Exposure of A549 and J774A.1 Cells to SiO and TiO Nanoforms and Related Cellular- and Molecular-Level Effects: Application of Proteomics.

There is an emerging interest in incorporating proteomic data for environmental health risk assessments. Meanwhile, the production and use of engineered nanomaterials (ENMs) with attractive physicochemical properties are expanding with the potential for exposure, thus necessitating toxicity information on these materials for health risk analysis, where proteomic data can be informative. Here, cells (A549 human lung epithelial and J774A.

Survival and virulence of Acinetobacter baumannii in microbial mixtures.

Acinetobacter species such as A. venetianus and A. guillouiae have been studied for various biotechnology applications, including bioremediation of recalcitrant and harmful environmental contaminants, as well as bioengineering of enzymes and diagnostic materials.

Rapid, Sensitive, and Selective Quantification of Spores Using xMAP Technology.

is a spore-forming ubiquitous bacterium notable as a food poisoning agent. Detection of spores using selective media is laborious and non-specific. Herein, the quantitative detection of spores was investigated with commercial antibodies and published aptamer sequences.

Inhibition of cell division in mouse B-cell hybridomas: an overlooked property of 2-mercaptoethanol and its impact on in vitro antibody production.

Thiol 2-mercaptoethanol (2-ME) has been reported to enhance growth in lymphocytes by various investigators. Some have used 50 μM for growing hybridomas in vitro. Concentrations of 50 and 5 μM in 5% FBS supplemented D-MEM were tested to determine their effects on the growth of 5 monoclonal antibody secreting mouse B cell hybridomas and the myeloma Sp2/O-Ag14.