ALKBH5 deficiency suppresses hepatocarcinogenesis in mice via mA-dependent STAT1 restoration.

RNA mA methylation, as the most prevalent modification in mRNA, is a dynamic and reversible process primarily regulated by mA methyltransferases ("writers"), mA demethylases ("erasers"), and mA recognition proteins ("readers"). It has been shown that N6-methyladenosine (mA) plays a pivotal role in hepatocellular carcinoma (HCC). In this study we investigated the contribution of the mA eraser AlkB homolog 5 (ALKBH5) to hepatocarcinogenesis, particularly during the early stages of liver cancer development.

Single-Cell Transcriptome Analysis Uncovers Intratumoral Heterogeneity and Underlying Mechanisms for Drug Resistance in Hepatobiliary Tumor Organoids.

Molecular heterogeneity of hepatobiliary tumor including intertumoral and intratumoral disparity always leads to drug resistance. Here, seven hepatobiliary tumor organoids are generated to explore heterogeneity and evolution via single-cell RNA sequencing. HCC272 with high status of epithelia-mesenchymal transition proves broad-spectrum drug resistance.