Decoding information entropy of fatty acid and phospholipid vesicles ordering combinatorial output of hydrazones.

Leveraging information entropy to quantitatively measure the organizational diversity and complexity of different chemical systems is a compelling need for next-generation supramolecular and systems chemistry. It can also be a strategy for digitalizing and enabling the bottom-up development of life-like complex systems following probable origin-of-life scenarios. According to the lipid world hypothesis, lipid molecules appear first to facilitate compartmentalization, catalysis, information processing, It is envisaged that fatty acid-based vesicles are more primitive than phospholipid vesicles.

Assessing the role of PEKK implant material on cytotoxicity, inflammatory response, and molecular interactions with pro-inflammatory cytokines: An in-vitro and in-silico study.

Introduction: and aim: Due to its excellent mechanical strength and biocompatibility, Polyetherketoneketone (PEKK) is emerging as a potential substitute for titanium in dental implant applications. The aim of the study was to evaluate its cytotoxicity, pro-inflammatory responses, and molecular interactions to assess its potential in implant applications.

Methods: This study evaluated the cytotoxicity, pro-inflammatory cytokine responses, and molecular interactions of PEKK compared to titanium.

Evaluation of 3D-Printed Polylactic Acid as a Bone Substitute: An Animal Study in a Rat Model.

Objectives: Bone repair and regeneration are important processes for treating bone defects and injuries. However, traditional bone grafts like autografts and allografts have limitations, such as complications at the donor site and immune rejection. As a result, there is growing interest in using polylactic acid (PLA), a biodegradable and biocompatible material, as a synthetic bone substitute.

CAD/CAM Surgical Guide for Virtual Implant Planning and Placement: A Review.

A dental implant is a surgical fixture that is placed into the jawbone and allowed to fuse with the bone over the span of a few months. It acts as an alternative for a lacking teeth.The implant surgical guide comprises a teeth formed contour which stimulates natural teeth and prosthesis.

Comparative evaluation of the accuracy of three different three-dimensional facial scanning systems: An observational crossover study.

Aim: Facial imaging technology has become a pivotal tool in modern medical practice, particularly within fields such as maxillofacial prosthodontics, orthodontics, and smile design. The creation of digital twins, or virtual patients, enhances diagnostic accuracy, aids in treatment planning, and improves outcome prediction. The aim of the study was to assess the accuracy of various facial scanners, determine overall accuracy of each scanner, and identify which scanner demonstrates superior accuracy in specific facial regions.

Inversion of Enzymatic Enantiocatalysis Mediated by Biomolecular Condensates.

Herein, we report the enantioselective phosphodiesterase behavior of an enzyme, alkaline phosphatase (ALP) (using both R- and S-forms of the RNA-model substrate, 2-hydroxypropyl--nitrophenyl phosphate (HPNPP)), in protein (bovine serum albumin (BSA)) and DNA-based biocondensates. The native preference toward ALP enzyme is toward S-HPNPP in aqueous buffer with an enantiomeric excess ratio (E) value of around 33%. This preference for S-HPNPP remains intact when ALP is encapsulated in BSA condensate (E-value range: 10 to 34%).

Influence of L-arginine on hydroxyapatite-based ovine bone graft - An evaluation of surface characteristics and cell viability.

Background: Current challenges in bone grafting revolve around the limited availability of autografts and the complications associated with their use. Promising alternatives include osteoinductive substances stimulating stem cells to mature into bone-forming osteoblasts. However, existing products lack optimal characteristics of a bone graft.

Evaluating the Biocompatibility of Novel Green-synthesized Nano-modified Glass Ionomer Cement: A Biochemical and Histopathological Analysis Study in Wistar Albino Rats.

Aim: The study aims to evaluate the biocompatibility of novel green-synthesized nano-modified glass ionomer cement (GIC) in Wistar albino rats.

Materials And Methods: Green-synthesized chitosan, titanium, zirconia, and hydroxyapatite (HA) nanoparticles were incorporated into the GIC powder, and nine disc-shaped modified GIC specimens with a diameter of 5 mm and a thickness of 2 mm were fabricated. The specimens were then implanted subcutaneously in the dorsal region of 18 Wistar albino rats.

An Innovative Phyto-mediated Nanocomposite-modified Glass Ionomer Cement: A Detailed Study on Synthesis, Cytotoxicity, Anti-inflammatory Effects, Antimicrobial Activity, Mechanical Performance, and Solubility.

Background: Glass ionomer cement (GIC), or polyalkenoate cement, is popular for esthetic restorations due to its tooth-bonding ability but suffers from low mechanical strength, abrasion resistance, and moisture sensitivity, leading to the search for better alternatives.

Aim: To evaluate the cytotoxicity, anti-inflammatory, antimicrobial, mechanical properties, and solubility of phyto-mediated nanocomposite-modified GIC.

