Diagnostic Pitfalls in Fine Needle Aspiration of Pleomorphic Adenoma: Degenerative Changes in Myoepithelial Cells as Malignant and PLAG1 False Negativity.

Fine needle aspiration (FNA) is widely accepted as an efficient first-line diagnostic tool for evaluating salivary gland lesions. It can accurately diagnose a majority of common benign tumors, including pleomorphic adenoma (PA). However, PA can exhibit a wide range of morphological variations, which may lead to misinterpretation.

An Institutional Experience in Lung Endobronchial Ultrasound-Guided Fine Needle Aspiration: Comparing Risk of Malignancy With WHO Reporting System for Lung Cytopathology.

Introduction: Our institution utilises diagnostic frameworks similar to WHO Reporting System for Lung Cytopathology (WHORSLC) for assessment of lung fine needle aspiration (FNA) specimens. This study reports risk of malignancy (ROM) across diagnostic categories for comparison with the WHORSLC published data.

Methods: A SNOMED search of the electronic pathology database in our institution (01/2022-12/2023) was conducted to retrieve endobronchial ultrasound (EBUS)-guided lung FNA specimens with a concurrent or subsequent surgical biopsy.

Nitrogen-Doped Weathered Coal for the Efficient Adsorption of Lead: Adsorption Performance and Mechanisms.

The development of widely sourced and efficient adsorbents is crucial for the adsorption of lead from wastewater. A novel adsorbent, N-doped weathered coal (NWC), was prepared in this study using weathered coal as the precursor and triethylenetetramine (TETA) as the N-source. The adsorption performance and behavior of Pb(II) on NWC were investigated using batch adsorption experiments.

Performance of Afirma Genomic Sequencing Classifier in Binary Subcategories of Atypia of Undetermined Significance Thyroid Nodules: Single Versus Repeat Diagnosis.

Afirma Genomic Sequencing Classifier (GSC) testing has been utilized for further risk stratification of thyroid nodules categorized as atypia of undetermined significance (AUS). The 2023 Bethesda system subcategorizes AUS diagnosis into AUS with nuclear atypia (AUS-N) and other atypia (AUS-O). The current study aims to determine if performance of GSC testing differs between the two AUS subcategories and between single AUS cohort and repeat AUS cohort.

Telecytology versus conventional rapid on-site evaluation for endobronchial ultrasound-guided fine needle aspiration: a single institution's experience.

Introduction: Telecytology (TC) has the advantage of allowing cytopathologists to remotely support multiple sites rapid on-site evaluation (ROSE) concurrently and represents a potential solution for an increased clinical demand for ROSE. In this study, we share our comparative experience of using TC versus conventional (in-person) ROSE for endobronchial ultrasound-guided fine needle aspiration (EBUS-FNA).

Materials And Methods: We evaluated 475 consecutive cases of EBUS-FNA that underwent TC-ROSE from May 2020 to August 2021 along with 475 consecutive cases which had conventional ROSE from November 2019 to August 2021 at the University of Michigan.

Second opinion for pulmonary and pleural cytology is valuable for patient care.

Introduction: Thoracic cytology can be challenging due to limited procured material or overlapping morphology between benign and malignant entities. In such cases, expert consultation might be sought. This study aimed to characterize all pulmonary and pleural cytology consult cases submitted to our practice and provide recommendations on approaching difficult cases.

Performance of preferentially expressed antigen in melanoma (PRAME) immunohistochemistry for metastatic melanoma in cytology specimens.

Background: Preferentially expressed antigen in melanoma (PRAME) has been introduced as a new melanoma marker and potential target for immunotherapy. While PRAME immunohistochemistry (IHC) is well documented in surgical pathology, similar data in cytology are limited. Metastatic melanoma is frequently diagnosed via cytology samples in which IHC plays an important role.

American Society of Cytopathology Telecytology validation recommendations for rapid on-site evaluation (ROSE).

Telecytology has multiple applications, including rapid onsite evaluation (ROSE) of fine-needle aspiration (FNA) specimens. It can enhance cytopathology practice by increasing productivity, reducing costs, and providing subspecialty expertise in areas with limited access to a cytopathologist. However, there are currently no specific validation guidelines to ensure safe practice and compliance with regulations.

