Alterations in 3D Chromatin Spatial Organisation in Tumourigenesis and Therapy Resistance of Glioblastoma: The Recent Advances in Understanding Molecular Mechanisms, Clinical Implications, and Therapeutic Perspectives.

Glioblastoma (GBM) remains one of the most aggressive and lethal forms of brain cancer, characterised by profound genetic, epigenetic, and phenotypic heterogeneity. Recent advancements in high-resolution genome mapping have unveiled the critical role of three-dimensional (3D) chromatin architecture-encompassing chromatin loops, topologically associating domains, and enhancer-promoter interactions-in driving GBM tumourigenesis and therapy resistance. This review summarises recent insights into the mechanistic contribution of 3D genome reorganisation in sustaining oncogenic transcriptional programs, promoting intratumoural heterogeneity, and facilitating adaptive resistance.

CRISPR/Cas9 in colorectal cancer: Revolutionizing precision oncology through genome editing and targeted therapeutics.

Colorectal cancer (CRC) remains a significant global health challenge, necessitating advanced molecular therapies to improve outcomes. The CRISPR/Cas9 genome-editing platform has emerged as a transformative tool in CRC research, enabling precise genomic modifications to suppress tumor progression, enhance chemosensitivity, and modulate oncogenic pathways. This review highlights CRISPR/Cas9 applications in CRC models, including MC38 murine and CaCO-2 cell lines, where targeted gene edits demonstrate tumor-suppressive effects.

Nanotechnology-Enabled Antibiotic Therapy: The Promise of Transfersomal Tigecycline in Combating Methicillin-Resistant Staphylococcus aureus.

Introduction/objective: Methicillin-Resistant Staphylococcus Aureus (MRSA) is a major cause of purulent Skin and Soft-Tissue Infections (SSTIs), posing significant global health and economic challenges. This study aims to optimize a drug delivery system, specifically Tigecyclineloaded transfersomes, to address the limitations of current treatments, including bacterial resistance, systemic side effects, and poor drug penetration, thereby offering a safer and more effective alternative for MRSA-related SSTIs.

Methods: A novel Tigecycline transfersomal formulation was developed using the thin film hydration method.

TIGIT, as a potential immune checkpoint target for immunotherapy of breast cancer.

Breast cancer ranks among the most critical cancers globally due to its elevated prevalence, with an excess of 2.3 million newly reported cases, and it's devastating toll, resulting in a substantial number of fatalities annually. Its significance stems from its complex origins, which include intersecting genetic mutations like breast cancer type 1 susceptibility protein 1/2 (BRCA1/2) that impair DNA repair and heighten hereditary risk, alongside hormonal factors and lifestyle influences.

EXPRESS: Liquid Biopsy in Breast Cancer Management: From Circulating Biomarkers to Clinical Breakthroughs.

Breast cancer continues to be a major global health concern, particularly for women, despite improvements in early detection and treatment strategies. Traditional tissue biopsies have long been the foundation of diagnosis and treatment planning; however, they come with limitations-such as being invasive, providing only a single snapshot of a dynamic disease, and often missing the evolving molecular landscape, especially in metastatic cases. In recent years, liquid biopsy has emerged as a powerful, non-invasive tool offering real-time insights into tumor biology.