Identification of monocyte-associated genes MSRB2, CLEC4D, and ASGR2 as potential biomarkers for tuberculosis via machine learning and mendelian randomization.

Objective: This study explores the link between immune cells and tuberculosis (TB) pathogenesis and progression, proposing diagnostic strategies based on immune microenvironment changes.

Methods: The CIBERSORT algorithm assessed immune cell infiltration in TB tissues, validated by routine blood tests. Differential expression analysis and WGCNA identified key genes and modules.

Exploring T-cell metabolism in tuberculosis: development of a diagnostic model using metabolic genes.

Objectives: The early diagnosis and immunoregulatory mechanisms of active tuberculosis (ATB) and latent tuberculosis infection (LTBI) remain unclear, and the role of metabolic genes in host-pathogen interactions requires further investigation.

Methods: Single-cell RNA sequencing (scRNA-seq) was applied to analyze peripheral blood mononuclear cells (PBMCs) from 7 individuals, including 2 healthy controls (HC), 2 LTBI patients, and 3 ATB patients. We identified T-cell-associated metabolic differentially expressed genes (TCM-DEGs) through integrated differential expression analysis and machine learning algorithms (XGBoost, SVM-RFE, and Boruta).

A new species of the genus Logunov, 1999 from Wuyi Mountain National Park, China (Araneae, Salticidae).

Background: The genus Logunov, 1999, a group of jumping spiders (Salticidae), has been recorded across multiple regions in China. Current records indicate its presence in Anhui, Hunan, Yunnan, Hubei, Taiwan, Guangdong, Hong Kong, Guizhou, Fujian, Chongqing and Sichuan Provinces. These reports highlight a fragmented, but widespread distribution in southern and central China, particularly in mountainous or subtropical habitats.

Diversity of jumping spiders (Araneae, Salticidae) in the Jinggangshan National Nature Reserve, Jiangxi, China.

A list of 94 salticid species of 48 genera collected from Jinggangshan National Nature Reserve in Jiangxi Province is provided. Four new salticid species are diagnosed, described, and illustrated: K. Liu, , K.

Identification of diagnostic biomarkers and molecular subtype analysis associated with m6A in Tuberculosis immunopathology using machine learning.

Tuberculosis (TB), ranking just below COVID-19 in global mortality, is a highly complex infectious disease involving intricate immunological molecules, diverse signaling pathways, and multifaceted immune processes. N6-methyladenosine (m6A), a critical epigenetic modification, regulates various immune-metabolic and pathological pathways, though its precise role in TB pathogenesis remains largely unexplored. This study aims to identify m6A-associated genes implicated in TB, elucidate their mechanistic contributions, and evaluate their potential as diagnostic biomarkers and tools for molecular subtyping.

Combining bioinformatics and machine learning to identify diagnostic biomarkers of TB associated with immune cell infiltration.

Objective: The asymptomatic nature of tuberculosis (TB) during its latent phase, combined with limitations in current diagnostic methods, makes accurate diagnosis challenging. This study aims to identify TB diagnostic biomarkers by integrating gene expression screening with machine learning, evaluating their diagnostic potential and correlation with immune cell infiltration.

Methods: We analyzed GSE19435, GSE19444, and GSE54992 datasets to identify differentially expressed genes (DEGs).

Prognostic risk model of LIHC T-cells based on scRNA-seq and RNA-seq and the regulation of the tumor immune microenvironment.

Background: T-cell-related genes play a crucial role in LIHC development. However, a reliable prognostic profile based on risk models of these genes has yet to be identified.

Methods: Single-cell datasets from both tumor and normal tissue samples were obtained from the GEO database.

Interleukin-17F suppressed colon cancer by enhancing caspase 4 mediated pyroptosis of endothelial cells.

The combination of anti-angiogenic treatment and immunotherapy presents a promising strategy against colon cancer. Interleukin-17F (IL-17F) emerges as a critical immune cell cytokine expressed in colonic epithelial cells, demonstrating potential in inhibiting angiogenesis. In order to clarify the roles of IL-17F in the colon cancer microenvironment and elucidate its mechanism, we established a mouse colon carcinoma cell line CT26 overexpressing IL-17F and transplanted it subcutaneously into syngeneic BALB/c mice.

Effect and Mechanism of Mycobacterium avium MAV-5183 on Apoptosis of Mouse Ana-1 Macrophages.

To investigate the effects and mechanisms of Mycobacterium avium MAV-5183 protein on apoptosis in mouse Ana-1 macrophages. A pET-21a-MAV-5183 recombinant plasmid was constructed. The recombinant MAV-5183 protein was cloned, expressed, purified, and identified using an anti-His-tagged antibody.

Preparation of amphiphilic polyquaternium nanofiber films with antibacterial activity via environmentally friendly microfluidic-blow-spinning for green food packaging applications.

Green food packaging plays an important role in environmental protection and sustainable development. Therefore, it is advisable to employ low-energy consumption manufacturing techniques, select environmentally friendly materials, and focus on cost-effectiveness with high production yields during the production process. In this study, an amphiphilic polyquaternium called PBzCl was proposed and synthesized by free radical polymerization of cost-efficient quaternary ammonium salts and methacrylate monomers.

Fabrication of antimicrobial PCL/EC nanofibrous films containing natamycin and trans-cinnamic acid by microfluidic blow spinning for fruit preservation.

Fruit is susceptible to various postharvest pathogens; thus, the development of multifunctional preservation materials that can achieve the broad-spectrum inhibition of different pathogens is a current research hotspot. Here, microfluidic blow spinning was used to create a biodegradable polycaprolactone/ethyl cellulose (PCL/EC) nanofibrous film that incorporated two naturally-sourced compounds, natamycin and trans-cinnamic acid, resulting in multi-microbial inhibition. The PCL/EC-based film had a smooth and even morphology, indicating the favorable integration of PCL and EC.

Corrigendum to 'Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix' [Materials Today Bio 12 (2021) 100161].

[This corrects the article DOI: 10.1016/j.mtbio.

Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix.

Acellular adipose matrix (AAM) has emerged as an important biomaterial for adipose tissue regeneration. Current decellularization methods damage the bioactive components of the extracellular matrix (ECM), and the residual immunogenic antigens may induce adverse immune responses. Here, we adopted a modified decellularization method which can protect more bioactive components with less immune reaction by methoxy polyethylene glycol (mPEG).