.
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