Yolk extract-derived vitellogenin 2 ameliorates muscle atrophy in mice the PI3K/AKT/mTOR pathway.

Eggs play an important role in skeletal muscle development, but their active components are unknown. The aim of this study was to investigate the effect of yolk extract-derived vitellogenin 2 on dexamethasone (DEX)- and cancer cachexia (CC)-induced skeletal muscle atrophy. We used iTRAQ to detect the changes in protein expression between fertilized egg yolk extract (FEYE) and unfertilized egg yolk extract (UEYE).

PED-TRFIA: PEDV early on-site detection based on the time-resolved fluorescence ImmunoAssay.

Background: Porcine epidemic diarrhea virus (PEDV) infections have a significant impact on the global pig industry. To address the need for on-site detection of PEDV, the paper-based lateral flow immunoassay offers a promising approach, characterized by its simplicity of operation, rapid detection capabilities, and the elimination of expensive instrumentation. However, commonly used signal labels, such as colloidal gold nanoparticles and quantum dots have limitations in sensitivity.

An ultra-sensitive and point-of-care time-resolved fluorescence immunochromatographic platform for trace-level brucellosis antibody rapid on-site detection.

Brucellosis, a significant zoonotic disease, poses a threat to the animal husbandry industry as well as human health. To facilitate rapid and highly sensitive on-site brucellosis diagnostics, we have devised a time-resolved fluorescence immunochromatographic test strip (TRFICTS). TRFICTS employs time-resolved fluorescent microspheres (TRFMs) as both signal amplifiers and label reporters, achieving a sensitivity that is at least 12,800 times greater than the rose Bengal plate test (RBT) recommended by the World Organization for Animal Health (WOAH).

Duplex qPCR for detecting and differentiating porcine epidemic diarrhea virus GI and GII subtypes.

Introduction: Porcine epidemic diarrhea virus (PEDV) is a pathogen that causes a highly contagious intestinal disease in pigs, which causes significant economic losses to the pig industry worldwide. PCR is the most commonly used technique for PEDV diagnosis in practical clinics, however, reported works still suffer from shortcomings, for example, most of them cannot differentiate GI and GII subtypes, they suffer from low sensitivity, and some primer sequences are no longer able to match the mutant strains.

Methods: To address these issues, we conducted a comprehensive analysis by comparing the sequences of the PEDV S protein in the existing NCBI database with a recently isolated epidemic strain of PEDV, named SX0818-2022, of subtype GIIa from Shanxi, China.

Exercise-inducible circulating extracellular vesicle irisin promotes browning and the thermogenic program in white adipose tissue.

Aim: Exercise can reduce body weight and promote white fat browning, but the underlying mechanisms remain largely unknown. This study investigated the role of fibronectin type III domain-containing protein 5 (FNDC5)/Irisin, a hormone released from exercising muscle, in the browning of white fat in circulating extracellular vesicles (EVs).

Methods: Mice were subjected to a 4 weeks of running table exercise, and fat browning was analyzed via histology, protein blotting and qPCR.

Myoblast-derived exosomes promote the repair and regeneration of injured skeletal muscle in mice.

When skeletal muscle is damaged, satellite cells (SCs) are activated to proliferate rapidly and fuse with the damaged muscle fibers to form new muscle fibers, thereby promoting muscle growth and remodeling and repair of trauma. Exosomes from differentiating human skeletal muscle cells trigger myogenesis of stem cells and provide biochemical cues for skeletal muscle regeneration. Therefore, we hypothesized that, when muscles are injured, myoblast-derived exosomes may regulate muscle repair and regeneration.