CD44 and Snail1 Expression Predicts Poor Prognosis of Oral Squamous Cell Carcinoma.

Background: Oral squamous cell carcinoma (OSCC) remains a challenging malignancy with poor 5-year survival rates due to diagnosis at an advanced stage and a high likelihood of recurrence and metastasis. These aggressive traits may be influenced by cancer stem cells (CSC) and epithelial-mesenchymal transition (EMT).

Methods: This study investigated the prognostic significance of the CSC marker CD44 and EMT-related proteins (Snail1, Snail2, E-cadherin, N-cadherin) in 132 OSCCs using immunohistochemistry.

Prognostic Value of the Immunohistochemical Detection of Cellular Components of the Tumor Microenvironment in Oral Squamous Cell Carcinoma: A Systematic Review.

This study aims to investigate the prognostic impact of cellular components of the tumor microenvironment (TME), analyzed through immunohistochemistry, in oral squamous cell carcinoma (OSCC). This review was conducted following the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Searches were performed in EMBASE, Medline/PubMed, Cochrane Collaboration Library, Web of Science, ScienceDirect, Scopus, and Google Scholar.

Mortality Associated with Orofacial Clefts in Brazil.

Orofacial clefts are congenital anomalies that cause substantial morbidity and mortality. This study aimed to investigate temporal and geographic trends in mortality among Brazilian individuals with orofacial clefts listed as the underlying cause of death on death certificates. A retrospective cross-sectional study was conducted using data from the Department of Informatics of the Brazilian Unified Health System (DATASUS) from 1996 to 2023.

Knowledge and attitudes about rare genetic diseases among practitioners of oral medicine/pathology in Brazil: a cross-sectional study.

Introduction: This study aimed to analyze the knowledge and attitudes of Brazilian Oral Medicine and Pathology (OM/OP) specialists about genetic diseases.

Methods: A cross-sectional and descriptive study was conducted with Brazilian OM/OP specialists, using a pre-structured online formulary. Statistical analyses were performed using Statistical Package for the Social Sciences (SPSS®).

3D Bioprinting Skin Equivalents: A Methodological Perspective on Human Keratinocyte and Fibroblast Models for Wound Repair and Regeneration.

Three-dimensional (3D) bioprinting is a promising approach to developing reliable tissue substitutes for translational research. The great interest in creating skin substitutes still faces challenges considering its structural and cellular complexity. Despite significant advancements, the lack of reproducible protocols and different translational barriers limit the clinical applicability of current methods.

Validation of a New Histopathologic Risk Model in Early Oral Tongue Cancer: A Combination of a Modified Worst Pattern of Invasion and a New Tumor Budding Score.

Oral tongue squamous cell carcinoma (OTSCC) is the most common cancer of the oral cavity. A new histopathologic risk assessment has been recently introduced and we sought to validate its prognostic value in a large multicenter cohort of early-stage OTSCC. A total of 310 cases treated for early-stage OTSCC were included in this study.

A method for dissociation of oral cancer cell spheroids generated by magnetic 3D bioprinting.

Unlabelled: Spheroid 3D cultures yield a better representation of diverse cellular events in comparison with 2D cultures. However, the ability to investigate some specific phenotype/cell biology-related outcomes of experimental interventions by flow cytometry is somewhat limited in spheroid cultures, as it requires a single cell suspension with good preservation of membrane-bound proteins. In this study, we describe a method combining enzymatic and mechanical dissociation of large spheroids of five different oral squamous cell carcinoma (OSCC) cell lines (H314, SCC9, SCC25, UM-SCC1 and HSC3).

Temporomandibular joint disorders, happiness, and COMT, HTR2A and FKBP5 polymorphisms in adolescents: a cross-sectional study.

Temporomandibular joint disorder (TMD) has a multifactorial etiology involving psychological and genetic aspects. This condition commonly begins in adolescence, which is a period of emotional, physical, and psychological maturation. The aim of the present study was to investigate associations between temporomandibular joint (TMJ) disorder and happiness as well as polymorphisms in the COMT, HTR2A, and FKBP5 genes in Brazilian adolescents.

Investigating possible shared single nucleotide polymorphisms in isolated oral cleft and non-cleft facial morphology.

Introduction: Facial morphogenesis is regulated by several cellular interactions that are mediated by numerous morphogenetic signals. Based on the existing evidence, we hypothesize that oral cleft-associated single nucleotide polymorphisms (SNPs) are involved in the normal range of human face development. Therefore, this study aimed to investigate the association between SNPs in oral cleft-related genes and variations in the normal range of facial morphology.

