Identification and Characterization of PLUTO-201, a Novel Long Non-Coding RNA Associated with Poor Outcomes in Prostate Cancer.

Unlabelled: Despite extensive investigation, the factors promoting aggressive prostate cancer are poorly understood. By performing a comprehensive analysis of whole-genome transcriptome data to identify differential expression across 1,567 patients with prostate cancer, we now report the identification of a novel lncRNA, Prostate Locus of Uncharacterized Transcript Outlier 201 (PLUTO-201), which is strongly associated with metastasis and poor overall survival in men with prostate cancer. We find that overexpression/knockdown of PLUTO-201 in pre-clinical models of prostate cancer modulates proliferation rates and markers of an aggressive phenotype through regulation of steroid biosynthesis and expression of the MHC class I complex, driving increased growth in androgen-depleted conditions and decreased susceptibility to T cell-mediated cytotoxicity.

Extending the Coverage of IEEE 802.15.4z HRP UWB Ranging.

Currently, the ultra-wideband (UWB) technology commercialized in smartphones and smart keys is a high-rate pulse repetition frequency (HRP) UWB of the IEEE 802.15.4z standard, which aims to accurately determine the distance between UWB devices located within tens of meters using two-way ranging (TWR).

Drone's Angle-of-Arrival Estimation Using a Switched-Beam Antenna and Single-Channel Receiver.

In this study, we propose a method to estimate the Angle-of-Arrival (AoA) of OFDM-based drone signals with wideband and burst characteristics using only a single-channel receiver and a switched-beam antenna. First, six circularly arranged directional antennas are time-division controlled using RF switches to measure the received power of each antenna. Next, the maximum beam pattern and the measured power of each antenna are synthesized in vector form, and the direction of the synthesized vector becomes the angle of arrival of the drone signal.

Research on efficient relief logistics planning under massive natural disasters.

The frequency and intensity of massive natural disasters are increasing significantly with climate change. In response, the need and interest in disaster response systems are increasing. One of the disaster response's most important activities is delivering relief resources.

Gintonin-Enriched Extract Induces Apoptosis in Human Melanoma Cells by Causing Cell Cycle Arrest and Activating Caspases.

Gintonin, a non-saponin glycolipoprotein from , acts as a lysophosphatidic acid ligand. However, its anticancer effects, especially in melanoma, remain unclear. This study investigated the anti-proliferative effects and intracellular signaling mechanisms of a gintonin-enriched fraction (GEF) from in human melanoma cell lines.

Enhanced biocidal efficacy of alcohol based disinfectants with salt additives.

Surfaces contaminated with pathogens pose a significant risk of disease transmission and infection. Alcohol-based disinfectants are widely utilized to decontaminate high-touch areas across various settings. However, their limited antimicrobial activity and the emergence of alcohol-tolerant strains necessitate the development of highly efficient disinfectant formulations.

Gintonin-Enriched Extract Fraction Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis through DR4/5 Upregulation.

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising chemotherapeutic agent because of its selective apoptotic action on cancer cells. However, resistance to TRAIL-induced apoptosis remains a challenge in many cancers. The gintonin-enriched extract fraction (GEF) has diverse pharmacological benefits.

Evaluating layer contributions and salt coating effects on mask performance.

The impact of respiratory diseases is vast and multifaceted, affecting individuals, healthcare systems, and global economies. In response to the spread of respiratory pathogens, masks and respirators have become pivotal, demonstrating their capability to mitigate transmission. However, the limitations of conventional face masks or respirators, such as their single-use nature, environmental impact, and the risk of contact-based transmission, have accelerated the development of antimicrobial masks.

Antimicrobial Face Masks and Mask Covers with a Salt-Coated Stacked Spunbond Polypropylene Fabric: Effective Inactivation of Resilient Pathogens and Prevention of Contact Transmission.

