Machine learning-based analysis of the impact of 5' untranslated region on protein expression.

The 5' untranslated region (5'UTR) plays a crucial regulatory role in messenger RNA (mRNA), with modified 5'UTRs extensively utilized in vaccine production, gene therapy, etc. Nevertheless, manually optimizing 5'UTRs may encounter difficulties in balancing the effects of various cis-elements. Consequently, multiple 5'UTR libraries have been created, and machine learning models have been employed to analyze and predict translation efficiency (TE) and protein expression, providing insights into critical regulatory features.

Does food availability affect nutrition-related health among rural residents in China?

Background: With the significant changes in dietary patterns, nutrition-related health issues such as overweight and obesity have become great challenges for public health in emerging economies. However, whether and how food availability could affect nutrition-related health is still unclear.

Methods: The data set comprises 14,505 observations for 8,299 individuals from the 2009-2015 China Health and Nutrition Survey (CHNS) of rural China.

The effect of food environment on nutrition-related health: evidence from rural China.

Background: Understanding the impact of the food environment on nutrition-related health is essential for addressing the rising prevalence of issues such as overweight and obesity amid rapidly changing dietary patterns in many emerging economies. This study aimed to explore the cause relationship between the food environment, including its three sub-dimensions-food availability, accessibility, and affordability, and nutrition-related health outcomes.

Methods: This study utilizes survey data collected from rural households in Shaanxi Province, China, in 2022.

Optimization of Detection in Garlic Granules Using Neutralizer-Aided Enrichment and PMAxx-qPCR.

Spices with low water activity (a) often contain antibacterial substances that complicate the detection of using traditional culture methods. These substances can drive into a viable but nonculturable (VBNC) state, rendering it undetectable with culture-based methods. This study aimed to enhance the broth for enriching in garlic granules by mitigating the inhibitory effects of antibacterial substances.

Characterization of a novel lytic phage vB_AbaM_AB4P2 encoding depolymerase and its application in eliminating biofilms formed by Acinetobacter baumannii.

Background: Acinetobacter baumannii strains are a primary cause of hospital-acquired infections. This bacterium frequently causes biofilm-related infections, notably ventilator-associated pneumonia and catheter-related infections, which exhibit remarkable resistance to antibiotic treatment, posing a severe challenge in the prevention of A. baumannii infections.

serotypes in the genomic era: simplified serotype interpretation from DNA sequence data.

In the era of genomic characterization of strains for public health microbiology, whole genome sequencing (WGS)-enabled subtyping of provides superior discrimination of strains compared to traditional methods such as serotyping. Nonetheless, serotypes are still very useful; they maintain historical continuity and facilitate clear communication. Genetic determination of serotypes from WGS data is now routine.

Isolation, evolution, and biological characterization of non-virulent and non-drug-resistant Escherichia coli from porcine intestine.

Previous studies have predominantly focused on the pathogenic mechanisms and epidemiological investigations of pathogenic Escherichia coli (E. coli), but much remains unknown about the non-virulent and non-drug-resistant E. coli (NVNR E.

Induction of Enteritidis into a Viable but Nonculturable State by Cinnamaldehyde and Its Resuscitation.

Trans-cinnamaldehyde (TC), a typical plant-derived compound, has been widely used in the control of foodborne pathogen contamination. Nevertheless, the risk associated with the occurrence of viable but nonculturable (VBNC) bacteria induced by TC remains unclear. The results of this study showed that Enteritidis ( Enteritidis) entered the VBNC state after being induced by TC at a minimum inhibitory concentration of 312.

Stress responses and Physiological Changes of Serovar Enteritidis on Short-Term and Long-Term Benzalkonium Bromide Adaptation.

is a common foodborne pathogen that poses significant safety risks across the world. And benzalkonium bromide (BK) is widely used as a disinfectant to sterilize the food processing equipment. It has been reported that sub-lethal concentration of disinfectants induced not only the homologous resistance but also cross-resistances.

Does internet use benefit the mental health of older adults? Empirical evidence from the China health and retirement longitudinal study.

The mental health (MH) of older adults is a prominent public health concern. However, research regarding the impact of emerging Internet use on MH among older adults remains limited, particularly in transitional economies experiencing a rapidly aging population such as China. Thus, to address this research gap, this study uses data from the 2013-2018 waves of the China Health and Retirement Longitudinal Study.

Heat Resistance, Virulence, and Gene Expression of Desiccation-Adapted Enteritidis During Long-Term Storage in Low-Water Activity Foods.

Desiccation stress could induce crossprotection and even affect virulence of . However, the influence of food matrices with low-water activity on desiccation adaptation of still remains unclear. This study investigated the survival and adaptation of Enteritidis in skim milk powder, ginger powder, and chocolate powder under desiccation storage conditions for a total of 12 weeks.

Is the happiness of Chinese truly the highest in the world? The impact of basic public services on happiness.

