Gut microbiota and metabolites related intra-patient variability of tacrolimus pharmacokinetics predicted adverse one-year outcomes following kidney transplantation.

Kidney transplantation (KT) is an effective treatment for end-stage renal disease, with over 90 % of recipients requiring lifelong tacrolimus (Tac). However, The Tac pharmacokinetics exhibit high intra-patient variability (IPV), posing significant challenges. This study included 102 KT recipients at our center from October 2022 to December 2023.

Prediction of postoperative infection through early-stage salivary microbiota following kidney transplantation using machine learning techniques.

Kidney transplantation (KT) is an effective treatment for end-stage renal disease; however, the lifelong immunosuppressive regimen increases the risk of infection, presenting significant clinical, and economic challenges. Identifying predictive biomarkers for infection onset is critical. In this study, 122 postoperative saliva samples from 39 KT recipients were analyzed using 16S rRNA sequencing, with 16 developing infections within one year.

Automatic Radiology Report Generation Based on State-Space Model.

The objective of radiology report generation is to alleviate the burden on physicians in drafting reports, thereby improving generation efficiency and reducing patient waiting times. In recent years, there has been a growing emphasis on imaging-based monitoring technologies within healthcare, with a particular focus on the precise detection and interpretation of subtle changes. Likewise, as individual X-rays often exhibit minimal differences, pathologies are frequently concealed within intricate details, making accurate report generation a challenge.

Prediction of peripheral blood lymphocyte subpopulations after renal transplantation.

Immune monitoring is essential for maintaining immune homeostasis after renal transplantation (RT). Peripheral blood lymphocyte subpopulations (PBLSs) are widely used biomarkers for immune monitoring, yet there is no established standard reference for PBLSs during immune reconstitution post-RT. PBLS data from stable recipients at various time points post-RT were collected.

Unraveling salivary microbiota diversity following kidney transplantation: insights from baseline peripheral blood lymphocyte subsets.

Background: Effective biomarkers are urgently needed to monitor immune suppression in kidney transplantation (KT) recipients. Our study identified a close association between the salivary microbiota and immunosuppressant concentrations. It is therefore hypothesized that the salivary microbiota may be linked to immune function.

Prediction of delayed graft function by early salivary microbiota following kidney transplantation.

Delayed graft function (DGF) is a frequently observed complication following kidney transplantation (KT). Our prior research revealed dynamic shifts in salivary microbiota post-KT with immediate graft function (IGF), yet its behavior during DGF remains unexplored. Five recipients with DGF and 35 recipients with IGF were enrolled.

Association between salivary microbiota and renal function in renal transplant patients during the perioperative period.

Introduction: Renal transplantation is an effective treatment for the end stage renal disease (ESRD). However, how salivary microbiota changes during perioperative period of renal transplant recipients (RTRs) has not been elucidated.

Methods: Five healthy controls and 11 RTRs who had good recovery were enrolled.

Vasectomy reversal in China during the recent decade: insights from a multicenter retrospective investigation.

To date, there is little information about the demography of vasectomy reversal (VR) patients or the factors currently influencing VR effectiveness in China, especially after the universal two-child policy was released in 2015. In this research, demographic data and perioperative medical records of VR patients were extracted from seven major hospitals in different provinces or municipalities of China. Meanwhile, a telephone survey of the patients was conducted to collect follow-up information.

Urethral calculi with a urethral fistula: a case report and review of the literature.

Background: To explore and summarize the reasons why urethral calculi cause a urethral fistula.

Case Presentation: We retrospectively studied 1 patient in Xiangya hospital and all relevant literature published in English between 1989 and 2015. The patients (including those reported in the literature) were characterized by age, origin, location of calculus, size of calculus, fistulous track, and etiological factors.

[Effect of calcium channel blockers on primary cultured human urethra scar fibroblasts].

To investigate effects of verapamil on primary cultured human urethral scar fibroblasts (USFs) and to provide basis for protecting the formation of urethra scar.
 Methods: The cell proliferation was evaluated with the cell counting kit (CCK)-8 method after USFs were incubated various verapamil concentrations (50, 100, 150, 200, or 250 μmol/L) or solvent for 12, 24, or 48 h. The protein level of matrix metalloproteinase (MMP) was evaluated with ELISA after cells were incubated with verapamil (100 μmol/L) or solvent (control cells) for 24 h.

Multiple sequence alignment algorithm based on a dispersion graph and ant colony algorithm.

In this article, we describe a representation for the processes of multiple sequences alignment (MSA) and used it to solve the problem of MSA. By this representation, we took every possible aligning result into account by defining the representation of gap insertion, the value of heuristic information in every optional path and scoring rule. On the basis of the proposed multidimensional graph, we used the ant colony algorithm to find the better path that denotes a better aligning result.

Coronavirus phylogeny based on 2D graphical representation of DNA sequence.

A novel coronavirus has been identified as the cause of the outbreak of severe acute respiratory syndrome (SARS). Previous phylogenetic analyses based on sequence alignments show that SARS-CoVs form a new group distantly related to the other three groups of previously characterized coronaviruses. In this aritcle, a new approach based on the 2D graphical representation of the whole genome sequence is proposed to analyze the phylogenetic relationships of coronaviruses.