[F]SynVesT-1 PET Detects SV2A Changes in the Spinal Cord and Brain of Rats with Spinal Cord Injury.

Traumatic spinal cord injury (SCI) is a devastating neurologic condition lacking effective prognostic and treatment methods. PET imaging of synaptic vesicle glycoprotein 2A (SV2A) has been used in measuring synapse changes. We explore the feasibility of using [F]SynVesT-1 PET to detect the synaptic changes in a rat model of SCI.

Evaluation of radio-thin-layer chromatography as an alternative to radio-HPLC for [F]SynVesT-1 metabolism analysis in rats.

Purpose: Alterations in synaptic vesicle glycoprotein 2A (SV2A) are linked to various neurodegenerative and neuropsychiatric disorders. Positron emission tomography (PET) imaging with radiotracers targeting SV2A, such as [F]SynVesT-1, has proven effective for monitoring these changes. However, SV2A PET quantification using kinetic modeling requires radiometabolite analysis, which presents challenges, particularly in preclinical longitudinal studies due to the relatively large sample volume required by the standard radio-high-performance liquid chromatography (radio-HPLC) method.

Decreased synaptic vesicle glycoprotein 2A binding in a rodent model of familial Alzheimer's disease detected by [F]SDM-16.

Introduction: Synapse loss is one of the hallmarks of Alzheimer's disease (AD) and is associated with cognitive decline. In this study, we tested [F]SDM-16, a novel metabolically stable SV2A PET imaging probe, in the transgenic APPswe/PS1dE9 (APP/PS1) mouse model of AD and age-matched wild-type (WT) mice at 12 months of age.

Methods: Based on previous preclinical PET imaging studies using [C]UCB-J and [F]SynVesT-1 in the same strain animals, we used the simplified reference tissue model (SRTM), with brain stem as the pseudo reference region to calculate distribution volume ratios (DVRs).