Biomolecular Condensate-Based Artificial Organelle for Driving Compartmentalized Flux Control.

Microbial cell factories have emerged as versatile bioreactors capable of orchestrating complex metabolic networks to convert renewable feedstocks into high-value biochemicals. Nevertheless, the diffusion-mediated dispersion of metabolic intermediates often compromises biosynthesis efficiency, primarily attributable to the absence of artificial subcellular compartments for spatiotemporal organization of catalytic enzymes. Herein, we established a synthetic biology platform leveraging engineered biomolecular condensates to achieve precise flux control via a modular pathway compartmentalization.

Mast cell activation induced by tamoxifen citrate via MRGPRX2 plays a potential adverse role in breast cancer treatment.

Breast cancer is the most common malignant tumor endangering women's life and health. Tamoxifen citrate (TAM) is the first-line drug of adjuvant endocrine therapy for estrogen receptor-positive (ER) breast cancer patients. Some sporadic cases have described rare adverse reactions of TAM with potentially life-threatening dermatological manifestations, which were associated with skin allergy.

Examination of spatial and temporal evolution characteristics of carbon emission and influencing factors in territorial spatial functional areas: a case study of the mountainous city Chongqing.

The rapid development of China's economy and the acceleration of the urbanization process have led to a significant increase in carbon emissions, and more effective policies are urgently needed. As the first city in China to be approved for the overall master plan of territorial space, Chongqing is facing new opportunities in urban construction. This research constructed a classification system of the territorial space functional areas in Chongqing (CQ-TSFA) and matched the corresponding carbon emission and carbon sequestration projects.

Cell membrane chromatography relative competitive method for the accurate determination of relative K values of drug-receptor interactions.

The specific binding of a drug to the receptor is a prerequisite for its action. The equilibrium dissociation constant (K) is an important parameter for measuring the strength of drug-receptor interactions. Cell membrane chromatography (CMC) is a powerful way to determine the K value; however, the common disadvantage is that the attenuation of biological activity with the analysis process leads to corresponding errors in comparing K values of a series of drugs.

Quantity and variety of food groups consumption and the risk of hypertension in adults: a prospective cohort study.

The prospective association of dietary diversity and different food groups with the risk of hypertension in the general Chinese adults has not been evaluated. We aimed to evaluate the relationship of variety and quantity of 12 major food groups with new-onset hypertension in the Chinese adults. A total of 11,118 adults from the China Health and Nutrition Survey (CHNS) were included.

Expanding the cell quantity of CRISPR/Cas9 gene editing by continuous microfluidic electroporation chip.

CRISPR/Cas9-mediated gene editing offers promising and safe therapeutic options for a wide range of diseases. The technical difficulty of efficiently acquiring large quantities of gene-edited therapeutic cells in a short time period is now preventing the widespread clinical application of CRISPR/Cas9-mediated gene editing. Herein, a Large Volume Continuous Electroporation Chip (LaViE-Chip) has been developed to address the challenge of acquiring sufficient quantities of genetically edited cells for CRISPR/Cas9 gene editing.

Six-year change in high-sensitivity cardiac troponin T with subsequent stroke risk in the general population.

Background: The association between change in high-sensitivity cardiac troponin T (hs-cTnT) and stroke risk in the general population remains unknown. We aimed to assess the association of a 6-year change in hs-cTnT with incident stroke and its subtypes in the general American adult population.

Methods: 8675 middle-aged adults without prevalent cardiovascular disease from the Atherosclerosis Risk in Communities study were included.

Pseudolaric Acid B Inhibits FLT4-induced Proliferation and Migration in Non-small Cell Lung Cancer.

Objectives: Non-Small Cell Lung Cancer (NSCLC) has attracted much attention on account of the high incidence and mortality of cancers. Vascular Endothelial Growth Factor Receptor 3 (VEGFR3/FLT4), which is a highly expressed receptor in NSCLC, greatly regulates cancer proliferation and migration. Pseudolaric Acid B (PAB) is a diterpenoid acid with antitumor activity isolated from This study aimed to explore the inhibitory effect of PAB targeting FLT4 in NSCLC.

Association between longitudinal changes in body composition and the risk of kidney outcomes in participants with overweight/obesity and type 2 diabetes mellitus.

Aims: To assess the relationship of longitudinal changes in fat mass (FM), lean mass (LM) and waist circumference (WC) with incident kidney outcomes in people with overweight/obesity and type 2 diabetes mellitus (T2DM).

Materials And Methods: A total of 3927 participants with baseline estimated glomerular filtration rate (eGFR) ≥60 mL/min/1.73 m from the Look AHEAD (Action for Health in Diabetes) trial were included.

In vitro pharmacokinetic behavior in lung of harringtonine, an antagonist of SARS-CoV-2 associated proteins: New insights of inhalation therapy for COVID-19.

Background: Recent studies have shown that harringtonine (HT) could specifically bind with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein and host cell transmembrane serine protease 2 (TMPRSS2) to block membrane fusion, which is an effective antagonist for SARS-CoV-2.

Purpose: Our study focused on in-depth exploration of in vitro pharmacokinetic characteristics of HT in lung.

Methods: HPLC-fluorescence detection method was used to detect changes of HT content.

Incidence, risk and prognosis of second primary malignancy of patients with gastric adenocarcinoma.

