Publications by authors named "Timothy J Johnson"

Reflexivity can be an important tool for building relationships for participatory research. The purpose of this article was to explore key indicators of a developing participatory action research partnership between an occupational therapy education program and a developmental disability agency. Agency staff leaders and occupational therapy faculty participated in a contextual and interpersonal reflexivity process during participatory action research.

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Escherichia coli is a bacterium ubiquitous to the healthy intestinal microflora in animals. Some E. coli can cause disease in poultry, but disease manifestation is a result of the balance between stress level in the bird and the virulence potential of E.

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(ORT) is a Gram-negative, pleomorphic rod-shaped bacterium that causes respiratory disease in the commercial poultry industry. This bacterium has been causing severe disease in chickens and turkeys since it emerged in the 1980s. In birds, increased mortality, reduced egg production, and loss in weight gain gives ORT the potential to have severe economic implications.

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The current case report describes two outbreaks of central nervous system signs associated with subsp. causing encephalitis and meningitis in poults in the U.S.

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, formerly known as , is a Gram-positive coccus bacterium that is a facultative anaerobe. Although it was previously considered a commensal bacterium that caused secondary disease because of a stressor, can now be considered a causative agent of disease associated with a variety of infections in both humans and animals. has become an emerging pathogen in the poultry industry, particularly in turkey poults, causing acute mortality, usually without any premonitory signs.

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Effective postharvest interventions at the processing stages are recommended to control the transmission of emerging Salmonella serotypes from poultry products to humans. This study investigated the efficacy of pimenta essential oil (PEO) as a pre-grind chill dip antibacterial alone or in combination with peracetic acid (PAA) against emerging serotypes of Salmonella (S. Agona and S.

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A Salmonella enterica serovar Abony outbreak occurred during January-April 2024 in Chile. Genomic evidence indicated that the outbreak strain was a clone of reference strain WDCM 00029, which is routinely used in microbiological quality control tests. When rare or unreported serovars cause human infections, clinicians and health authorities should request strain characterization.

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The effects of light scattering and refraction play significantly different roles for aerosols than for bulk materials, making it challenging to identify aerosolized chemicals using traditional spectral methods or spectral reference libraries. Due to a potentially infinite number of particle morphologies, sizes, and compositions, constructing a database of laboratory-measured aerosol spectra is not a practical solution. Here, as an alternative approach, the measured n/k optical vectors of two example organic materials (diethyl phthalate and D-mannitol) are used in combination with particle absorption / scattering theory (Mie theory and FDTD) and the Beer-Lambert law to generate a series of synthetic infrared transmission / scattered light spectra.

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(PM) is a major bacterial pathogen that causes fowl cholera disease in both domestic poultry and wild birds. Here, we report the complete genome sequences of eight PM isolates representing all known lipopolysaccharide outer core loci, which are phenotypically expressed as 16 known PM serotypes.

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Article Synopsis
  • The detection and cleanup of per- and polyfluoroalkyl substances (PFAS) is increasingly important due to their environmental contamination.
  • Researchers are exploring low-temperature thermal treatment (200-500 °C) for PFAS, which requires less energy and infrastructure, but the byproducts of this process are not well understood.
  • Using gas-phase infrared spectroscopy and multivariate curve resolution analysis, the study identifies and quantifies nine decomposition products from the thermal breakdown of potassium perfluorooctanesulfonate (PFOS-K) starting at 375 °C.
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is the leading cause of urinary tract infections (UTIs) in children and adults. The gastrointestinal tract is the primary reservoir of uropathogenic , which can be acquired from a variety of environmental exposures, including retail meat. In the current study, we used a novel statistical-genomic approach to estimate the proportion of pediatric UTIs caused by foodborne zoonotic strains.

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Salmonella enterica serovar Typhimurium is one of the top Salmonella serovars annually linked to poultry production and corresponding human illnesses. Because of this, vaccination of commercial poultry against Salmonella Typhimurium has been a focal point in recent years. There are several commercially available Salmonella Typhimurium vaccines available for use in poultry production.

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Reducing the colonization of in turkeys is critical to mitigating the risk of its contamination at later stages of production. Given the increased susceptibility of newly hatched poults to colonization, it is crucial to implement interventions that target potential transmission routes, including drinking water. As no individual intervention explored to date is known to eliminate , the United States Department of Agriculture-Food Safety Inspection Service (USDA-FSIS) recommends employing multiple hurdles to achieve a more meaningful reduction and minimize the potential emergence of resistance.

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Bacitracin is an antimicrobial used in the feed or water of poultry in the U.S. for the prevention, treatment, and control of clostridial diseases such as necrotic enteritis.

