N-Degron-Based PROTAC Targeting PLK1: A Potential Therapeutic Strategy for Cervical Cancer.

: Cervical cancer remains a major global health concern, with existing chemotherapy facing limited effectiveness owing to resistance. Polo-like kinase 1 (PLK1) overexpression in cervical cancer cells is a promising target for developing novel therapies to overcome chemoresistance and improve treatment efficacy. : In this study, we developed a novel PROTAC, NC1, targeting PLK1 PBD via the N-end rule pathway.

Systematic analysis of cellular cross-talk reveals a role for SEMA6D-TREM2 regulating microglial function in Alzheimer's disease.

Cellular cross-talk, mediated by membrane receptors and their ligands, is crucial for brain homeostasis and can contribute to neurodegenerative diseases such as Alzheimer's disease (AD). To find cross-talk dysregulations involved in AD, we reconstructed cross-talk networks from single-nucleus transcriptional profiles of 67 clinically and neuropathologically well-characterized controls and AD brain donors from the Knight Alzheimer Disease Research Center and the Dominantly Inherited Alzheimer Network cohorts. We predicted a role for TREM2 and additional AD risk genes mediating neuron-microglia cross-talk in AD.

Comparison of rebamipide and tegoprazan combination therapy and tegoprazan monotherapy for ESD induced gastric ulcers a randomized multicenter study.

Currently, no consensus exists on optimal treatment for post-endoscopic submucosal dissection (ESD) gastric ulcer. This study aims to compare rebamipide and tegoprazan combination therapy with tegoprazan monotherapy for post-ESD ulcer healing. Between May 2022 and September 2023, 140 patients (141 lesions) who underwent ESD for gastric epithelial neoplasms at five tertiary hospitals were randomly assigned to tegoprazan monotherapy or combination therapy groups.

Advanced WBG power semiconductor packaging: nanomaterials and nanotechnologies for high-performance die attach paste.

Wide bandgap (WBG) power semiconductors have attracted significant attention from both academia and industry because they are superior to conventional silicon-based devices. In WBG power semiconductor packages, die attach materials play a crucial role in maximizing device performance and reliability. The die attach interfaces in WBG packages must withstand high operating temperatures (200-300 °C), fast switching frequencies, and great power densities while maintaining excellent thermomechanical reliability.

Thunb. exhibits anti-platelet activity via the regulation of cyclic guanosine monophosphate.

Introduction: Traditionally, Thunb. (GJ) extract has been used to treat headaches and dizziness. We hypothesize that GJ exhibits anti-platelet activity that may prevent ischemic events to alleviate these symptoms.

Unveiling Radiation-Tolerant Thickness in a-IGZO Thin-Film Transistors with Sub-10 nm Film and Restoring Abnormalities via Energy-Efficient Electrothermal Annealing.

The threshold voltage () shift and anomalous hump characteristics caused by external gamma-rays (γ-rays) are problematic in electronic devices, especially in aerospace applications. To overcome those issues, we investigated the degradation mechanism and radiation immunity in ultrathin amorphous indium-gallium-zinc oxide (a-IGZO) thin-film transistors (TFTs) with a sub-10 nm film, utilizing X-ray photoelectron spectroscopy (XPS) analysis and subgap density-of-states (DOS) characterization. Furthermore, energy-efficient electrothermal annealing (ETA) with a power-optimized electrical pulse signal is adopted to recover inevitable device damage triggered by continuous irradiation, resulting in enhancement of subthreshold slope (SS), elimination of the abnormal hump phenomenon, and confirmed heat spreading through thermal simulation.

Early, very high-dose, and prolonged vitamin C administration in murine sepsis.

This proof-of-concept study aimed to assess the optimal timing, dosing, and duration of vitamin C administration to increase survival and attenuate organ injuries in murine sepsis. Mice were randomized to receive ascorbic acid (AscA) at 1 or 6 h after cecal ligation and puncture (CLP). At each time point, mice randomly received AscA for 4 or 8 d.

Recycling Motorcycle Exhaust Soot into Fluorescent Graphene Oxide Quantum Dots for Sensing Ferrocyanide Ions and Bioimaging Cells: A Method for Waste Utilization.

Graphene oxide quantum dots (GOQDs) with a high quantum yield (50%) were synthesized using soot collected from a motorcycle (petroleum vehicle) exhaust pipe and applied as sensors for ferrocyanide ([Fe(CN)]) ions and as bioimaging agents in a cancer cell line. X-ray photoelectron spectroscopy (XPS) data for the GOQDs revealed a C/O ratio of 2.49, which was close to that of graphene oxide (GO).

Associations of mechanical power, ventilatory ratio, and other respiratory indices with mortality in patients with acute respiratory distress syndrome undergoing pressure-controlled mechanical ventilation.

Background: Mechanical power (MP) and ventilatory ratio (VR) are crucial metrics in the management of acute respiratory distress syndrome (ARDS). This study aimed to evaluate the impact of these factors on ICU mortality in patients with ARDS undergoing pressure-controlled ventilation.

Methods: In this retrospective study, we included 600 adult patients with ARDS who required mechanical ventilation for > 48 h between March 2018 and February 2021 in a tertiary referral hospital in Korea.

Proteomic Analysis of Aqueous Humor Identified Clinically Relevant Molecular Targets for Neovascular Complications in Diabetic Retinopathy.