Materials And Methods: Chitosan-titanium-zirconia-hydroxyapatite (Ch-Ti-Zr-HA) nanocomposites were synthesized using a single-step phyto-mediated process and added to GIC at 3, 5, and 10% concentrations (groups I, II, and III), with conventional GIC as the control (group IV).

Sulfonated polyether ketone ketone (SPEKK) implant as an alternative to titanium implant-in vivo study on Wistar Albino rat mandible.

Background: Titanium is commonly used for dental implants due to its high biocompatibility and durability. However, concerns about metal ion release and aesthetic limitations in certain tissue biotypes have driven the search for alternative materials. Polyetherketoneketone (PEKK), a high-performance polymer, has emerged as a promising option due to its biomimetic properties.

An azothiazole probe as a multianalyte colorimetric chemosensor for urea and biologically significant amines.

We report an azothiazole-based probe as a chemosensor for urea with a LOD of 45 μM. The underlying sensing principle is an instantaneous color change associated with the complex forming between the probe and ammonia, a hydrolysis product of urea catalyzed by the enzyme urease. In addition, the probe has a broad scope in sensing biologically significant amines such as arginine and lysine across a wide range of pH (4 to 8).

A Critical Review of YouTube Videos on the Socket-shield Technique: A Content-quality Analysis.

Dental students often prefer social media for its accessibility and low cost but must critically evaluate the content before applying it in practice. This study analyzed YouTube content on socket shielding. A new Google account was created to search for "Socket Shield Technique" and "Partial Extraction Therapy.

Enhancing Wear Resistance in Glass Ionomer Cement through Green-mediated Chitosan-, Titanium-, Zirconium-, and Hydroxyapatite-based Nanocomposites: An Analysis before and after Chewing Simulator Endurance.

Aim And Background: Glass ionomer cement (GIC) serves as a widely used restorative dental material, known for its direct bonding to tooth structures and fluoride-releasing properties. This study aims to investigate the enhancement of GIC through the incorporation of a green-mediated nanocomposite comprising chitosan, titanium, zirconium, and hydroxyapatite, with a focus on evaluating the wear resistance of the modified GIC.

Materials And Methods: A one-pot synthesis technique was utilized to prepare a green-mediated nanocomposite incorporating chitosan, titanium, zirconium, and hydroxyapatite nanoparticles.

A double-blind randomized crossover trial comparing the esthetic outcomes of CAD-CAM provisional restorations fabricated using CBCT and IOS acquisition methods.

Objectives: This double-blind randomised crossover trial aimed to compare the aesthetic outcomes of CAD-CAM manufactured provisional restorations created using cone beam computed tomography (CBCT) and intraoral scanners (IOS) acquisition methods.

Methods: Twelve participants (mean-age: 38 ± 5 years) requiring full mouth rehabilitation were included in this crossover trial. Two sets of identical CAD-CAM provisional restorations, differing only in the method of data acquisition (A: CBCT, B: IOS), were fabricated.

Computer-aided prosthetic rehabilitation of a resected maxilla after mucormycosis using a quad zygomatic implant protocol with digital planning.

The rehabilitation of patient function and esthetics is essential from a social and psychological point of view. After a maxillary resection, the supporting structures that help provide retention or stability to a removable prosthesis are lacking. An effective, functional, straightforward, comfortable, and esthetic prosthetic rehabilitation of a patient with a resected maxilla using a digital workflow is presented.

Nucleotide-mediated modulation of chemoselective protein functionalization in a liquid-like condensed phase.

Liquid-like protein condensates are ubiquitous in cellular system and are increasingly recognized for their roles in physiological processes. Condensed phase harbors distinctive chemical microenvironment, markedly different than dilute aqueous phase. Herein, we demonstrate chemoselective modification pattern of nucleophilic canonical amino acid sidechains (namely - cysteine, tyrosine and lysine) of the protein towards 4-chloro-7-nitrobenzofurazan in the dilute and condensed phase.

Salt Gradient-Induced Phoresis of Vesicles and Enhanced Membrane Fusion in a Crowded Milieu.

Phoresis of biocolloidal objects in response to chemical gradients is a matter of interest among diverse scientific disciplines owing to their importance in the spatiotemporal orchestration of biochemical processes. Although there are reports of soft matter transport/phoresis in the gradient of ions or salts in the aqueous system, their phoretic behavior in the presence of macromolecular crowder is largely unexplored. Notably, cellular cytoplasm is illustrated as a crowded milieu and thereby understanding biomolecular phoresis in the presence of polymeric macromolecules would endorse phoretic behavior in a biomimetic environment.

Probing Phoretic Transport of Oxidative Enzyme-Bound Zn(II)-Metallomicelle in Adenosine Triphosphate Gradient via a Spatially Relocated Biocatalytic Zone.

