Genome analysis of serovar enteritis strains isolated from poultry and humans in Burkina Faso.

Whole-genome sequencing (WGS) was used to characterize four Enteritidis isolates from poultry () and human () from Ouagadougou, Burkina Faso. Antimicrobial resistance genes, chromosomal mutations, and mobile genetic elements were identified by analysis of WGS data using sequence homology.

A Genetic Locus in Associated with Elevated Vancomycin Resistance and Multiple Antibiotic Reduced Susceptibility.

The Gram-negative express multiple antibiotic resistance and cause severe opportunistic infections. Vancomycin is commonly used to treat Gram-positive infections and has also been used to treat infections, even though Gram-negative organisms possess a vancomycin permeability barrier. appeared relatively vancomycin-susceptible and challenge with this drug led to morphological changes indicating cell lysis.

Resistance Genes, Plasmids, Multilocus Sequence Typing (MLST), and Phenotypic Resistance of Non-Typhoidal (NTS) Isolated from Slaughtered Chickens in Burkina Faso.

The emergence of antimicrobial-resistant bacteria in developing countries increases risks to the health of both such countries' residents and the global community due to international travel. It is consequently necessary to investigate antimicrobial-resistant pathogens in countries such as Burkina Faso, where surveillance data are not available. To study the epidemiology of antibiotic resistance in , 102 strains isolated from slaughtered chickens were subjected to whole-genome sequencing (WGS) to obtain information on antimicrobial resistance (AMR) genes and other genetic factors.

(Adenine Phosphoribosyltransferase) Mutation in Laboratory-Selected Vancomycin-Intermediate .

Comparative genomic sequencing of laboratory-derived vancomycin-intermediate (VISA) (MM66-3 and MM66-4) revealed unique mutations in both MM66-3 (in and ), and MM66-4 (in and ), compared to hetero-VISA parent strain MM66. Transcriptional profiling revealed that both MM66 VISA shared 79 upregulated genes and eight downregulated genes. Of these, 30.

Serotyping of sub-Saharan Africa Salmonella strains isolated from poultry feces using multiplex PCR and whole genome sequencing.

Background: Salmonella enterica remains a leading cause of food-borne diseases worldwide. Serotype information is important in food safety and public health activities to reduce the burden of salmonellosis. In the current study, two methods were used to determine serotypes of 111 strains of Salmonella isolated from poultry feces in Burkina Faso.

Carriage and Gene Content Variability of the pESI-Like Plasmid Associated with Infantis Recently Established in United States Poultry Production.

Infantis carrying extended spectrum β-lactamase on a pESI-like megaplasmid has recently emerged in United States poultry. In order to determine the carriage rate and gene content variability of this plasmid in U.S.

Comparison of Antimicrobial Resistance and Pan-Genome of Clinical and Non-Clinical from Poultry Using Whole-Genome Sequencing.

is an emerging avian pathogen, particularly in chickens, but can be found in both diseased (clinical) and healthy (non-clinical) poultry. To better define differences between E. cecorum from the two groups, whole-genome sequencing (WGS) was used to identify and compare antimicrobial resistance genes as well as the pan-genome among the isolates.

Circulation of emerging NDM-5-producing Escherichia coli among humans and dogs in Egypt.

The emergence of NDM-producing Escherichia coli has considerably threatened human and animal health worldwide. This study describes for the first time in Egypt, the draft genome sequences of emerging NDM-5-producing E. coli from humans and dogs, and investigates genetic relatedness between isolates from both sources.

Genomic Analysis of Multidrug-Resistant from Surface Water in Northeast Georgia, United States: Presence of an ST131 Epidemic Strain Containing on a Phage-Like Plasmid.

Surface water is suspected of playing a role in the development and spread of antimicrobial-resistant (AR) bacteria, including human pathogens. In our previous study, 496 isolates were recovered from water samples collected over a 2-year period from the Upper Oconee watershed, Athens, GA, United States, of which 34 (6.9%) were AR isolates.

Genomic comparison of diverse Salmonella serovars isolated from swine.

Food animals act as a reservoir for many foodborne pathogens. Salmonella enterica is one of the leading pathogens that cause food borne illness in a broad host range including animals and humans. They can also be associated with a single host species or a subset of hosts, due to genetic factors associated with colonization and infection.