Based on the survey report by the United Nations Sustainable Development Solutions Network (SDSN) and Ipsos Group, the world ranking of Chinese people's happiness shows a significant gap. This study attempts to analyze the subjective well-being of Chinese residents through public database from the China Household Finance Survey Center in 2017. An ordered Probit model is constructed to investigate the impact of non-monetary factors, specifically basic public services, on the subjective well-being of Chinese people.

Prevalence and transmission risk of colistin and multidrug resistance in long-distance coastal aquaculture.

Due to the wide use of antibiotics, intensive aquaculture farms have been recognized as a significant reservoir of antibiotic resistomes. Although the prevalence of colistin resistance genes and multidrug-resistant bacteria (MDRB) has been documented, empirical evidence for the transmission of colistin and multidrug resistance between bacterial communities in aquaculture farms through horizontal gene transfer (HGT) is lacking. Here, we report the prevalence and transmission risk of colistin and multidrug resistance in 27 aquaculture water samples from 9 aquaculture zones from over 5000 km of subtropical coastlines in southern China.

Comparative genomics unveils extensive genomic variation between populations of Listeria species in natural and food-associated environments.

Comprehending bacterial genomic variation linked to distinct environments can yield novel insights into mechanisms underlying differential adaptation and transmission of microbes across environments. Gaining such insights is particularly crucial for pathogens as it benefits public health surveillance. However, the understanding of bacterial genomic variation is limited by a scarcity of investigations in genomic variation coupled with different ecological contexts.

Effect of honey dressing in the management of diabetic foot ulcers: A meta-analysis.

A meta-analysis study to assess the effect of honey dressing (HD) in the management of diabetic foot ulcer (DFU). A comprehensive literature examination till January 2023 was implemented and 1794 linked studies were appraised. The picked studies contained 882 subjects with DFUs were in the picked studies' baseline, 424 of them were using HD, and 458 were using a control.

Gasdermin A Is Required for Epidermal Cornification during Skin Barrier Regeneration and in an Atopic Dermatitis-Like Model.

Atopic dermatitis is featured with impaired skin barrier. The stratum corneum and the intercellular tight junctions constitute the permeability barrier, which is essential to protect water loss in the host and prevent pathogen entry. The epidermal barrier is constantly renewed by differentiating keratinocytes through cornification, during which autophagy contributes to elimination of organelles and nucleus.

Metabolomics-based response of to desiccation stress and skimmed milk powder storage.

The strong survival ability of in low-moisture foods (LMFs) has been of public concern, and is considered a threat to people's health. Recently, the development of omics technology has promoted research on the molecular mechanisms of the desiccation stress response of pathogenic bacteria. However, multiple analytical aspects related to their physiological characteristics remain unclear.

Draft Genome Sequences of Antibiotic-Resistant sp. Strains Isolated from Raw Sewage and Sediment Sludge in Georgia, USA.

Sewage has been established as a prime matrix for monitoring the emergence and dissemination of etiologic agents and antibiotic resistance determinants in a population. Here, we report the draft genomes and the acquired resistance genes of 11 antibiotic-resistant sp. isolates that were detected in samples from wastewater treatment plants.

Exploring Cantharidin and its Analogues as Anticancer Agents: A Review.

Background: Cantharidin (CTD) is a highly toxic substance which can be used to treat a variety of cancers. However, the clinical application of CTD is restricted due to the serious side effects. In recent years, screening its analogues, exploring the mechanism of action and using combinatory therapy with certain substances are considered to be feasible methods which can reduce side effects and improve the therapeutic activity of CTD.

LncRNA DUXAP8 induces breast cancer radioresistance by modulating the PI3K/AKT/mTOR pathway and the EZH2-E-cadherin/RHOB pathway.

Radiation resistance poses a major clinical challenge in breast cancer (BC) treatment, but little is known about how long noncoding RNA (lncRNA) may regulate this phenomenon. Here, we reported that DUXAP8 was highly expressed in radioresistant BC tissues, and high expression of DUXAP8 was associated with poor prognosis. We found that the overexpression of DUXAP8 promoted radioresistance, while the knockdown of DUXAP8 expression increased radiosensitivity.

Integrative Assessment of Reduced Listeria monocytogenes Susceptibility to Benzalkonium Chloride in Produce Processing Environments.

For decades, quaternary ammonium compounds (QAC)-based sanitizers have been broadly used in food processing environments to control foodborne pathogens such as Listeria monocytogenes. Still, there is a lack of consensus on the likelihood and implication of reduced susceptibility to benzalkonium chloride (BC) that may emerge due to sublethal exposure to the sanitizers in food processing environments. With a focus on fresh produce processing, we attempted to fill multiple data and evidence gaps surrounding the debate.

Draft Genome Sequences of Multidrug-Resistant Escherichia coli Isolated from River Water.

The spread of antibiotic resistance poses a critical challenge worldwide. Contaminated environments can become reservoirs, spreading antibiotic-resistant bacteria and genetic determinants of resistance to humans directly or indirectly. Here, we report the draft genome sequence, the resistome, virulence genes, and sequence types of seven multidrug-resistant Escherichia coli strains isolated from river water.