Will Using a Foreign Language Attenuate the Neophobia?

In two experiments, we tested whether using a foreign language attenuates neophobia at the lexical (Experiment 1) and discoursal (Experiment 2) levels in comparison to using a native language. A total of 687 native Chinese speakers participated in Experiment 1, and 693 in Experiment 2. All of them learned English as a foreign language.

Composition and sources of suspended particles in the Pacific Arctic region.

During the 6th (2014) and 7th (2016) Chinese Arctic Expedition (CHINARE), samples of suspended particulate matter (SPM) were collected from both surface (depth: <1.0 m) and subsurface (depth: approximately between 10 and 150 m) waters over the northern shelf of the Bering Sea and in the western Arctic Ocean. To investigate the distribution and sources of organic matter in both the surface water and the vertical profile, the concentration and stable carbon isotopic composition of SPM, particulate organic carbon (POC), and particulate nitrogen (only in surface water samples) were determined, and some particle samples were selected for examination using scanning electron microscopy.

Performance of Afirma genomic sequencing classifier and histopathological outcome in Bethesda category III thyroid nodules: Initial versus repeat fine-needle aspiration.

Background: There is limited data comparing the performance of Afirma Genomic Sequencing Classifier (GSC) in thyroid nodules carrying an initial versus a repeat diagnosis of atypia of undetermined significance (AUS). This study reported an institutional experience in this regard.

Materials And Methods: This retrospective study included consecutive thyroid nodules that had an initial or a repeat AUS diagnosis and had a subsequent GSC diagnostic result (benign or suspicious) from 2017 to 2021.

Cytomorphology of nodular histiocytic/mesothelial hyperplasia.

Nodular histiocytic/mesothelial hyperplasia (NHMH) is a pathologic entity that has not been well characterized in the cytopathology literature. This is unfortunate because if unrecognized, NHMH may be misdiagnosed when encountered in cytology specimens. The aim of this communication is to accordingly alert cytologists about NHMH by means of an illustrative case report.

Core needle biopsy wash as a tool for acquiring additional diagnostic material for laboratory testing.

Aims: To determine if a simple prewash step added to the processing workflow of tissue procurement by a core needle biopsy device will recover enough cells to expand the laboratory testing armamentarium.

Methods: Tissue was obtained from unfixed resection specimens using a core needle device and washed in a buffered solution before fixation. This creates a liquid aliquot from which dislodged cells can be kept and separated from the tissue specimen, the latter of which can then undergo traditional formalin-fixed, paraffin-embedded processing.

Could L2 Lexical Attrition Be Predicted in the Dimension of Valence, Arousal, and Dominance?

The current study attended to predict L2 lexical attrition by means of a Decision Tree model (DT model) in three emotional dimensions, that is, the valence dimension, the arousal dimension, and the dominance dimension. A sample of 188 participants whose L1 was Chinese and L2 was English performed a recognition test of 500 words for measuring the L2 lexical attrition. The findings explored by the Decision Tree model indicated that L2 lexical attrition could be predicted in all the three emotional dimensions in two aspects: (1) among the three emotional dimensions, the valence dimension was the most powerful in predicting L2 lexical attrition, followed successively by the dominance dimension and the arousal dimension; (2) most of the neutral words in the three emotional dimensions were predicted to be inferior to emotional words in L2 attrition.

Will Emotional Effects Modulate L2 Lexical Attrition as they Do in L2 Acquisition?

This study attempted to examine the modulation of emotional effects on L2 lexical attrition. For this purpose, a cross-sectional approach was adopted to analyze emotional effects on L2 lexical attrition with a 500-word vocabulary test taken by 188 Chinese-English bilinguals. As indicated by the results, the modulation of emotional effects on L2 lexical attrition was found to be as active as it was in L2 acquisition; Positive words did not differ from negative words in L2 attrition; All three types of emotional words shared a similar attrition pattern, that is, their attrition went very rapidly within the first 4 years, kept stable between year 5 and year 8, and resumed rapidity after the 9th year, with no significant differences in attrition rate between positive and negative words being detected at any stage.