Genome Analysis of Multidrug-Resistant Isolated from Poultry in Nigeria.

is one of the most common commensal bacteria of the gastrointestinal tract of humans and warm-blooded animals. Contaminated poultry can lead to disease outbreaks in consumers causing massive economic losses in the poultry industry. Additionally, commensal can harbor antibiotic resistance genes that can be transferred to other bacteria, including pathogens, in a colonized human host.

Antimicrobial Resistance Genes, Cassettes, and Plasmids Present in Associated With United States Food Animals.

The ability of antimicrobial resistance (AR) to transfer, on mobile genetic elements (MGEs) between bacteria, can cause the rapid establishment of multidrug resistance (MDR) in bacteria from animals, thus creating a foodborne risk to human health. To investigate MDR and its association with plasmids in , whole genome sequence (WGS) analysis was performed on 193 isolated from sources associated with United States food animals between 1998 and 2011; 119 were resistant to at least one antibiotic tested. Isolates represented 86 serotypes and variants, as well as diverse phenotypic resistance profiles.

An assay for determining the susceptibility of Salmonella isolates to commercial and household biocides.

Poultry and meat products contaminated with Salmonella enterica are a major cause of foodborne illness in the United States. The food industries use a wide variety of antimicrobial interventions to reduce bacterial contamination. However, little is known about Salmonella susceptibility to these compounds and some studies have shown a concerning link between biocide resistance and antibiotic resistance.

Draft genome sequence of a human-associated streptogramin-resistant Staphylococcus aureus.

Objectives: Staphylococcus aureus is one of the leading causes of nosocomial and community-acquired infections. Treatment of these infections with macrolide-lincosamide-streptogramin (MLS) antibiotics has led to resistance to these antibiotics via various mechanisms. S.

Multidrug resistant Mannheimia haemolytica isolated from high-risk beef stocker cattle after antimicrobial metaphylaxis and treatment for bovine respiratory disease.

Antimicrobial resistance (AMR) in bacterial respiratory pathogens in high-risk stocker cattle has been poorly characterized. The objective of this study was to describe the prevalence of multidrug resistant (MDR; resistance to > 3 antimicrobial classes) respiratory pathogens in 50 conventionally managed stocker cattle over 21 days after arrival. Cattle received tildipirosin metaphylaxis on day 0 and were eligible to receive up to 3 additional antimicrobials for bovine respiratory disease (BRD): florfenicol, ceftiofur and enrofloxacin.

Draft genome sequences of two ciprofloxacin-resistant Salmonella enterica subsp. enterica serotype Kentucky ST198 isolated from retail chicken carcasses in Egypt.

Objectives: Salmonella enterica serotypes, particularly antimicrobial-resistant strains, pose a major threat to public health worldwide. This study describes the draft genome sequences of two ciprofloxacin-resistant Salmonella enterica subsp. enterica serotype Kentucky isolates (H5 and H18) recovered from chicken carcass rinsates in Mansoura, Egypt.

In Silico Prediction of Antibiotic Resistance in Agy99 through Whole Genome Sequence Analysis.

Buruli ulcer is an emerging infectious disease caused by that has been reported from 33 countries. Antimicrobial agents either alone or in combination with surgery have been proved to be clinically relevant and therapeutic strategies have been deduced mainly from the empirical experience. The genome sequences of .

Draft Genome Sequence of Salmonella enterica subsp. diarizonae Serovar 61:k:1,5,(7) Strain CRJJGF_00165 (Phylum Gammaproteobacteria).

Here, we report a 4.78-Mb draft genome sequence of the Salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) strain CRJJGF_00165 [also called S.

Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Orion Strain CRJJGF_00093 (Phylum Gammaproteobacteria).

Here, we report a 4.70-Mbp draft genome sequence of Salmonella enterica subsp. enterica serovar Orion strain CRJJGF_00093, isolated from a dog in 2005.

Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Bardo Strain CRJJGF_00099 (Phylum Gammaproteobacteria).

Here, we report a 4.87-Mbp draft genome sequence of the multidrug-resistant (MDR) Salmonella enterica subsp. enterica serovar Bardo strain CRJJGF_00099, isolated from dairy cattle in 2005.