AcrAB Efflux Pump Plays a Crucial Role in Bile Salts Resistance and Pathogenesis of .

Bile salts possess innate antibacterial properties and can cause significant damage to bacteria. To survive in the mammalian gut, has developed mechanisms to tolerate bile salts; however, the specific mechanisms remain unclear. Transposon library screening revealed that the efflux pump AcrAB is involved in bile salt resistance.

Legionella effectors SidC/SdcA ubiquitinate multiple small GTPases and SNARE proteins to promote phagosomal maturation.

Protein ubiquitination is one of the most important posttranslational modifications (PTMs) in eukaryotes and is involved in the regulation of almost all cellular signaling pathways. The intracellular bacterial pathogen Legionella pneumophila translocates at least 26 effectors to hijack host ubiquitination signaling via distinct mechanisms. Among these effectors, SidC/SdcA are novel E3 ubiquitin ligases with the adoption of a Cys-His-Asp catalytic triad.

Bacterial ubiquitin ligases hijack the host deubiquitinase OTUB1 to inhibit MTORC1 signaling and promote autophagy.

Many bacterial pathogens have evolved effective strategies to interfere with the ubiquitination network to evade clearance by the innate immune system. Here, we report that OTUB1, one of the most abundant deubiquitinases (DUBs) in mammalian cells, is subjected to both canonical and noncanonical ubiquitination during infection. The effectors SidC and SdcA catalyze OTUB1 ubiquitination at multiple lysine residues, resulting in its association with a -containing vacuole.

Ubiquitination of Sec22b by a novel ubiquitin E3 ligase.

Protein ubiquitination is one of the most important post-translational modifications that plays critical roles in the regulation of a wide range of eukaryotic signaling pathways. Many successful intracellular bacterial pathogens can hijack host ubiquitination machinery through the action of effector proteins that are injected into host cells by secretion systems. is the etiological agent of legionellosis that is able to survive and replicate in various host cells.

The phospholipase effector Tle1 promotes virulence by killing competitors and impacting gene expression.

utilizes the Type VI secretion system (T6SS) to gain an advantage in interbacterial competition by delivering anti-prokaryotic effectors in a contact-dependent manner. However, the impact of T6SS and its secreted effectors on physiological behavior remains poorly understood. In this study, we present Tle1, a phospholipase effector in atypical pathogenic E1 that is secreted by T6SS via its interaction with VgrG1.

PAS Domain-Containing Chemoreceptors Influence the Signal Sensing and Intestinal Colonization of .

Bacterial chemotaxis is the phenomenon in which bacteria migrate toward a more favorable niche in response to chemical cues in the environment. The methyl-accepting chemotaxis proteins (MCPs) are the principal sensory receptors of the bacterial chemotaxis system. Aerotaxis is a special form of chemotaxis in which oxygen serves as the signaling molecule; the process is dependent on the aerotaxis receptors (Aer) containing the Per-Arnt-Sim (PAS) domain.

-Mediated In Vitro Inhibition of Plasmid Transfer Is Reversed by the Intestinal Environment.

Colistin is regarded as an antibiotic of last resort against multidrug-resistant Gram-negative bacteria, including and . Colistin resistance is acquired by microorganisms via chromosome-mediated mutations or plasmid-mediated mobile colistin resistance () gene, in which the transfer of is the predominant factor underlying the spread of colistin resistance. However, the factors that are responsible for the spread of the gene are still unclear.

Bacteriophage SRD2021 Recognizing Capsular Polysaccharide Shows Therapeutic Potential in Serotype K47 Infections.

is an opportunistic pathogen posing an urgent threat to global public health, and the capsule is necessary for infection and virulence. Phage-derived capsule depolymerases have shown great potential as antivirulence agents in treating carbapenem-resistant (CRKP) infections. However, the therapeutic potential of phages encoding depolymerases against CRKP remains poorly understood.

O-antigen serves as a two-faced host factor for bacteriophage NJS1 infecting nonmucoid Klebsiella pneumoniae.

Klebsiella pneumoniae is an opportunistic pathogen commonly associated with nosocomial infections. In our previous study, we have demonstrated that colistin-resistant K. pneumoniae is more susceptible to killing by lytic tailed phages than the colistin-sensitive parent strain, including T1-like ФNJS1.

Isolation and characterization of a bacterium able to degrade high concentrations of iprodione.

A bacterial strain CQH-1 capable of mineralizing iprodione was isolated and characterized. On the basis of its morphological, physiological, and biochemical characteristics combined with phylogenetic analysis of its 16S rRNA gene sequence, strain CQH-1 was identified as a Microbacterium sp. CQH-1.