Due to the long-term low survival rates of gastric adenocarcinoma (GAC) patients, the occurrence and prognosis of second primary malignancies (SPMs) are often underreported and overlooked as a significant concern.To date, only a few studies have addressed this issue in the context of GAC. These studies, however, are limited by their small patient cohorts and lack of substantial, meaningful findings.

J-shaped association between dietary thiamine intake and the risk of cognitive decline in cognitively healthy, older Chinese individuals.

Background: The prospective association of dietary thiamine intake with the risk of cognitive decline among the general older adults remains uncertain.

Aims: To investigate the association between dietary thiamine intake and cognitive decline in cognitively healthy, older Chinese individuals.

Methods: The study included a total of 3106 participants capable of completing repeated cognitive function tests.

Dauricine Inhibits Non-small Cell Lung Cancer Development by Regulating PTEN/AKT/mTOR and Ras/MEK1/2/ERK1/2 Pathways in a FLT4-dependent Manner.

Objective: Non-small cell lung cancer (NSCLC) is still a solid tumor with high malignancy and poor prognosis. Vascular endothelial growth factor receptor 3 (FLT4, VEGFR3) is overexpressed in NSCLC cells, making it a potential target for NSCLC treatment. In this study, we aimed to explore the anti-cancer effects of dauricine on NSCLC cells and its mechanism targeting FLT4.

L-shaped association between dietary zinc intake and cognitive decline in Chinese older people.

Background And Objective: The prospective association between dietary zinc (Zn) intake and cognitive decline remains uncertain. We aimed to assess the relationship of dietary Zn intake with the risk of cognitive decline in the Chinese older people, and examine the possible effect modifiers on this association.

Methods: A total of 3,106 older Chinese adults aged 55 years or older from China Health and Nutrition Survey were included.

HPLC-fluorescence detection for stability of harringtonine, and identification of degradation products by UPLC-Q-TOF-MS.

Harringtonine (HT) is an anticancer alkaloid early extracted and isolated from cephalotaxus fortunei Hook. f., also has various pharmacological activities such as antiviral, antibacterial, antimalarial, anti-inflammatory, antioxidant, herbicidal and insecticidal.

Association of Body Weight Time in Target Range With the Risk of Kidney Outcomes in Patients With Overweight/Obesity and Type 2 Diabetes Mellitus.

Objective: We assessed the relationship of body weight time in target range (TTR) with composite kidney outcome in people with overweight/obesity and type 2 diabetes mellitus (T2DM).

Research Design And Methods: Included in this study were 3,601 participants with baseline estimated glomerular filtration rate (eGFR) ≥60 mL/min/1.73 m2 from the Look AHEAD (Action for Health in Diabetes) trial.

Polysaccharide from walnut green husk alleviates liver inflammation and gluconeogenesis dysfunction by altering gut microbiota in ochratoxin A-induced mice.

Walnut green husk polysaccharides (WGP) are isolated from the walnut green husk with a mean molecular weight of 12.77 kDa. The structural characterization revealed by methylation and NMR analysis indicated that WGP might consist of →4-α-D-Galp-(1→, α-D-Galp (1→, and →2)-α-L-Rhap-(1→.

Research Progress of Dihydroquercetin in the Treatment of Skin Diseases.

Skin is a barrier to maintaining the stability of the human environment and preventing the invasion of pathogens. When skin tissue is exposed to the external environment, it will inevitably develop defects due to trauma, injury, burns, ulcers, surgery, and chronic diseases. Rapid skin repair is the key to reducing infection, relieving pain, and improving quality of life.

Lam.: a comprehensive review on active components, health benefits and application.

Lam. is an edible therapeutic plant that is native to India and widely cultivated in tropical countries. In this paper, the current application of was discussed by summarizing its medicinal parts, active components and potential mechanism.

Temperature and phosphorus: the main environmental factors affecting the seasonal variation of soil bacterial diversity in Nansi Lake Wetland.

Introduction: The soil bacteria promote the circulation conversion of lake nutrients and play an important role in maintaining the balance of the lake ecosystem. Few studies have investigated the association of seasonal variation in bacteria and environmental factors in inland freshwater lake wetlands. Nansi Lake is a large shallow freshwater lake in northern China.

Dietary low- and high-quality carbohydrate intake and cognitive decline: A prospective cohort study in older adults.

Background & Aims: The association of dietary intake of carbohydrate (CHO), especially high- and low-quality CHO, with the decline of cognitive function remains uncertain. We aimed to investigate the prospective association of dietary total, low- and high-quality CHO intake with cognitive decline, and further examine the effect of isocaloric substitution with protein or fat, in the elderly population.

Methods: A total of 3106 Chinese participants aged ≥55 years from China Health and Nutrition Survey (CHNS) were included in this study.

Identification and analysis of anti-tumor effective substances in Yangzheng Mixture.

Yangzheng Mixture is a traditional Chinese medicine used in clinical practice as an adjuvant therapy for tumors. However, little is known about its active components in tumor treatment. The purpose of this study was to explore the potential anti-tumor components of Yangzheng Mixture to better promote its clinical application.

Association Between Dietary Copper Intake and Cognitive Decline: A Perspective Cohort Study in Chinese Elderly.

Objective: The association between dietary copper (Cu) intake and cognitive decline remains uncertain. We aim to investigate the longitudinal association of dietary Cu with cognitive decline in Chinese elderly.

Methods: A total of 3,106 Chinese adults aged older than or equal to 55 years from China Health and Nutrition Survey (CHNS) were included.