A Large Multicenter Brazilian Case-Control Study Exploring Genetic Variations in Interferon Regulatory Factor 6 and the Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate.

Nonsyndromic cleft lip with or without cleft palate (NSCL ± P) is strongly associated with both environmental and genetic risk factors, but its genetic underpinnings remain partially known. While variants in interferon regulatory factor 6 (IRF6) are linked to NSCL ± P risk in populations from Asia and Europe, studies on the highly admixed Brazilian population are scarce and have produced ambiguous results. This study aimed to investigate the contribution of variants to the risk of NSCL ± P.

Exploring polymorphisms in genes encoding growth factors associated with non-syndromic cleft lip with or without cleft palate and tooth agenesis.

Objective: To evaluate the association between non-syndromic cleft lip with or without cleft palate (NSCL±P) and tooth agenesis (TA), as well as the association of both conditions with polymorphisms in genes encoding growth factors.

Methodology: This cross-sectional study included children with NSCL±P and a control group of children without NSCL±P. Permanent teeth TA (excluding third molars) was evaluated using panoramic radiographs by a trained examiner.

Stromal Prognostic Markers for Oral Cancer: An Update.

Background: This article aims to provide a broad overview of the oral squamous cell carcinoma (OSCC) tumor stroma, describing and discussing recent advances in the understanding of different stromal elements and their roles in tumor progression. We also describe potential new therapeutic approaches targeting the stroma.

Methods: A literature review on the role of stromal biomarkers in OSCC was conducted.

Family History of Nonsyndromic Orofacial Clefts: A Brazilian Multicenter Study.

Objective: To summarize the evidence on the relationship between hereditary family history and nonsyndromic orofacial clefts (NSOC) in patients from various Brazilian states.

Methods: This cross-sectional multicenter study was conducted at six specialized orofacial cleft services across different regions of Brazil. The sample consisted of 1899 patients with NSOC, including cleft lip only (NSCLO), cleft palate only (NSCPO), and cleft lip and palate (NSCLP).

PETRICCS Guideline Protocol: A Call to Improve Reporting Standards in Cell Culture Research.

Background: Cell culture studies play an important role in addressing fundamental scientific questions. However, inadequate reporting of these studies results in a lack of transparency and reproducibility. Recognizing the need for improvement, several ongoing efforts, such as CRIS guidelines and the ICLAC checklist, are focused on enhancing best practices for in vitro studies.

Tooth Anomalies in Patients With Nonsyndromic Orofacial Cleft: A Systematic Review and Meta-Analysis.

Objective: To evaluate the frequency of tooth anomalies (TA) in the deciduous and permanent dentition of patients with nonsyndromic orofacial clefts (NSOC), both inside and outside the cleft area.

Methods: The following databases were searched for the relevant literature: Cochrane, OVID, SciELO, Embase, Livivo, PubMed, Scopus, and Web of Science. The risk of bias was analyzed using the Joanna Briggs Institute.

Scientific contribution of the Brazilian CNPq Research Productivity fellows in dentistry.

This study evaluated the scientific contribution of Brazilian CNPq Research Productivity fellows (PQ) in Dentistry by areas of activity. This cross-sectional study included 217 active PQ who were grouped into six groups: (1) Biomaterials, Prosthodontics, and Restorative Dentistry; (2) Public Health and Epidemiology; (3) Oral Pathology, Stomatology, and Dental Radiology; (4) Pediatric Dentistry and Child Health; (5) Dental Clinic (Periodontics, Endodontics, Orthodontics, Oral Surgery, and Implantology), and (6) Basic Areas (Histology, Biochemistry, Molecular Biology, Physiology, Microbiology, Immunology, and Pharmacology). The PQ were predominantly male (n = 122; 56.

Methylome-wide analysis in systemic microbial-induced experimental periodontal disease in mice with different susceptibility.

Objective: The study delved into the epigenetic factors associated with periodontal disease in two lineages of mice, namely C57bl/6 and Balb/c. Its primary objective was to elucidate alterations in the methylome of mice with distinct genetic backgrounds following systemic microbial challenge, employing high-throughput DNA methylation analysis as the investigative tool.

Methods: ()was orally administered to induce periodontitis in both Balb/c and C57bl/6 lineage.

Web-based prognostic tools for oral tongue cancer: An analysis of online predictors.

Background: Oral tongue squamous cell carcinoma (OTSCC) often presents with aggressive clinical behaviour that may require multimodality treatment based on reliable prognostication. We aimed to evaluate the prognostic ability of five online web-based tools to predict the clinical behaviour of OTSCC resection and biopsy samples.

Methods: A total of 135 OTSCC resection cases and 33 OTSCC biopsies were included to predict recurrence and survival.