In response to the ongoing threat posed by respiratory diseases, ensuring effective transmission protection is crucial for public health. To address the drawbacks of single-use face masks/respirators, which can be a potential source of contact-based transmission, we have designed an antimicrobial face mask and mask covering utilizing a stack of salt-coated spunbond (SB) fabric. This fabric acts as an outer layer for the face mask and as a covering over a conventional mask, respectively.

Highly Effective Salt-Activated Alcohol-Based Disinfectants with Enhanced Antimicrobial Activity.

Surfaces contaminated with pathogens raise concerns about the increased risk of disease transmission and infection. To clean biocontaminated surfaces, alcohol-based disinfectants have been predominantly used for disinfecting high-touch areas in diverse settings. However, due to its limited antimicrobial activities and concern over the emergence of alcohol-tolerant strains, much effort has been made to develop highly efficient disinfectant formulations.

Non-Invasive Vaccines: Challenges in Formulation and Vaccine Adjuvants.

Given the limitations of conventional invasive vaccines, such as the requirement for a cold chain system and trained personnel, needle-based injuries, and limited immunogenicity, non-invasive vaccines have gained significant attention. Although numerous approaches for formulating and administrating non-invasive vaccines have emerged, each of them faces its own challenges associated with vaccine bioavailability, toxicity, and other issues. To overcome such limitations, researchers have created novel supplementary materials and delivery systems.

Smartwatch gait coordination index: New measure for human gait utilizing smartwatch sensor.

Human walking reflects the state of human health. Numerous medical studies have been conducted to analyze walking patterns and to diagnose disease progression. However, this process requires expensive equipment and considerable time and manpower.

Photopolymerizable, Universal Antimicrobial Coating to Produce High-Performing, Multifunctional Face Masks.

COVID-19 poses a major threat to global health and socioeconomic structures, and the need for a highly effective, antimicrobial face mask has been considered a major challenge for protection against respiratory diseases. Here, we report the development of a universal, antiviral, and antibacterial material that can be dip-/spray-coated over conventional mask fabrics to exhibit antimicrobial activities. Our data shows that antimicrobial fabrics rapidly inactivated multiple types of viruses, i.

Development of a Whole-urine, Multiplexed, Next-generation RNA-sequencing Assay for Early Detection of Aggressive Prostate Cancer.

Background: Despite biomarker development advances, early detection of aggressive prostate cancer (PCa) remains challenging. We previously developed a clinical-grade urine test (Michigan Prostate Score [MiPS]) for individualized aggressive PCa risk prediction. MiPS combines serum prostate-specific antigen (PSA), the TMPRSS2:ERG (T2:ERG) gene fusion, and PCA3 lncRNA in whole urine after digital rectal examination (DRE).

Study of the Pathogen Inactivation Mechanism in Salt-Coated Filters.

As COVID-19 exemplifies, respiratory diseases transmitted through aerosols or droplets are global threats to public health, and respiratory protection measures are essential first lines of infection prevention and control. However, common face masks are single use and can cause cross-infection due to the accumulated infectious pathogens. We developed salt-based formulations to coat membrane fibers to fabricate antimicrobial filters.

COVID-19, flattening the curve, and Benford's law.

For many countries attempting to control the fast-rising number of coronavirus cases and deaths, the race is on to "flatten the curve," since the spread of coronavirus disease 2019 (COVID-19) has taken on pandemic proportions. In the absence of significant control interventions, the curve could be steep, with the number of COVID-19 cases growing exponentially. In fact, this level of proliferation may already be happening, since the number of patients infected in Italy closely follows an exponential trend.

Salt coatings functionalize inert membranes into high-performing filters against infectious respiratory diseases.

Respiratory protection is key in infection prevention of airborne diseases, as highlighted by the COVID-19 pandemic for instance. Conventional technologies have several drawbacks (i.e.

Neddylation inactivation represses androgen receptor transcription and inhibits growth, survival and invasion of prostate cancer cells.

