Anti-PD-1 treatment response is associated with the influx of circulating myeloid and T-cell subsets into the metastatic melanoma tumor microenvironment.

Background: Immune checkpoint inhibition (ICI) significantly improves the survival of metastatic melanoma patients; however, a substantial proportion of patients does not respond to these breakthrough therapies.

Methods: To improve our understanding of this response variability, we developed high-plex panels for protein imaging of a discovery cohort and validation with RNAseq analyses to examine myeloid and T-cell subsets in pre-anti-PD-1-treatment samples of 14 metastatic melanoma patients (7 responders and 7 non-responders).

Results: We demonstrate that a higher abundance of circulating monocyte-derived macrophages (MDMs) and cytotoxic T-cell subsets in the tumor microenvironment (TME) at baseline distinguishes metastatic melanoma patients with a favorable response to anti-PD1 treatment from non-responders, who featured co-localization of suppressive macrophages (M2) and T-cells.

Capturing the three-dimensional, nanoscale, picosecond dynamics of plasma mirrors with intense ultrashort laser wavefront measurement.

We present a direct measurement of the nanoscale dynamics of plasma mirrors using wavefront measurement techniques. This two-dimensional measurement, performed via pump-probe diagnostics, enables the reconstruction of the three-dimensional plasma mirror surface with nanometer axial, micrometer transverse, and femtosecond temporal resolution.

Investigating the potent antimicrobial properties of a supramolecular Zn(ii)-metallogel formed from an isophthalic acid-based low molecular weight gelator.

A zinc(ii)-based metallogel was synthesized using isophthalic acid as a gelator in ,-dimethylformamide at room temperature. The gel formation was confirmed by FT-IR spectroscopy, revealing coordination interactions responsible for network assembly. Rheological studies demonstrated the mechanical robustness and stability of the Zn(ii)-metallogel under various conditions.

Beyond benchmarking: an expert-guided consensus approach to spatially aware clustering.

Spatial omics technologies have revolutionized the study of tissue architecture and cellular heterogeneity by integrating molecular profiles with spatial localization. In spatially resolved transcriptomics, delineating higher-order anatomical structures is critical for understanding how cellular organization affects tissue and organ function. Since 2020, more than 50 spatially aware clustering (SAC) methods have been developed for this purpose.

A Robust Nickel-Interlocked π-Conjugated Covalent Organic Framework Catalyst for Photocatalytic Aromatic Finkelstein and Retro-Finkelstein Reactions.

Covalent organic frameworks (COFs) are emerging as a versatile class of hosts for heterogeneous photocatalysis. Herein, we present a nickel-decorated pyrene- and bipyridine-based olefin-linked COF (Ni@COF1) as a robust and recyclable catalyst for visible-light-driven aromatic Finkelstein and retro-Finkelstein reactions. The extended π-conjugation within the COF framework enhances light absorption, promotes charge transport, and facilitates in situ Ni(0) generation from the pre-installed Ni(II) centers.