Dual-Functional Nanoparticles to Reverse Osteoblast Senescence and Enhance Calcium Supplementation for Alleviating Senile Osteoporosis.

Senile osteoporosis (SOP) primarily arises from an imbalance between bone formation and bone resorption. The tightly regulated coupling between osteoblasts and osteoclasts limits the therapeutic efficacy of conventional anti-resorptive agents and anabolic agents. Anti-aging therapy offers a potential strategy to modify the senescent phenotype of bone-associated cells, restore cellular function, and re-establish homeostasis between bone resorption and formation.

Microglia membrane-mediated trans-blood-brain barrier prodrug micelles enhance phagocytosis for glioblastoma chemo-immunotherapy.

Glioblastoma-associated macrophages & microglia (GAMs) are critical immune cells within the glioblastoma (GBM) microenvironment. Their phagocytosis of GBM cells is crucial for initiating both innate and adaptive immune responses. GBM cells evade this immune attack by upregulating the anti-phagocytic molecule CD47 on their surface.

pH-Responsive Biomimetic Polymeric Micelles as Lymph Node-Targeting Vaccines for Enhanced Antitumor Immune Responses.

Lymph nodes are proposed as the intriguing target in cancer immunotherapy, and cellular immunity is vital for vaccines to fight against cancer. However, inefficient delivery of vaccines to lymph nodes and deficient lysosomal escape of antigens result in weak cellular immunity, which restrains the strength of vaccines in inducing antitumor immune responses. Hence, dendritic cell membrane (DCM)/histidine-modified stearic acid-grafted chitosan (HCtSA)/ovalbumin (OVA) micelles, as pH-responsive biomimetic vaccines, were fabricated to target lymph nodes and induce cellular immunity for enhanced antitumor immune responses.

Biomechanical bearing-based typing method for osteonecrosis of the femoral head: ABC typing.

Type classification of osteonecrosis of the femoral head (ONFH) is important for collapse prediction in ONFH, which depends on a complexity of factors. At present, most typing is based on single factors, including the location or size of the necrosis, or the bone repair capacity after ONFH, and is therefore limited. The present study proposes an 'ABC' method for ONFH typing based on biomechanics and the stress distribution characteristics of the femoral head's bone trabeculae.

The influence of traditional Chinese medicine constitutions on the potential repair capacity after osteonecrosis of the femoral head.

Objectives: To explore the effects of traditional Chinese medicine (TCM) constitutions on repair capacity after osteonecrosis of the femoral head (ONFH).

Design And Setting: Ninety-six patients with TCM constitutions of yang-deficiency, dampness-heat and/or blood-stasis were recruited from the orthopedic outpatient clinic of the Guang'anmen Hospital of the China Academy of Chinese Medical Science. Each patient underwent computed tomography (CT) examination of the hips, and CAD software was used to estimate proximal sclerosis ratios based on the CT images.

[Study on the Distribution of Constitutions of Chinese Medicine in Patients with Osteonecrosis of Femoral Head].

Objective: To observe the distribution of constitution types of Chinese medicine (CM) in patients with osteonecrosis of femoral head (ONFH).

Methods: Totally 130 ONFH patients were recruited. Constitution types of CM were identified in all patients.

Prediction of osteonecrosis collapse of the femoral head based on the proportion of the proximal sclerotic rim.

Purpose: The purpose of this study was to predict ONFH-induced collapse based on the percentage of the proximal sclerotic rim.

Methods: In total, 101 patients satisfying the inclusion criteria who received treatment at Guang'anmen Hospital were enrolled. Bilateral hip-joint computed tomography (CT) of the necrotic tissue was performed, and the largest layer within the coronal CT images was selected together with its anterior and posterior layers to calculate the proportion of the proximal sclerotic rim.

Determination of antazoline hydrochloride in Beagle dog plasma by HPLC-UV and its application to pharmacokinetics.

In order to evaluate the pharmacokinetics characteristic of antazoline hydrochloride in Beagle dogs, a sensitive and specific HPLC method was developed and validated using phenacetin as the internal standard (IS). The analyte and the IS were extracted from dog plasma by ethyl acetate under the basic condition. The analyte was separated by a C18 column and detected with a variable wavelength UV-detector.