Inhibitory effects of silver and copper oxide nanoparticles, synthesized using green husk aqueous extract, on human insulin fibrillation.

Recent research indicates that nanoparticles can serve as tools for the diagnosis and treatment of diseases. This study investigates the inhibitory effects of silver and copper oxide nanoparticles, synthesized using green husk aqueous extract, on human insulin fibrillation. Initially, the formation of amyloid fibrils in recombinant human insulin protein was examined under various buffers and by altering physicochemical conditions, such as pH and temperature, identifying optimal conditions for fibril formation.

Synthesis and in vitro quantification of amyloid fibrils by barbituric and thiobarbituric acid-based chromene derivatives.

Neurodegenerative disease is caused by the abnormal build-up of proteins in and around cells called amyloid. The amyloid fibril formation and its mechanism have been investigated with various techniques, including dye-binding assay. Thioflavin T (ThT) has been one of the most widely used dyes for quantifying amyloid deposits, but ThT has a weak fluorescence signal especially at low concentration of amyloid fibrils, low lipophilicity and positive charge that makes it unable to cross the blood-brain barrier (BBB) to detect amyloid fibrils in vivo.