Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure is similar to that of Sso10b1 (also called Alba) from the same organism. However, Sso10b2 contains an arginine-rich loop RDRRR motif, which may play an important role in nucleic acid binding. There are two independent Sso10b2 proteins in the asymmetric unit, and a plausible stable dimer could be deduced from the crystal structure. Topology comparison revealed that Sso10b2 is similar to several RNA-binding proteins, including IF3-C, YhhP, and DNase I. Models of the Sso10b2 dimer bound to either B-DNA or A-DNA have been constructed.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC164892 | PMC |
| http://dx.doi.org/10.1128/JB.185.14.4066-4073.2003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structural Elucidation and Covalent Modulation of the Autorepressed Orphan Nuclear Receptor NR2F6.
ACS Chem Biol
September 2025
Laboratory of Chemical Biology, Department of Biomedical Engineering and Institute of Complex Molecular Systems, Technische Universiteit Eindhoven, 5612 AZ Eindhoven, The Netherlands.
The orphan nuclear receptor NR2F6 (Nuclear Receptor subfamily 2 group F member 6) is an emerging therapeutic target for cancer immunotherapy. Upregulation of NR2F6 expression in tumor cells has been linked to proliferation and metastasis, while in immune cells NR2F6 inhibits antitumor T-cell responses. Small molecule modulation of NR2F6 activity might therefore be a novel strategy in cancer treatment, benefiting from this dual role of NR2F6.
View Article and Find Full Text PDFCombination of Microcrystal Electron Diffraction and Solid-State NMR for the Structural Determination of Indomethacin-Nicotinamide Co-crystals.
Chem Pharm Bull (Tokyo)
September 2025
National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan.
The development of analytical techniques applicable to powdered pharmaceutical co-crystals, including those containing excipients, represents a comprehensive strategy for quality control in both drug development and regulatory settings. This study investigates the structural characterization of indomethacin-nicotinamide co-crystals using a combination of microcrystal electron diffraction (microED), solid-state NMR (SSNMR), Raman spectroscopy, and powder X-ray diffraction (PXRD). MicroED analysis revealed the crystal structure of the co-crystal, while SSNMR measurements provided insights into the molecular interactions within the structure.
View Article and Find Full Text PDFCrystal structures of distinct parallel and antiparallel DNA G-quadruplexes reveal structural polymorphism in C9orf72 G4C2 repeats.
Nucleic Acids Res
September 2025
State Key Laboratory of Vaccines for Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361102, Fujian, China.
The abnormal expansion of GGGGCC (G4C2) repeats in the noncoding region of the C9orf72 gene is a major genetic cause of two devastating neurodegenerative disorders, amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). These G4C2 repeats are known to form G-quadruplex (G4) structures, which are hypothesized to contribute to disease pathogenesis. Here, we demonstrated that four DNA G4C2 repeats can fold into two structurally distinct G4 conformations: a parallel and an antiparallel topology.
View Article and Find Full Text PDFImpact of various reaction conditions of solvothermal synthesis on electrochemical properties of Ni-MOF materials.
Nanotechnology
September 2025
Shanghai Polytechnic University, No. 2360 Jinhai Road, Shanghai 201209, P.R. China, Shanghai, Shanghai, 201209, CHINA.
A series of Ni-MOF materials were synthesized by a one-step solvothermal method under different reaction conditions, including metal source, organic ligand, reaction time and reaction temperature. The results demonstrated that the Ni-MOFs synthesized with Ni(NO3)2•6H2O as the metal source had higher crystallinity and a more uniform crystalline structure than those with NiCl2•6H2O. Different organic ligands led to the formation of Ni-MOFs in various morphologies.
View Article and Find Full Text PDFImprovement of oat β-glucan on the quality deterioration of frozen wheat dough and steamed bread.
Food Chem
September 2025
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, China; National Engineering Rese
Investigating how cereal-derived polysaccharides mitigate the cold denaturation of dough carries substantial significance for improving the quality of cereal-based products. This study aimed to assess the effects of laboratory-prepared oat β-glucan and commercial oat β-glucan on the structural properties and quality of wheat flour, frozen dough, and the resultant steamed bread. Our data indicated that β-glucan with higher molecular weight (1.
View Article and Find Full Text PDF