Androgen receptor (AR) and its constitutively active variants (AR-Vs) have been extensively implicated in the progression and recurrence of prostate cancer, making them attractive targets in the treatment of this disease. Whether and how neddylation modification regulates AR, and the therapeutic implications of this potential regulation, are relatively unexplored areas of investigation. Here we report that neddylation inactivation by the pharmacological inhibitor MLN4924 or Lenti-shRNA-based genetic knockdown of neddylation activating enzyme (NAE) selectively suppressed growth and survival of prostate cancer cells with minor, if any, effect on normal prostate epithelial cells.

Deletions are Associated with Metastasis in African American Men with Clinically Localized, Surgically Treated Prostate Cancer.

Purpose: The potential biological determinants of aggressive prostate cancer in African American (AA) men are unknown. Here we characterize prostate cancer genomic alterations in the largest cohort to date of AA men with clinical follow-up for metastasis, with the aim to elucidate the key molecular drivers associated with poor prognosis in this population.

Experimental Design: Targeted sequencing was retrospectively performed on 205 prostate tumors from AA men treated with radical prostatectomy (RP) to examine somatic genomic alterations and percent of the genome with copy-number alterations (PGA).

Identification of TP53RK-Binding Protein (TPRKB) Dependency in -Deficient Cancers.

Tumor protein 53 (53; p53) is the most frequently altered gene in human cancer. Identification of vulnerabilities imposed by alterations may enable effective therapeutic approaches. Through analyzing short hairpin RNA (shRNA) screening data, we identified TP53RK-Binding Protein (TPRKB), a poorly characterized member of the tRNA-modifying EKC/KEOPS complex, as the most significant vulnerability in -mutated cancer cell lines.

PARP-1 regulates DNA repair factor availability.

PARP-1 holds major functions on chromatin, DNA damage repair and transcriptional regulation, both of which are relevant in the context of cancer. Here, unbiased transcriptional profiling revealed the downstream transcriptional profile of PARP-1 enzymatic activity. Further investigation of the PARP-1-regulated transcriptome and secondary strategies for assessing PARP-1 activity in patient tissues revealed that PARP-1 activity was unexpectedly enriched as a function of disease progression and was associated with poor outcome independent of DNA double-strand breaks, suggesting that enhanced PARP-1 activity may promote aggressive phenotypes.

Surface modification of paclitaxel-loaded liposomes using d-α-tocopheryl polyethylene glycol 1000 succinate: Enhanced cellular uptake and cytotoxicity in multidrug resistant breast cancer cells.

Liposomes can achieve a controlled release and an improved bioavailability of water- insoluble drug with minimized side effects. Paclitaxel is an efficient anticancer drug for the treatment of various cancers. However, paclitaxel has a solubility of 0.

The complete chloroplast genome sequence of Miq. (Aristolochiaceae), a medicinal plant in Korea.

is a medicinal plant belonging to the Aristolochiaceae family. In this study, complete chloroplast genome sequence of was characterized through assembly with next generation sequencing data. The chloroplast genome is 193,356 bp long and has the stereotypical tripartite organization consisting of large single copy region and a pair of inverted repeats.

The lncRNA landscape of breast cancer reveals a role for DSCAM-AS1 in breast cancer progression.

Molecular classification of cancers into subtypes has resulted in an advance in our understanding of tumour biology and treatment response across multiple tumour types. However, to date, cancer profiling has largely focused on protein-coding genes, which comprise <1% of the genome. Here we leverage a compendium of 58,648 long noncoding RNAs (lncRNAs) to subtype 947 breast cancer samples.

Mechanistic Support for Combined MET and AR Blockade in Castration-Resistant Prostate Cancer.

A recent phase III trial of the MET kinase inhibitor cabozantinib in men with castration-resistant prostate cancer (CRPC) failed to meet its primary survival end point; however, most men with CRPC have intact androgen receptor (AR) signaling. As previous work supports negative regulation of MET by AR signaling, we hypothesized that intact AR signaling may have limited the efficacy of cabozantinib in some of these patients. To assess the role of AR signaling on MET inhibition, we first performed an in silico analysis of human CRPC tissue samples stratified by AR signaling status ((+) or (-)), which identified MET expression as markedly increased in AR(-) samples.