C/H/O stable isotope of rape honey and its components combined with machine learning algorithms to identify geographical origin.

Rationale: The distribution of rape honey is among the largest and most diverse of all honeys available to humankind with respect to the geographical origin. Accurate isotopic reference values for rape honey are therefore important for precise verification of honey origin and its traceability. New combined rape honey δ C, δ H, and δ O values in combination with values on its compounds (protein and saccharides) were used to complement existing databases to better identify the geographical origin of Chinese rape honey.

Study on stable carbon isotope fractionation of rape honey from rape flowers (Brassica napus L.) to its unifloral ripe honey.

A new idea and strategy for honey traceability and identification was provided by studying the carbon isotope fractionation of rape honey and its components in the different ripening process, as well as the fractionation from rape flowers, stamens, nectar to rape honey. The results showed the moisture content of rape honey continued to decrease, and the glucose and fructose content continued to increase during the ripening process. The δC of rape honey and its protein were less affected by honey ripeness, while the δC of sugars in rape honey were greatly affected by this.

Discrimination of geographical origins of Chinese acacia honey using complex C/C, oligosaccharides and polyphenols.

The aim of this study was to predict the geographical origin of acacia honey of China through analysis of physicochemical parameters combination with chemometrics. Samples from six different origins were investigated on parameters of δC value, oligosaccharides and polyphenols, using EA-IRMS/LC-IRMS, GC-MS and HPLC-MS, respectively. The results indicated that the δC value of honey from Gansu region were lower than those of other regions.