Although cellular transport machinery is mostly ATP-driven and ATPase-dependent, there has been a recent surge in understanding colloidal transport processes relying on a nonspecific physical interaction with biologically significant small molecules. Herein, we probe the phoretic behavior of a biocolloid [composed of a Zn(II)-coordinated metallomicelle and enzymes horseradish peroxidase (HRP) and glucose oxidase (GOx)] when exposed to a concentration gradient of ATP under microfluidic conditions. Simultaneously, we demonstrate that an ATP-independent oxidative biocatalytic product formation zone can be modulated in the presence of a (glucose + ATP) gradient.

Comparative Evaluation of Microbial Adhesion on Provisional Crowns Fabricated With Milled Polymethyl Methacrylate (PMMA) and Conventional Acrylic Resin: A Prospective Clinical Trial.

Introduction Provisional prosthetic restorations play a crucial role in dentistry by protecting dentinal tubules, offering thermal insulation, and ensuring a precise fit during dental treatments. Computer-aided design and computer-aided manufacturing (CAD/CAM) have improved polymethyl methacrylate (PMMA), enhancing its mechanical properties such as hardness and resistance compared to traditional methods. However, bacterial accumulation remains a challenge due to inherent surface roughness.

Multifaceted Enhancement of L-Leucine-Enriched Ovine Bone Graft: Physicochemical Characteristics and Osteogenic Potential for Improved Guided Bone Regeneration.

Introduction Small compounds like L-leucine can boost bone regrowth by blocking certain effects, sparking cell reactions through signaling sequences. This research explored how combining L-leucine with hyaluronic acid on the developed novel graft material affects the bone's ability to conduct bone-building processes. Material and methods This study was designed as an in-vitro experiment, where a novel bone graft was formulated by integrating L-leucine with hyaluronic acid and incorporated into a hydroxyapatite-based ovine bone graft material.

Substrate-induced assembly of cascade enzymes and catalytic surfactants: nanoarchitectonics at the oil-in-water droplet interface.

The heterogeneous distribution of lipids and lipid-bound proteins in a plasma membrane has functional advantages. Herein, in a synthetic system, we demonstrate the assembly of three enzymes involved in cascade reactions, in response to the substrate of the first enzyme at the oil-water interface being stabilized by a Zn(II)-metallosurfactant. Then we show substrate-mediated catalytically-active cluster formation of the metallosurfactant in a binary mixture with another non-catalytic surfactant at the interface.

Green Synthesis and Investigation of Antimicrobial Activity and Compressive Resilience of Glass Ionomer Cement Modified With Zirconia Nanoparticles: An In Vitro Study.

Background Glass ionomer cement (GIC) serves as a crucial biomaterial in dental restoration, offering applications in filling, lining, and adhesive procedures. Nevertheless, its mechanical properties often fall short, particularly in regions subjected to considerable stress. To address this issue, zirconia nanoparticles are incorporated at specific levels.

Comparative evaluation of bond strength and color stability of polyetheretherketone and zirconia layered with indirect composite before and after thermocycling: An in vitro study.

Aim: This study investigates the interaction of zirconia and polyetheretherketone (PEEK) with indirect composite in fixed dental prostheses. This investigation aimed to assess the shear bond strength (SBS) and color stability of zirconia and PEEK before and after aging, addressing critical concerns in dental restorative applications.

Settings And Design: The current in vitro study used 96 samples, 48 of which were divided into two groups, zirconia and PEEK, before and after thermocycling.

Enzymatic Dissociation of DNA-Histone Condensates in an Electrophoretic Setting: Modulating DNA Patterning and Hydrogel Viscoelasticity.

Development of an energy-driven self-assembly process is a matter of interest for understanding and mimicking diverse ranges of biological and environmental patterns in a synthetic system. In this article, first we demonstrate transient and temporally controlled self-assembly of a DNA-histone condensate where trypsin (already present in the system) hydrolyzes histone, resulting in disassembly. Upon performing this dynamic self-assembly process in a gel matrix under an electric field, we observe diverse kinds of DNA patterning across the gel matrix depending on the amount of trypsin, incubation time of the reaction mixture, and gel porosity.

Green Synthesis, Characterization, and Evaluation of the Antimicrobial Properties and Compressive Strength of Hydroxyapatite Nanoparticle-Incorporated Glass Ionomer Cement.

Background Glass ionomer cement (GIC) plays a vital role in dental restorative procedures, serving purposes such as filling, luting, and adhesion. However, its inadequate mechanical properties pose challenges, especially in areas experiencing significant stress. To overcome this limitation, nanohydroxyapatite (nHA), known for its bioactive phosphate content, is added to the GIC at specific concentrations to improve its properties.