Diabetic retinopathy (DR) is a leading cause of blindness in adults under 40 in the developed world, with a significant proportion progressing to vision-threatening stages such as proliferative diabetic retinopathy (PDR) and neovascular glaucoma (NVG). This study aims to explore the molecular mechanisms underlying the progression from nonproliferative DR to PDR and NVG, focusing on identifying potential biomarkers and therapeutic targets. Utilizing discovery-based proteomics, specifically label-free quantification and tandem mass tag, we analyzed aqueous humor (AH) proteins obtained during cataract surgery or anterior chamber paracentesis from patients with nonproliferative DR, PDR, and NVG.

Continuous Tracking for Effective Tackling: Ad5/35 Platform-Based JN1 Lineage Vaccines Development in Response to Evolving SARS-CoV-2 Variants.

The SARS-CoV-2 virus is continuously evolving, such that JN.1 and its subvariants, including KP.2, KP.

Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry Yeast.

Yeast formulations such as dry yeast are essential for supplying microbial starters to the alcoholic beverage industry. In Korea, the expensive freeze-drying method is used to manufacture brewer's dry yeast, and therefore an economical process such as fluidized bed drying is needed. In the dry yeast manufacturing process, the medium and drying conditions are key factors that determine its quality and manufacturing cost.

Rapid and Environment-Friendly LC-MS/MS for Simultaneous Analysis of Amino Acids in Veterinary Medicine.

Background: Amino acid supplements are crucial for animal health and productivity. Traditional analysis methods face limitations like complexity, long testing times and toxic reagents. Therefore, a more efficient and reliable method is needed.

Enhanced yield and subtype identity of hPSC-derived midbrain dopamine neuron by modulation of WNT and FGF18 signaling.

While clinical trials are ongoing using human pluripotent stem cell-derived midbrain dopamine (mDA) neuron precursor grafts in Parkinson's disease (PD), current protocols to derive mDA neurons remain suboptimal. In particular, the yield of TH+ mDA neurons after grafting and the expression of some mDA neuron and subtype-specific markers can be further improved. For example, characterization of mDA grafts by single cell transcriptomics has yielded only a small proportion of mDA neurons and a considerable fraction of contaminating cell populations.

Development of a highly efficient microbial fermentation process of recombinant Escherichia coli for GABA production from glucose.

This study was aimed to develop a highly productive microbial fermentation process for gamma-aminobutyric acid (GABA) production from glucose. For this, an efficient GABA-producing E. coli strain was firstly developed through metabolic engineering with a strategy of increasing the flux of GABA biosynthetic pathway and deleting or repressing the GABA shunt pathways that compete with GABA biosynthesis.

Machine learning analysis of population-wide plasma proteins identifies hormonal biomarkers of Parkinson's Disease.

As the number of Parkinson's patients is expected to increase with the growth of the aging population there is a growing need to identify new diagnostic markers that can be used cheaply and routinely to monitor the population, stratify patients towards treatment paths and provide new therapeutic leads. Genetic predisposition and familial forms account for only around 10% of PD cases [1] leaving a large fraction of the population with minimal effective markers for identifying high risk individuals. The establishment of population-wide omics and longitudinal health monitoring studies provides an opportunity to apply machine learning approaches on these unbiased cohorts to identify novel PD markers.

Low-Frequency Noise Related to the Scattering Effect in p-Type Copper(I) Oxide Thin-Film Transistors.

In this study, we investigate the origins of low-frequency noise (LFN) and 1/ noise in CuO thin-film transistors (TFTs). The static direct current (DC) - characterization demonstrates that the channel resistance () contributes significantly to mobility degradation in the TFTs, with channel thickness () controlled through the plasma-enhanced atomic layer deposition (PEALD) process. The 1/ noise followed the Hooge mobility fluctuation (HMF) model, and it was observed that both Coulomb and phonon scattering within the channel, which increased with a decrease in , contributed simultaneously.

Robust and inducible genome editing via an all-in-one prime editor in human pluripotent stem cells.

Prime editing (PE) allows for precise genome editing in human pluripotent stem cells (hPSCs), such as introducing single nucleotide modifications, small insertions or deletions at a specific genomic locus. Here, we systematically compare a panel of prime editing conditions in hPSCs and generate a potent prime editor, "PE-Plus", through co-inhibition of mismatch repair and p53-mediated cellular stress responses. We further establish an inducible prime editing platform in hPSCs by incorporating the PE-Plus into a safe-harbor locus and demonstrated temporal control of precise editing in both hPSCs and differentiated cells.

Cellular internalization, cytotoxicity and DNA binding property of 2,3-diaminophenazine, synthesized using Jeanbandyite, a heterogeneous catalyst.

In recent years, the development of novel chemistry routes for the synthesis of organic compounds has attracted special attention. 2,3-Diaminophenazine (DAP), a derivative of Phenazine, is a large group of nitrogen-containing heterocyclic compound with diverse chemical structure and various biological activities, such as antibacterial, antimicrobial, anti-inflammatory, and anticancer activities. Phenazine is a fluorescent molecule with wide range of biological properties.

Development of a Rapid Detection Method for Ethylene Glycol and Glycolic Acid in Feline Samples: A Response to Increasing Antifreeze Poisoning Incidents in Korea.

Recently, cases of antifreeze poisoning in companion animals, particularly cats, have surged in the Republic of Korea. Ethylene glycol (EG), the toxic primary component of antifreeze, is metabolized into glycolic acid (GA), leading to severe metabolic acidosis, acute kidney injury, and death. Traditional detection methods, although effective, are often time-consuming owing to complex sample preparation.