Publications by authors named "Oleksandr Dovzhenko"
Article Synopsis
- This study explores the use of droplet-based microfluidics to cultivate microspores via doubled haploid technology, which is crucial for plant breeding as it helps embed desired traits genetically.
- The researchers investigated various factors like cell density and heat shock conditions to enhance microspore viability and reprogramming rates, finding improved results at specific temperatures over a few days.
- Ultimately, the method allows for efficient screening of optimal growth conditions and enables the generation of mature plants from developed embryos, indicating its potential for automation in plant research.
Article Synopsis
- High-throughput microscopy was used to analyze isolated tobacco leaf protoplasts, offering insights into the cell growth and development of single plant cells, which has not been extensively studied before.
- * The study focused on measuring early developmental responses, such as cell expansion and growth rates, of protoplasts with and without the expression of an Arabidopsis protein called AtBAG4.
- * Protoplasts expressing AtBAG4 exhibited enhanced growth and proliferation rates, suggesting that this protein may increase cell resilience and survival, potentially through mechanisms related to potassium channel abundance.
Molecular Control of Sporophyte-Gametophyte Ontogeny and Transition in Plants.
Front Plant Sci
January 2022
Alternation of generations between a sporophytic and gametophytic developmental stage is a feature common to all land plants. This review will discuss the evolutionary origins of these two developmental programs from unicellular eukaryotic progenitors establishing the ability to switch between haploid and diploid states. We will compare the various genetic factors that regulate this switch and highlight the mechanisms which are involved in maintaining the separation of sporophytic and gametophytic developmental programs.
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