Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Molecular beacons (MBs) are oligonucleotide probes having a compact hairpin structure, with a fluorophore attached to one end and a quencher molecule attached to the other end. In its native state, the fluorophore is quenched by virtue of its proximity to the quencher molecule. Upon hybridization with its complementary oligonucleotide target, fluorescence is elicited due to a conformational change that results in separation of the fluorophore and quencher molecule. The present study describes the hybridization interaction of an MB to various complementary target sequences. The effects of temperature and length of complementary target sequences on hybridization were investigated using capillary electrophoresis and solution-based fluorescence techniques. Hybridization efficiency was dependent on the ability of the target sequences to destabilize the stem region by binding directly to the stem region. Optimal hybridization occurred between 40 and 50 degrees C for all targets tested, with the true target forming a more stable hybrid complex.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/elps.200390033 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sensitive Bioassay with an Ultralarge Dynamic Range via Microlaser Ensemble Quenching.
ACS Sens
September 2025
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States.
We present a bioassay platform that leverages the lasing threshold distribution in a microlaser ensemble (ME), consisting of hundreds of individual microlasers, to measure analyte concentrations in solution. An ME is formed by placing dye-doped microbeads in a micro Fabry-Perot cavity. The microbeads are surface-modified with biorecognition molecules to capture analytes, while the quenchers resulting from the presence of the analytes on the microbeads' surfaces increase the lasing thresholds of the microlasers.
View Article and Find Full Text PDFHigh-throughput competitive binding assay for targeting RNA tertiary structures with small molecules: application to pseudoknots and G-quadruplexes.
Nucleic Acids Res
August 2025
Department of Medical Biochemistry, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
There is an indisputable need for new screening methodologies to identify small molecules that target RNA tertiary structures, such as pseudoknots or G-quadruplexes. Here, we present a high-throughput competitive binding antisense assay designed to identify ligands for complex RNA tertiary structures. In this assay, initially customized for the bacterial PreQ1-I riboswitch pseudoknot, ligands compete with a rationally designed quencher-labelled antisense oligonucleotide for binding to a fluorophore-labelled riboswitch.
View Article and Find Full Text PDFFRET Materials for Biosensing and Bioimaging.
Chem Rev
August 2025
Department of Engineering Physics, McMaster University, Hamilton, Ontario M8S 4K1, Canada.
Förster resonance energy transfer (FRET) spectroscopy and microscopy are constantly expanding sensing techniques for analyzing biomolecular interactions. In addition to the biological recognition molecules and biological or chemical analytes, the most important components for designing FRET biosensing systems are the materials that constitute the FRET donor-acceptor pair. These FRET materials consist of small molecules, biological or chemical nanoscaffolds, or nanomaterials that function in the ultraviolet, visible, or infrared spectral range.
View Article and Find Full Text PDFDNA Origami Tension Sensors (DOTS) for Single-Molecule Force Measurements at Fluid Intermembrane Junctions.
Nano Lett
September 2025
Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322, United States.
A key event in triggering adaptive immunity is the binding of a T cell receptor (TCR) to its antigen at the T cell-target cell interface. Mechanical forces are critical for TCR-antigen interactions, where piconewton (pN) forces modulate immune responses. A major challenge in studying these interactions is quantifying forces at the single-molecule scale, as T cells can activate in response to just 1-10 antigen molecules.
View Article and Find Full Text PDFFlexTORCH: An Improved Flexible Fluorophore-Linker-Quencher Molecule Enlightening ADC Research.
Bioconjug Chem
August 2025
Department of Discovery Technologies, The Healthcare Business of Merck KGaA, 64293 Darmstadt, Germany.
Antibody-drug conjugates (ADCs) are an emerging class of molecules for cancer therapy. An ADC consists of an antibody that is attached to a toxic payload via a linker molecule. Once the ADC is internalized into the cancer cell, the payload is released inside the cell, which leads to tumor cell death.
View Article and Find Full Text PDF