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Article Synopsis
  • In places like Cambodia, where sanitation is poor, bacteria can easily spread between humans and animals, worsening the problem of antibiotic resistance.
  • The study found similar patterns of antibiotic resistance in humans and animals in Cambodia, highlighting the need for better control at the human-animal interface to combat this issue effectively, particularly in lower and middle-income countries.
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Obtaining the complex refractive index vectors (~) and (~) allows calculation of the (infrared) reflectance spectrum that is obtained from a solid in any of its many morphological forms. We report an adaptation to the KBr pellet technique using two gravimetric dilutions to derive quantitative (~)/(~) for dozens of powders with greater repeatability. The optical constants of bisphenol A and sucrose are compared to those derived by other methods, particularly for powdered materials.

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Due to a legacy originating in the limited capability of early computers, the spectroscopic resolution used in Fourier transform infrared spectroscopy and other systems has largely been implemented using only powers of two for more than 50 years. In this study, we investigate debunking the spectroscopic lore of, e.g.

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Background: The spread of antibiotic-resistant bacteria may be driven by human-animal-environment interactions, especially in regions with limited restrictions on antibiotic use, widespread food animal production, and free-roaming domestic animals. In this study, we aimed to identify risk factors related to commercial food animal production, small-scale or "backyard" food animal production, domestic animal ownership, and practices related to animal handling, waste disposal, and antibiotic use in Ecuadorian communities.

Methods And Findings: We conducted a repeated measures study from 2018 to 2021 in 7 semirural parishes of Quito, Ecuador to identify determinants of third-generation cephalosporin-resistant E.

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The coronavirus 2019 (COVID-19) pandemic has had significant impacts on health systems, population dynamics, public health awareness, and antibiotic stewardship, which could affect antibiotic resistant bacteria (ARB) emergence and transmission. In this study, we aimed to compare knowledge, attitudes, and practices (KAP) of antibiotic use and ARB carriage in Ecuadorian communities before versus after the COVID-19 pandemic began. We leveraged data collected for a repeated measures observational study of third-generation cephalosporin-resistant E.

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We developed a course-based undergraduate research experience (CURE) that gives students an opportunity to practice the process of science in a context that intersects with their everyday lives: purchasing grocery store chicken. Student mastery of concepts was assessed by pre- and postassessment questions and lab report worksheets that guided them through the process of writing a scientific paper. Learning to produce graphs from large data sets and comparing the results with published data emphasized quantitative reasoning, while working as a group and writing helped students practice scientific communication.

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Article Synopsis
  • The development of probiotics for production animals has traditionally focused on selecting strains based on observable traits, but recent studies suggest that using host-specific strains may yield better results.
  • Research on Lactobacillus johnsonii in poultry shows a strong link between the bacteria's evolutionary relationship with its animal host and its effectiveness, with naturally isolated strains from chickens and turkeys displaying significant phenotypic variations.
  • In trials, turkey-specific L. johnsonii strains improved early growth in turkey poults, while chicken-specific strains enhanced overall performance in broiler chickens, highlighting the importance of host adaptation in selecting probiotic candidates.
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Article Synopsis
  • A one-health approach suggests new insights into the transmission of pathogenic bacteria responsible for serious infections in humans, which primarily originate from food animals.
  • Research analyzing over 3,100 samples reveals that approximately 8% of extraintestinal infections, mainly urinary tract infections, are linked to foodborne zoonotic strains, which show similar disease severity as non-foodborne strains.
  • Specific lineages of these foodborne strains, ST131-22 and ST58, display high virulence, and this study's methods could help identify and mitigate risks from these strains in future public health efforts.
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Molecular characterization of avian pathogenic Escherichia coli (APEC) is challenging due to the complex nature of its associated disease, colibacillosis, in poultry. Numerous efforts have been made toward defining APEC, and it is becoming clear that certain clonal backgrounds are predictive of an avian E. coli isolate's virulence potential.

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The objective of this systematic review was to compare the efficacy of antibiotic and non-antibiotic alternatives in the prevention and treatment of necrotic enteritis (NE) in broiler chickens. experimental and observational studies that compared the administration of non-antibiotic compounds with antibiotics to prevent or treat NE in broiler chickens and that evaluated mortality and/or clinical or subclinical NE outcome measures were eligible. Four electronic databases were searched in December 2019 and updated in October 2021.

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A spectral analysis tool has been developed to interactively identify and quantify individual gas-phase species from complex infrared absorbance spectra obtained from laboratory or field data. The SpecQuant program has an intuitive graphical interface that accommodates both reference and experimental data with varying resolution and instrumental lineshape, as well as algorithms to readily align the wavenumber axis of a sample spectrum with the raster of a reference spectrum. Using a classical least squares model in conjunction with reference spectra such as those from the Pacific Northwest National Laboratory (PNNL) gas-phase infrared database or simulated spectra derived from the HITRAN line-by-line database, the mixing ratio of each identified species is determined along with its associated estimation error.

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