Single-Cell RNA Sequencing Reveals Potential Mechanism of RUNX3 Reshaping Tumor Microenvironment in Non-small-cell Lung Cancer.

Background: RUNX3 acts as a tumor suppressor gene in non-small-cell lung cancer (NSCLC), yet its specific biological mechanism is still unclear. This study aimed to uncover tumor microenvironment (TME) changes in NSCLC with varying RUNX3 expression statuses through single-cell RNA sequencing.

Patients And Methods: In total, seven patients with NSCLC with detailed pathological data were involved, with three both paracancerous and cancerous tissue samples.

Genistein: A promising botanical fungicide candidate for enhancing tomato yield and quality by controlling Alternaria solani.

The overreliance on traditional chemical fungicides, combined with the emergence of resistance, poses significant challenges for food safety. Early blight, caused by the fungal pathogen Alternaria solani (A. solani), is among the most significant contributors to pre- and postharvest yield losses in tomato cultivation.

Myxomycetes in urban green space in subtropical China: Spatiotemporal patterns override forest type effects.

Urban green areas are vital yet underexplored reservoirs of microbial diversity in cities. This study examines myxomycete communities in Zijin Mountain National Forest Park, a subtropical urban forest in Nanjing, China, across four seasons and multiple forest types. Combining field collections and moist chamber cultures, we documented 60 species from 906 occurrence records.

Regulation of food intake by Connexin43 via adipocyte-sensory neuron electrical synapses.

Background And Objective: Connexin43 (Cx43), encoded by Gja1, forms gap junctions between adjacent cells. In adipose tissue, it is upregulated during adipose beiging while downregulated by high-fat-diet (HFD) feeding. Adipocyte-specific Gja1 overexpression enhances adipose tissue beiging in response to mild cold stress of room temperature.