Pore size directs bone marrow stromal cell fate and tissue regeneration in nanofibrous macroporous scaffolds by mediating vascularization.

In the U.S., 30% of adults suffer joint pain, most commonly in the knee, which severely limits mobility and is often attributed to injury of cartilage and underlying bone in the joint.

The distribution of and biodegradation impact on spilled oil in sediments from Dalian Bay, NE China.

Three cores collected in the area of 16th July 2010 oil spill by box crab in May 2013 and July 2014 at the Dalian Bay have been geochemically characterized to investigate the fate of chemical components in sediments. The total organic carbon, extractable organic matter contents and biomarker compositions have been applied for the differentiation of alien organic matters from in situ ones and evaluation of the biodegradation impact. Multivariate statistical analysis suggests four groups of sediments.

Local pulsatile PTH delivery regenerates bone defects via enhanced bone remodeling in a cell-free scaffold.

Parathyroid hormone (PTH) is currently the only FDA-approved anabolic drug to treat osteoporosis, and is systemically administered through daily injections. A new local pulsatile PTH delivery device was developed from biodegradable polymers to expand the application of PTH from systemic treatment to spatially controlled local bone defect regeneration in this work. This is the first time that local pulsatile PTH delivery has been demonstrated to promote bone regeneration via enhanced bone remodeling.

Nanofibrous spongy microspheres for the delivery of hypoxia-primed human dental pulp stem cells to regenerate vascularized dental pulp.

Unlabelled: Dental pulp infection and necrosis are widespread diseases. Conventional endodontic treatments result in a devitalized and weakened tooth. In this work, we synthesized novel star-shaped polymer to self-assemble into unique nanofibrous spongy microspheres (NF-SMS), which were used to carry human dental pulp stem cells (hDPSCs) into the pulp cavity to regenerate living dental pulp tissues.

Nanofibrous spongy microspheres enhance odontogenic differentiation of human dental pulp stem cells.

Dentin regeneration is challenging due to its complicated anatomical structure and the shortage of odontoblasts. In this study, a novel injectable cell carrier, nanofibrous spongy microspheres (NF-SMS), is developed for dentin regeneration. Biodegradable and biocompatible poly(l-lactic acid)-block-poly(l-lysine) are synthesized and fabricated into NF-SMS using self-assembly and thermally induced phase separation techniques.

Injectable Peptide Decorated Functional Nanofibrous Hollow Microspheres to Direct Stem Cell Differentiation and Tissue Regeneration.

Injectable microspheres are attractive stem cell carriers for minimally invasive procedures. For tissue regeneration, the microspheres need to present the critical cues to properly direct stem cell differentiation. In natural extracellular matrix (ECM), growth factors (GFs) and collagen nanofibers provide critical chemical and physical cues.

Rapid Self-Integrating, Injectable Hydrogel for Tissue Complex Regeneration.

A novel rapid self-integrating, injectable, and bioerodible hydrogel is developed for bone-cartilage tissue complex regeneration. The hydrogels are able to self-integrate to form various structures, as can be seen after dying some hydrogel disks pink with rodamine. This hydrogel is demonstrated to engineer cartilage-bone complex.

Calcium phosphate deposition rate, structure and osteoconductivity on electrospun poly(l-lactic acid) matrix using electrodeposition or simulated body fluid incubation.

Mineralized nanofibrous scaffolds have been proposed as promising scaffolds for bone regeneration due to their ability to mimic both nanoscale architecture and chemical composition of natural bone extracellular matrix. In this study, a novel electrodeposition method was compared with an extensively explored simulated body fluid (SBF) incubation method in terms of the deposition rate, chemical composition and morphology of calcium phosphate formed on electrospun fibrous thin matrices with a fiber diameter in the range ~200-1400 nm prepared using 6, 8, 10 and 12 wt.% poly(l-lactic acid) (PLLA) solutions in a mixture of dichloromethane and acetone (2:1 in volume).